Restriction map for
pF1KB5616
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BbeI BcgI BclI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoRV
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI MamI
MfeI MroI MunI NaeI NarI
NdeI NgoMIV NheI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 481 GGTACC G^GTACC
AccI 2331 GTCTAC GT^[AC][GT]AC
AflII 2011 CTTAAG C^TTAAG
Asp700I 369 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 481 GGTACC G^GTACC
AspI 2062 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1480 GGATCC G^GATCC
BanI 481 GGTACC G^G[CT][GA]CC
BbrPI 652 CACGTG CAC^GTG
BbsI 618 GAAGAC GAAGAC(2/6)
BbvCI 282 CCTCAGC CCTCAGC(-5/-2)
BciVI 1841 GTATCC GTATCC(6/5)
BfrI 2011 CTTAAG C^TTAAG
BpuAI 618 GAAGAC GAAGAC(2/6)
BsmI 1531 GAATGC GAATGC(1/-1)
BspLU11I 569 ACATGT A^CATGT
BsrBI 25 CCGCTC CCGCTC(-3/-3)
BsrFI 1738 ACCGGC [GA]^CCGG[CT]
BstAPI 1631 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 366 TTCGAA TT^CGAA
BstXI 929 CCACTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1480 GGATCC [GA]^GATC[CT]
Cfr10I 1738 ACCGGC [GA]^CCGG[CT]
DrdI 2062 GACTGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2254 GAATTC G^AATTC
EcoT22I 800 ATGCAT ATGCA^T
HgaI 660 GACGC GACGC(5/10)
KpnI 481 GGTACC GGTAC^C
MflI 1480 GGATCC [GA]^GATC[CT]
MluI 1762 ACGCGT A^CGCGT
NsiI 800 ATGCAT ATGCA^T
NspV 366 TTCGAA TT^CGAA
PflFI 2062 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 652 CACGTG CAC^GTG
PmlI 652 CACGTG CAC^GTG
PpuMI 1435 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1560 CTGCAG CTGCA^G
SapI 2125 GAAGAGC GCTCTTC(1/4)
SexAI 671 ACCAGGT A^CC[AT]GGT
SfuI 366 TTCGAA TT^CGAA
SmaI 1541 CCCGGG CCC^GGG
Tth111I 2062 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1480 GGATCC [GA]^GATC[CT]
XmaCI 1541 CCCGGG C^CCGGG
XmaI 1541 CCCGGG C^CCGGG
XmnI 369 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 1481 GATCC GGATC(4/5)
1480 GGATC
ApaI 79 GGGCCC GGGCC^C
2145 GGGCCC
AvaI 260 CCCGAG C^[CT]CG[GA]G
1541 CCCGGG
BalI 491 TGGCCA TGG^CCA
1695 TGGCCA
BfaI 268 CTAG C^TAG
2096 CTAG
BsaAI 652 CACGTG [CT]AC^GT[GA]
2349 CACGTA
BseRI 2227 CTCCTC GAGGAG(10/8)
1501 GAGGAG
BsoBI 260 CCCGAG C^[CT]CG[GA]G
1541 CCCGGG
BstEII 675 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
BstPI 675 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
EarI 1168 GAAGAG CTCTTC(1/4)
2125 GAAGAG
EcoO65I 675 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
HaeII 45 GGCGCT [GA]GCGC^[CT]
54 GGCGCT
Ksp632I 1168 GAAGAG CTCTTC(1/4)
2125 GAAGAG
KspI 13 CCGCGG CCGC^GG
1809 CCGCGG
MaeI 268 CTAG C^TAG
2096 CTAG
MluNI 491 TGGCCA TGG^CCA
1695 TGGCCA
MscI 491 TGGCCA TGG^CCA
1695 TGGCCA
MseI 1907 TTAA T^TAA
2012 TTAA
NotI 9 GCGGCCGC GC^GGCCGC
1805 GCGGCCGC
NspI 569 ACATGT [GA]CATG^[CT]
1778 GCATGT
PvuII 1704 CAGCTG CAG^CTG
1830 CAGCTG
SacI 108 GAGCTC GAGCT^C
1045 GAGCTC
SacII 13 CCGCGG CCGC^GG
1809 CCGCGG
Tru9I 1907 TTAA T^TAA
2012 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI