Restriction map for
pF1KA0533
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# NO CUT ENZYMES ( 38 enzymes) #
##################################
AclI AflII AseI AsnI Asp700I
AvrII BfrI BlnI BsiWI Bst1107I
BstBI BstZ17I CpoI DraI EcoRI
EcoT22I HindIII HpaI MluI MseI
NdeI NruI NsiI NspV PacI
PmeI PshBI Psp1406I PvuI RsrII
SfuI SnaBI SpeI SspI SwaI
Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AcsI 726 GAATTT [GA]^AATT[CT]
AhdI 5107 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 726 GAATTT [GA]^AATT[CT]
AscI 9365 GGCGCGCC GG^CGCGCC
AspEI 5107 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 7012 GCTGAGG CCTCAGC(-5/-2)
BclI 3260 TGATCA T^GATCA
BsaBI 5223 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 6682 GAGACG CGTCTC(1/5)
BsmI 1987 GAATGC GAATGC(1/-1)
Eam1105I 5107 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 877 GATATC GAT^ATC
FbaI 3260 TGATCA T^GATCA
KspI 402 CCGCGG CCGC^GG
MamI 5223 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6787 CAATTG C^AATTG
MunI 6787 CAATTG C^AATTG
NheI 10806 GCTAGC G^CTAGC
NotI 5795 GCGGCCGC GC^GGCCGC
PaeR7I 2686 CTCGAG C^TCGAG
PshAI 2532 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 402 CCGCGG CCGC^GG
SalI 8799 GTCGAC G^TCGAC
SexAI 5820 ACCTGGT A^CC[AT]GGT
SgrAI 1200 CACCGGCG C[GA]^CCGG[CT]G
TfiI 5861 GATTC G^A[AT]TC
XhoI 2686 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 8326 GGTACC G^GTACC
8639 GGTACC
AccIII 6656 TCCGGA T^CCGGA
6938 TCCGGA
AgeI 1850 ACCGGT A^CCGGT
10997 ACCGGT
Asp718I 8326 GGTACC G^GTACC
8639 GGTACC
AspI 1538 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
8617 GACTTCGTC
BamHI 2199 GGATCC G^GATCC
10307 GGATCC
BciVI 3468 GGATAC GTATCC(6/5)
4420 GGATAC
BglII 5402 AGATCT A^GATCT
6011 AGATCT
BseAI 6656 TCCGGA T^CCGGA
6938 TCCGGA
BspDI 361 ATCGAT AT^CGAT
8458 ATCGAT
BspEI 6656 TCCGGA T^CCGGA
6938 TCCGGA
BspHI 5348 TCATGA T^CATGA
5696 TCATGA
ClaI 361 ATCGAT AT^CGAT
8458 ATCGAT
FseI 1803 GGCCGGCC GGCCGG^CC
7134 GGCCGGCC
KpnI 8326 GGTACC GGTAC^C
8639 GGTACC
MroI 6656 TCCGGA T^CCGGA
6938 TCCGGA
PflFI 1538 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
8617 GACTTCGTC
PinAI 1850 ACCGGT A^CCGGT
10997 ACCGGT
RcaI 5348 TCATGA T^CATGA
5696 TCATGA
ScaI 302 AGTACT AGT^ACT
653 AGTACT
Tth111I 1538 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
8617 GACTTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 172 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AspHI AvaI AvaII AviII BalI
BanI BanII BbeI BbrPI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI SphI Sse8387I SspBI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI