Restriction map for pF1KB3008
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BbvCI       
BciVI       BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspLU11I    BspMI       BsrFI       BssHII      BssSI       
BstEII      BstPI       BstUI       BstXI       CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          623  GTATAC               GT^[AC][GT]AC
AcyI          589  GACGCC               G[GA]^CG[CT]C
AflIII       1963  ACACGT               A^C[GA][CT]GT
AhdI         2094  GACCATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2255  GTGCAC               G^TGCAC
AocI          594  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2255  GTGCAC               G^TGCAC
Asp700I      2118  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2094  GACCATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          160  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1501  GGATCC               G^GATCC
BcgI         1464  CGATGGCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          242  TGATCA               T^GATCA
BsaHI         589  GACGCC               G[GA]^CG[CT]C
BsaI         1764  GAGACC               GGTCTC(1/5)
BsaWI        2034  TCCGGT               [AT]^CCGG[AT]
BseRI        1877  CTCCTC               GAGGAG(10/8)
BsgI         1105  GTGCAG               GTGCAG(16/14)
Bsp1407I     2334  TGTACA               T^GTACA
BspHI         756  TCATGA               T^CATGA
BsrBI        2111  CCGCTC               CCGCTC(-3/-3)
BsrGI        2334  TGTACA               T^GTACA
Bst1107I      623  GTATAC               GTA^TAC
BstAPI       2139  GCAGAACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1709  TTCGAA               TT^CGAA
BstZ17I       623  GTATAC               GTA^TAC
Bsu36I        594  CCTGAGG              CC^T[ACGT]AGG
DraI          387  TTTAAA               TTT^AAA
DsaI         2134  CCACGG               C^C[GA][CT]GG
Eam1105I     2094  GACCATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        594  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       418  ATGCAT               ATGCA^T
FbaI          242  TGATCA               T^GATCA
HapII        2035  CCGG                 C^CGG
HgaI          589  GACGC                GACGC(5/10)
Hin1I         589  GACGCC               G[GA]^CG[CT]C
HpaII        2035  CCGG                 C^CGG
MfeI         1169  CAATTG               C^AATTG
MspI         2035  CCGG                 C^CGG
MunI         1169  CAATTG               C^AATTG
NdeI         1283  CATATG               CA^TATG
NsiI          418  ATGCAT               ATGCA^T
NspV         1709  TTCGAA               TT^CGAA
PflFI         160  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1061  CTGCAG               CTGCA^G
RcaI          756  TCATGA               T^CATGA
ScaI         1140  AGTACT               AGT^ACT
SfuI         1709  TTCGAA               TT^CGAA
SmlI          305  CTCAAG               C^T[CT][GA]AG
SspBI        2334  TGTACA               T^GTACA
Tth111I       160  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         2118  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BalI         1025  TGGCCA               TGG^CCA
             1258  TGGCCA              
BbsI         2190  GAAGAC               GAAGAC(2/6)
             2393  GAAGAC              
BglII         483  AGATCT               A^GATCT
             1353  AGATCT              
BpuAI        2190  GAAGAC               GAAGAC(2/6)
             2393  GAAGAC              
BsaBI         484  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1354  GATCTTTATC          
BsmI          420  GCATTC               GAATGC(1/-1)
             1434  GCATTC              
BsrDI        1301  GCAATG               GCAATG(2/0)
             1695  GCAATG              
CfoI          655  GCGC                 GCG^C
             1401  GCGC                
EaeI         1025  TGGCCA               [CT]^GGCC[GA]
             1258  TGGCCA              
EarI          493  CTCTTC               CTCTTC(1/4)
              576  GAAGAG              
FauI          791  CCCGC                CCCGC(4/6)
              954  GCGGG               
HhaI          655  GCGC                 GCG^C
             1401  GCGC                
HinP1I        655  GCGC                 G^CGC
             1401  GCGC                
Ksp632I       493  CTCTTC               CTCTTC(1/4)
              576  GAAGAG              
MamI          484  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1354  GATCTTTATC          
MluNI        1025  TGGCCA               TGG^CCA
             1258  TGGCCA              
MscI         1025  TGGCCA               TGG^CCA
             1258  TGGCCA              
MspA1I       1210  CAGCTG               C[AC]G^C[GT]G
             1850  CAGCTG              
PpuMI        1119  AGGTCCT              [GA]G^G[AT]CC[CT]
             1946  AGGTCCC             
PvuII        1210  CAGCTG               CAG^CTG
             1850  CAGCTG              
SacI          641  GAGCTC               GAGCT^C
             1645  GAGCTC              
SfcI         1061  CTGCAG               C^T[GA][CT]AG
             2319  CTATAG              
TaqI          693  TCGA                 T^CGA
             1710  TCGA                
TthHB8I       693  TCGA                 T^CGA
             1710  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
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