Restriction map for
pF1KB5816
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
AspI BalI BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BsaAI
BsaBI BsgI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EcoRI
EcoRV EcoT22I FbaI FseI HindIII
KasI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1325 GGTACC G^GTACC
AccIII 536 TCCGGA T^CCGGA
AcsI 2158 AAATTC [GA]^AATT[CT]
AhdI 659 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2158 AAATTC [GA]^AATT[CT]
Asp700I 447 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1325 GGTACC G^GTACC
AspEI 659 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 256 GAGCAC G[AT]GC[AT]^C
AvrII 882 CCTAGG C^CTAGG
BanI 1325 GGTACC G^G[CT][GA]CC
BfaI 883 CTAG C^TAG
BglI 637 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1427 AGATCT A^GATCT
BlnI 882 CCTAGG C^CTAGG
BsaI 2564 GAGACC GGTCTC(1/5)
BseAI 536 TCCGGA T^CCGGA
BsiHKAI 256 GAGCAC G[AT]GC[AT]^C
BsmI 1854 GCATTC GAATGC(1/-1)
BspEI 536 TCCGGA T^CCGGA
BsrDI 408 CATTGC GCAATG(2/0)
BssSI 1641 CACGAG CACGAG(-5/-1)
BstEII 1395 GGTGACC G^GT[ACGT]ACC
BstPI 1395 GGTGACC G^GT[ACGT]ACC
BstXI 2301 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1427 AGATCT [GA]^GATC[CT]
EagI 1240 CGGCCG C^GGCCG
Eam1105I 659 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1240 CGGCCG C^GGCCG
Eco52I 1240 CGGCCG C^GGCCG
EcoO65I 1395 GGTGACC G^GT[ACGT]ACC
HincII 1414 GTTAAC GT[CT]^[GA]AC
HindII 1414 GTTAAC GT[CT]^[GA]AC
HpaI 1414 GTTAAC GTT^AAC
KpnI 1325 GGTACC GGTAC^C
MaeI 883 CTAG C^TAG
MflI 1427 AGATCT [GA]^GATC[CT]
MroI 536 TCCGGA T^CCGGA
NotI 1239 GCGGCCGC GC^GGCCGC
NspI 2202 GCATGC [GA]CATG^[CT]
PvuI 24 CGATCG CGAT^CG
SfiI 636 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1165 CTCAAG C^T[CT][GA]AG
SphI 2202 GCATGC GCATG^C
StuI 879 AGGCCT AGG^CCT
TfiI 180 GAATC G^A[AT]TC
XcmI 2472 CCATGCCCCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1427 AGATCT [GA]^GATC[CT]
XmnI 447 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 95 GACGTC GACGT^C
355 GACGTC
AlwI 1578 GATCC GGATC(4/5)
1919 GGATC
AocI 1750 CCTGAGG CC^T[ACGT]AGG
1762 CCTAAGG
AvaI 638 CCCGGG C^[CT]CG[GA]G
1295 CCCGGG
BbvCI 334 CCTCAGC CCTCAGC(-5/-2)
1135 GCTGAGG
BlpI 1470 GCTGAGC GC^T[ACGT]AGC
2266 GCTAAGC
Bpu1102I 1470 GCTGAGC GC^T[ACGT]AGC
2266 GCTAAGC
BsmBI 1453 CGTCTC CGTCTC(1/5)
493 GAGACG
BsoBI 638 CCCGGG C^[CT]CG[GA]G
1295 CCCGGG
BsrBI 1125 GAGCGG CCGCTC(-3/-3)
1795 GAGCGG
BstAPI 543 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1539 GCAGATTGTGC
Bsu36I 1750 CCTGAGG CC^T[ACGT]AGG
1762 CCTAAGG
CelII 1470 GCTGAGC GC^T[ACGT]AGC
2266 GCTAAGC
Eco81I 1750 CCTGAGG CC^T[ACGT]AGG
1762 CCTAAGG
KspI 1217 CCGCGG CCGC^GG
1237 CCGCGG
PstI 1441 CTGCAG CTGCA^G
2441 CTGCAG
SacII 1217 CCGCGG CCGC^GG
1237 CCGCGG
SfcI 1441 CTGCAG C^T[GA][CT]AG
2441 CTGCAG
SmaI 638 CCCGGG CCC^GGG
1295 CCCGGG
Tsp509I 2159 AATT ^AATT
2214 AATT
XmaCI 638 CCCGGG C^CCGGG
1295 CCCGGG
XmaI 638 CCCGGG C^CCGGG
1295 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Aor51HI ApaI AvaII AviII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I