Restriction map for
pF1KB0966
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MboI MflI
MluI MroI MspA1I MspI MvnI
NaeI NarI NciI NdeII NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwNI 32 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 483 GAGCAC G[AT]GC[AT]^C
BalI 975 TGGCCA TGG^CCA
BanI 1250 GGCACC G^G[CT][GA]CC
BfaI 7 CTAG C^TAG
BmyI 483 GAGCAC G[AGT]GC[ACT]^C
BsaAI 396 TACGTG [CT]AC^GT[GA]
BseRI 406 GAGGAG GAGGAG(10/8)
BsiHKAI 483 GAGCAC G[AT]GC[AT]^C
BsmAI 252 GAGAC GTCTC(1/5)
Bsp1286I 483 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 828 TGTACA T^GTACA
BspMI 124 GCAGGT ACCTGC(4/8)
BsrDI 785 GCAATG GCAATG(2/0)
BsrGI 828 TGTACA T^GTACA
BstAPI 1037 GCATTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 33 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 565 GACACACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 216 CCATGG C^C[GA][CT]GG
EaeI 975 TGGCCA [CT]^GGCC[GA]
EarI 64 GAAGAG CTCTTC(1/4)
EcoNI 314 CCTTAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 33 AGGCCCT [GA]G^G[ACGT]CC[CT]
HindIII 548 AAGCTT A^AGCTT
Ksp632I 64 GAAGAG CTCTTC(1/4)
MaeI 7 CTAG C^TAG
MaeII 397 ACGT A^CGT
MfeI 341 CAATTG C^AATTG
MluNI 975 TGGCCA TGG^CCA
MscI 975 TGGCCA TGG^CCA
MunI 341 CAATTG C^AATTG
NcoI 216 CCATGG C^CATGG
PshAI 3 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1268 CTGCAG CTGCA^G
SspBI 828 TGTACA T^GTACA
SspI 800 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflII 315 CTTAAG C^TTAAG
1230 CTTAAG
BbsI 471 GAAGAC GAAGAC(2/6)
886 GTCTTC
BfrI 315 CTTAAG C^TTAAG
1230 CTTAAG
BpuAI 471 GAAGAC GAAGAC(2/6)
886 GTCTTC
BsiYI 314 CCTTAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
444 CCAACAAAAGG
BslI 314 CCTTAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
444 CCAACAAAAGG
BsmI 697 GAATGC GAATGC(1/-1)
1042 GAATGC
BspLU11I 105 ACATGT A^CATGT
235 ACATGT
Cfr13I 34 GGCCC G^G[ACGT]CC
166 GGCCC
EcoRI 587 GAATTC G^AATTC
1007 GAATTC
EcoT14I 119 CCAAGG C^C[AT][AT]GG
216 CCATGG
EcoT22I 500 ATGCAT ATGCA^T
520 ATGCAT
FauI 133 CCCGC CCCGC(4/6)
1046 GCGGG
HphI 511 GGTGA GGTGA(8/7)
1102 GGTGA
NdeI 159 CATATG CA^TATG
1196 CATATG
NlaIV 166 GGCCCC GG[ACGT]^[ACGT]CC
1250 GGCACC
NsiI 500 ATGCAT ATGCA^T
520 ATGCAT
Sau96I 34 GGCCC G^G[ACGT]CC
166 GGCCC
SfaNI 499 GATGC GCATC(5/9)
663 GCATC
SmlI 315 CTTAAG C^T[CT][GA]AG
1230 CTTAAG
StyI 119 CCAAGG C^C[AT][AT]GG
216 CCATGG
TfiI 671 GAATC G^A[AT]TC
755 GAATC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I BbvI BpmI BsaJI
BsgI BsrI BssKI BstF5I BstNI
Cac8I DdeI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboII
MnlI MseI MslI MvaI MwoI
NlaIII NspI PleI RsaI ScrFI
SfcI StuI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI