Restriction map for
pF1KB3008
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrFI BssHII BssSI
BstEII BstPI BstUI BstXI CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 623 GTATAC GT^[AC][GT]AC
AcyI 589 GACGCC G[GA]^CG[CT]C
AflIII 1963 ACACGT A^C[GA][CT]GT
AhdI 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2255 GTGCAC G^TGCAC
AocI 594 CCTGAGG CC^T[ACGT]AGG
ApaLI 2255 GTGCAC G^TGCAC
Asp700I 2118 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1501 GGATCC G^GATCC
BcgI 1464 CGATGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 242 TGATCA T^GATCA
BsaHI 589 GACGCC G[GA]^CG[CT]C
BsaI 1764 GAGACC GGTCTC(1/5)
BsaWI 2034 TCCGGT [AT]^CCGG[AT]
BseRI 1877 CTCCTC GAGGAG(10/8)
BsgI 1105 GTGCAG GTGCAG(16/14)
Bsp1407I 2334 TGTACA T^GTACA
BspHI 756 TCATGA T^CATGA
BsrBI 2111 CCGCTC CCGCTC(-3/-3)
BsrGI 2334 TGTACA T^GTACA
Bst1107I 623 GTATAC GTA^TAC
BstAPI 2139 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1709 TTCGAA TT^CGAA
BstZ17I 623 GTATAC GTA^TAC
Bsu36I 594 CCTGAGG CC^T[ACGT]AGG
DraI 387 TTTAAA TTT^AAA
DsaI 2134 CCACGG C^C[GA][CT]GG
Eam1105I 2094 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 594 CCTGAGG CC^T[ACGT]AGG
EcoT22I 418 ATGCAT ATGCA^T
FbaI 242 TGATCA T^GATCA
HapII 2035 CCGG C^CGG
HgaI 589 GACGC GACGC(5/10)
Hin1I 589 GACGCC G[GA]^CG[CT]C
HpaII 2035 CCGG C^CGG
MfeI 1169 CAATTG C^AATTG
MspI 2035 CCGG C^CGG
MunI 1169 CAATTG C^AATTG
NdeI 1283 CATATG CA^TATG
NsiI 418 ATGCAT ATGCA^T
NspV 1709 TTCGAA TT^CGAA
PflFI 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1061 CTGCAG CTGCA^G
RcaI 756 TCATGA T^CATGA
ScaI 1140 AGTACT AGT^ACT
SfuI 1709 TTCGAA TT^CGAA
SmlI 305 CTCAAG C^T[CT][GA]AG
SspBI 2334 TGTACA T^GTACA
Tth111I 160 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2118 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 1025 TGGCCA TGG^CCA
1258 TGGCCA
BbsI 2190 GAAGAC GAAGAC(2/6)
2393 GAAGAC
BglII 483 AGATCT A^GATCT
1353 AGATCT
BpuAI 2190 GAAGAC GAAGAC(2/6)
2393 GAAGAC
BsaBI 484 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1354 GATCTTTATC
BsmI 420 GCATTC GAATGC(1/-1)
1434 GCATTC
BsrDI 1301 GCAATG GCAATG(2/0)
1695 GCAATG
CfoI 655 GCGC GCG^C
1401 GCGC
EaeI 1025 TGGCCA [CT]^GGCC[GA]
1258 TGGCCA
EarI 493 CTCTTC CTCTTC(1/4)
576 GAAGAG
FauI 791 CCCGC CCCGC(4/6)
954 GCGGG
HhaI 655 GCGC GCG^C
1401 GCGC
HinP1I 655 GCGC G^CGC
1401 GCGC
Ksp632I 493 CTCTTC CTCTTC(1/4)
576 GAAGAG
MamI 484 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1354 GATCTTTATC
MluNI 1025 TGGCCA TGG^CCA
1258 TGGCCA
MscI 1025 TGGCCA TGG^CCA
1258 TGGCCA
MspA1I 1210 CAGCTG C[AC]G^C[GT]G
1850 CAGCTG
PpuMI 1119 AGGTCCT [GA]G^G[AT]CC[CT]
1946 AGGTCCC
PvuII 1210 CAGCTG CAG^CTG
1850 CAGCTG
SacI 641 GAGCTC GAGCT^C
1645 GAGCTC
SfcI 1061 CTGCAG C^T[GA][CT]AG
2319 CTATAG
TaqI 693 TCGA T^CGA
1710 TCGA
TthHB8I 693 TCGA T^CGA
1710 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII