Restriction map for
pF1KB5886
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BanI BbeI BbvCI BciVI BclI
BfrI BglII BsaBI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 373 GTCTAC GT^[AC][GT]AC
AccII 838 CGCG CG^CG
AccIII 413 TCCGGA T^CCGGA
AfaI 633 GTAC GT^AC
AvrII 774 CCTAGG C^CTAGG
BamHI 615 GGATCC G^GATCC
BbrPI 943 CACGTG CAC^GTG
BcgI 1080 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 774 CCTAGG C^CTAGG
BsaAI 943 CACGTG [CT]AC^GT[GA]
BsaI 676 GGTCTC GGTCTC(1/5)
BseAI 413 TCCGGA T^CCGGA
BsmBI 4 GAGACG CGTCTC(1/5)
BspEI 413 TCCGGA T^CCGGA
BssSI 220 CACGAG CACGAG(-5/-1)
BstUI 838 CGCG CG^CG
CfoI 799 GCGC GCG^C
DraIII 1183 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 551 CCGTGG C^C[GA][CT]GG
EcoRI 733 GAATTC G^AATTC
HhaI 799 GCGC GCG^C
HinP1I 799 GCGC G^CGC
MaeII 944 ACGT A^CGT
MroI 413 TCCGGA T^CCGGA
MseI 1076 TTAA T^TAA
MvnI 838 CGCG CG^CG
PleI 417 GACTC GAGTC(4/5)
PmaCI 943 CACGTG CAC^GTG
PmlI 943 CACGTG CAC^GTG
PstI 493 CTGCAG CTGCA^G
RsaI 633 GTAC GT^AC
SfiI 1431 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 1023 GATTC G^A[AT]TC
Tru9I 1076 TTAA T^TAA
XcmI 557 CCAAGAGATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 442 GAATTT [GA]^AATT[CT]
733 GAATTC
AlwNI 826 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
910 CAGTTCCTG
ApoI 442 GAATTT [GA]^AATT[CT]
733 GAATTC
Asp700I 405 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAGAGATTC
AvaII 1208 GGACC G^G[AT]CC
1421 GGACC
BbsI 810 GAAGAC GAAGAC(2/6)
877 GTCTTC
BfaI 35 CTAG C^TAG
775 CTAG
BlpI 228 GCTGAGC GC^T[ACGT]AGC
757 GCTGAGC
Bpu1102I 228 GCTGAGC GC^T[ACGT]AGC
757 GCTGAGC
BpuAI 810 GAAGAC GAAGAC(2/6)
877 GTCTTC
BsaWI 413 TCCGGA [AT]^CCGG[AT]
719 TCCGGT
BsmFI 1207 GGGAC GGGAC(10/14)
1420 GGGAC
BsrBI 126 CCGCTC CCGCTC(-3/-3)
1123 GAGCGG
BsrFI 204 GCCGGC [GA]^CCGG[CT]
1409 GCCGGC
BstAPI 219 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
695 GCAAACAGTGC
CelII 228 GCTGAGC GC^T[ACGT]AGC
757 GCTGAGC
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
1409 GCCGGC
EarI 454 GAAGAG CTCTTC(1/4)
1018 GAAGAG
EcoNI 915 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1164 CCTCATTGAGG
HgaI 839 GCGTC GACGC(5/10)
1368 GCGTC
HinfI 417 GACTC G^A[ACGT]TC
1023 GATTC
Ksp632I 454 GAAGAG CTCTTC(1/4)
1018 GAAGAG
MaeI 35 CTAG C^TAG
775 CTAG
NaeI 204 GCCGGC GCC^GGC
1409 GCCGGC
NgoMIV 204 GCCGGC G^CCGGC
1409 GCCGGC
PpuMI 1207 GGGACCC [GA]G^G[AT]CC[CT]
1420 GGGACCC
PvuII 496 CAGCTG CAG^CTG
889 CAGCTG
SacI 172 GAGCTC GAGCT^C
1312 GAGCTC
SfcI 493 CTGCAG C^T[GA][CT]AG
1414 CTACAG
Tsp509I 443 AATT ^AATT
734 AATT
XmnI 405 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
BalI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
StyI TaqI TseI TspRI TthHB8I
XhoII XmaCI XmaI