Restriction map for
pF1KB7305
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AlwNI AocI Aor51HI ApaI
ApoI AscI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrFI BssHII
BssSI Bst1107I BstAPI BstUI BstZ17I
Bsu36I Cac8I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI FbaI FseI FspI HapII
HgaI Hin1I HpaI HpaII KasI
KpnI KspI MaeII MamI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 1586 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 533 GTGCAC G^TGCAC
ApaLI 533 GTGCAC G^TGCAC
AseI 807 ATTAAT AT^TAAT
AsnI 807 ATTAAT AT^TAAT
Asp700I 472 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1586 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2397 CCCGAG C^[CT]CG[GA]G
BalI 134 TGGCCA TGG^CCA
BanI 2123 GGTGCC G^G[CT][GA]CC
BbsI 2341 GAAGAC GAAGAC(2/6)
BciVI 488 GGATAC GTATCC(6/5)
BglII 310 AGATCT A^GATCT
BpmI 2353 CTGGAG CTGGAG(16/14)
BpuAI 2341 GAAGAC GAAGAC(2/6)
BsaI 2356 GAGACC GGTCTC(1/5)
BsaJI 663 CCTTGG C^C[ACGT][ACGT]GG
BsmI 1918 GAATGC GAATGC(1/-1)
BsoBI 2397 CCCGAG C^[CT]CG[GA]G
Bsp1407I 918 TGTACA T^GTACA
BspHI 2112 TCATGA T^CATGA
BsrBI 2389 GAGCGG CCGCTC(-3/-3)
BsrGI 918 TGTACA T^GTACA
BstBI 1802 TTCGAA TT^CGAA
BstEII 3 GGTTACC G^GT[ACGT]ACC
BstPI 3 GGTTACC G^GT[ACGT]ACC
BstYI 310 AGATCT [GA]^GATC[CT]
EaeI 134 TGGCCA [CT]^GGCC[GA]
Eam1105I 1586 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 663 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3 GGTTACC G^GT[ACGT]ACC
EcoRV 1024 GATATC GAT^ATC
EcoT14I 663 CCTTGG C^C[AT][AT]GG
EcoT22I 956 ATGCAT ATGCA^T
FauI 2308 CCCGC CCCGC(4/6)
HaeII 1619 AGCGCC [GA]GCGC^[CT]
HincII 2182 GTCAAC GT[CT]^[GA]AC
HindII 2182 GTCAAC GT[CT]^[GA]AC
MfeI 1004 CAATTG C^AATTG
MflI 310 AGATCT [GA]^GATC[CT]
MluNI 134 TGGCCA TGG^CCA
MscI 134 TGGCCA TGG^CCA
MslI 1533 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1004 CAATTG C^AATTG
NsiI 956 ATGCAT ATGCA^T
NspV 1802 TTCGAA TT^CGAA
PshBI 807 ATTAAT AT^TAAT
RcaI 2112 TCATGA T^CATGA
SfuI 1802 TTCGAA TT^CGAA
SspBI 918 TGTACA T^GTACA
SspI 1507 AATATT AAT^ATT
StyI 663 CCTTGG C^C[AT][AT]GG
Tsp45I 383 GTCAC ^GT[GC]AC
XhoII 310 AGATCT [GA]^GATC[CT]
XmnI 472 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 2309 CCGC CCGC(-3/-1)
2391 GCGG
AlwI 603 GATCC GGATC(4/5)
555 GGATC
AvaII 1401 GGTCC G^G[AT]CC
2304 GGACC
BglI 2126 GCCTAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2135 GCCAGAGGGGC
BlpI 78 GCTGAGC GC^T[ACGT]AGC
2005 GCTGAGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
2005 GCTGAGC
BsgI 1856 CTGCAC GTGCAG(16/14)
1843 GTGCAG
BsmFI 611 GGGAC GGGAC(10/14)
1168 GTCCC
BssKI 255 CCTGG ^CC[ACGT]GG
965 CCTGG
BstNI 255 CCTGG CC^[AT]GG
965 CCTGG
BstXI 137 CCAGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCAGTATATTGG
CelII 78 GCTGAGC GC^T[ACGT]AGC
2005 GCTGAGC
CfoI 1323 GCGC GCG^C
1620 GCGC
DraI 838 TTTAAA TTT^AAA
1314 TTTAAA
EcoRII 255 CCTGG ^CC[AT]GG
965 CCTGG
HhaI 1323 GCGC GCG^C
1620 GCGC
HinP1I 1323 GCGC G^CGC
1620 GCGC
MvaI 255 CCTGG CC^[AT]GG
965 CCTGG
ScrFI 255 CCTGG CC^[ACGT]GG
965 CCTGG
SfcI 1381 CTACAG C^T[GA][CT]AG
1831 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AspHI BanII BbvI
BfaI BmyI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BstF5I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV PleI RsaI SacI
SapI Sau3AI Sau96I SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I