################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AvaI AviII AvrII BanI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrFI BssHII BssSI BstEII BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 623 GTATAC GT^[AC][GT]AC AcyI 589 GACGCC G[GA]^CG[CT]C AflIII 1963 ACACGT A^C[GA][CT]GT AhdI 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2255 GTGCAC G^TGCAC AocI 594 CCTGAGG CC^T[ACGT]AGG ApaLI 2255 GTGCAC G^TGCAC Asp700I 2118 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1501 GGATCC G^GATCC BcgI 1464 CGATGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 242 TGATCA T^GATCA BsaHI 589 GACGCC G[GA]^CG[CT]C BsaI 1764 GAGACC GGTCTC(1/5) BsaWI 2034 TCCGGT [AT]^CCGG[AT] BseRI 1877 CTCCTC GAGGAG(10/8) BsgI 1105 GTGCAG GTGCAG(16/14) Bsp1407I 2334 TGTACA T^GTACA BspHI 756 TCATGA T^CATGA BsrBI 2111 CCGCTC CCGCTC(-3/-3) BsrGI 2334 TGTACA T^GTACA Bst1107I 623 GTATAC GTA^TAC BstAPI 2139 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1709 TTCGAA TT^CGAA BstZ17I 623 GTATAC GTA^TAC Bsu36I 594 CCTGAGG CC^T[ACGT]AGG DraI 387 TTTAAA TTT^AAA DsaI 2134 CCACGG C^C[GA][CT]GG Eam1105I 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 594 CCTGAGG CC^T[ACGT]AGG EcoT22I 418 ATGCAT ATGCA^T FbaI 242 TGATCA T^GATCA HapII 2035 CCGG C^CGG HgaI 589 GACGC GACGC(5/10) Hin1I 589 GACGCC G[GA]^CG[CT]C HpaII 2035 CCGG C^CGG MfeI 1169 CAATTG C^AATTG MspI 2035 CCGG C^CGG MunI 1169 CAATTG C^AATTG NdeI 1283 CATATG CA^TATG NsiI 418 ATGCAT ATGCA^T NspV 1709 TTCGAA TT^CGAA PflFI 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1061 CTGCAG CTGCA^G RcaI 756 TCATGA T^CATGA ScaI 1140 AGTACT AGT^ACT SfuI 1709 TTCGAA TT^CGAA SmlI 305 CTCAAG C^T[CT][GA]AG SspBI 2334 TGTACA T^GTACA Tth111I 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2118 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BalI 1025 TGGCCA TGG^CCA 1258 TGGCCA BbsI 2190 GAAGAC GAAGAC(2/6) 2393 GAAGAC BglII 483 AGATCT A^GATCT 1353 AGATCT BpuAI 2190 GAAGAC GAAGAC(2/6) 2393 GAAGAC BsaBI 484 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1354 GATCTTTATC BsmI 420 GCATTC GAATGC(1/-1) 1434 GCATTC BsrDI 1301 GCAATG GCAATG(2/0) 1695 GCAATG CfoI 655 GCGC GCG^C 1401 GCGC EaeI 1025 TGGCCA [CT]^GGCC[GA] 1258 TGGCCA EarI 493 CTCTTC CTCTTC(1/4) 576 GAAGAG FauI 791 CCCGC CCCGC(4/6) 954 GCGGG HhaI 655 GCGC GCG^C 1401 GCGC HinP1I 655 GCGC G^CGC 1401 GCGC Ksp632I 493 CTCTTC CTCTTC(1/4) 576 GAAGAG MamI 484 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1354 GATCTTTATC MluNI 1025 TGGCCA TGG^CCA 1258 TGGCCA MscI 1025 TGGCCA TGG^CCA 1258 TGGCCA MspA1I 1210 CAGCTG C[AC]G^C[GT]G 1850 CAGCTG PpuMI 1119 AGGTCCT [GA]G^G[AT]CC[CT] 1946 AGGTCCC PvuII 1210 CAGCTG CAG^CTG 1850 CAGCTG SacI 641 GAGCTC GAGCT^C 1645 GAGCTC SfcI 1061 CTGCAG C^T[GA][CT]AG 2319 CTATAG TaqI 693 TCGA T^CGA 1710 TCGA TthHB8I 693 TCGA T^CGA 1710 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII