Restriction map for
pF1KE0138
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 162 GGCGCC G[GA]^CG[CT]C
AocI 369 CCTCAGG CC^T[ACGT]AGG
ApaI 781 GGGCCC GGGCC^C
AspHI 555 GTGCTC G[AT]GC[AT]^C
AspI 592 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 620 TGGCCA TGG^CCA
BanII 781 GGGCCC G[GA]GC[CT]^C
BbeI 162 GGCGCC GGCGC^C
BbsI 538 GAAGAC GAAGAC(2/6)
BfaI 464 CTAG C^TAG
BpuAI 538 GAAGAC GAAGAC(2/6)
BsaHI 162 GGCGCC G[GA]^CG[CT]C
BsaI 121 GAGACC GGTCTC(1/5)
BsiEI 227 CGGCCG CG[GA][CT]^CG
BsiHKAI 555 GTGCTC G[AT]GC[AT]^C
BsmAI 121 GAGAC GTCTC(1/5)
BsmFI 41 GTCCC GGGAC(10/14)
BssSI 321 CTCGTG CACGAG(-5/-1)
BstXI 137 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 369 CCTCAGG CC^T[ACGT]AGG
DraIII 334 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 338 CCGTGG C^C[GA][CT]GG
EagI 227 CGGCCG C^GGCCG
EarI 217 CTCTTC CTCTTC(1/4)
EclXI 227 CGGCCG C^GGCCG
Eco52I 227 CGGCCG C^GGCCG
Eco81I 369 CCTCAGG CC^T[ACGT]AGG
EcoNI 90 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 162 GGCGCC G[GA]^CG[CT]C
HinfI 407 GAGTC G^A[ACGT]TC
HphI 302 TCACC GGTGA(8/7)
KasI 162 GGCGCC G^GCGCC
Ksp632I 217 CTCTTC CTCTTC(1/4)
MaeI 464 CTAG C^TAG
MaeII 596 ACGT A^CGT
MaeIII 521 GTGAC ^GT[ACGT]AC
MluNI 620 TGGCCA TGG^CCA
MscI 620 TGGCCA TGG^CCA
NarI 162 GGCGCC GG^CGCC
NspI 266 GCATGC [GA]CATG^[CT]
PflFI 592 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 407 GAGTC GAGTC(4/5)
PstI 607 CTGCAG CTGCA^G
SfcI 607 CTGCAG C^T[GA][CT]AG
SfiI 205 GGCCAGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 162 GGCGCC GGC^GCC
SmaI 776 CCCGGG CCC^GGG
SmlI 440 CTCAAG C^T[CT][GA]AG
SphI 266 GCATGC GCATG^C
StuI 572 AGGCCT AGG^CCT
TaqI 236 TCGA T^CGA
Tsp45I 521 GTGAC ^GT[GC]AC
Tth111I 592 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 236 TCGA T^CGA
XmaCI 776 CCCGGG C^CCGGG
XmaI 776 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 132 GTAC GT^AC
195 GTAC
AvaI 157 CCCGAG C^[CT]CG[GA]G
776 CCCGGG
BanI 162 GGCGCC G^G[CT][GA]CC
276 GGTGCC
BglI 109 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
206 GCCAGGGGGGC
BsgI 151 CTGCAC GTGCAG(16/14)
565 GTGCAG
BsoBI 157 CCCGAG C^[CT]CG[GA]G
776 CCCGGG
BspMI 182 ACCTGC ACCTGC(4/8)
800 ACCTGC
NaeI 202 GCCGGC GCC^GGC
283 GCCGGC
NgoMIV 202 GCCGGC G^CCGGC
283 GCCGGC
NlaIII 267 CATG CATG^
552 CATG
PflMI 333 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCAGTGACTGG
PpuMI 39 GGGTCCC [GA]G^G[AT]CC[CT]
676 AGGTCCT
RsaI 132 GTAC GT^AC
195 GTAC
SfaNI 240 GATGC GCATC(5/9)
421 GATGC
Tsp509I 385 AATT ^AATT
412 AATT
TspRI 519 CAGTG CA[GC]TG[ACGT][ACGT]^
735 CAGTG
Van91I 333 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCAGTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PvuII
Sau96I ScrFI TseI