Restriction map for
pF1KE0049
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BbeI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 971 CCGC CCGC(-3/-1)
AflIII 1995 ACATGT A^C[GA][CT]GT
AlwNI 959 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 2169 GGATCC G^GATCC
BanI 652 GGTGCC G^G[CT][GA]CC
BanII 2010 GGGCTC G[GA]GC[CT]^C
BbrPI 1319 CACGTG CAC^GTG
BbsI 2092 GAAGAC GAAGAC(2/6)
BbvCI 877 GCTGAGG CCTCAGC(-5/-2)
BclI 567 TGATCA T^GATCA
BpuAI 2092 GAAGAC GAAGAC(2/6)
BsaAI 1319 CACGTG [CT]AC^GT[GA]
BsgI 61 CTGCAC GTGCAG(16/14)
BsmI 1728 GAATGC GAATGC(1/-1)
BspLU11I 1995 ACATGT A^CATGT
BssSI 1624 CACGAG CACGAG(-5/-1)
BstAPI 1009 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 599 TTCGAA TT^CGAA
BstF5I 437 GGATG GGATG(2/0)
BstXI 308 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1975 TTTAAA TTT^AAA
DsaI 229 CCATGG C^C[GA][CT]GG
EcoNI 1273 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 567 TGATCA T^GATCA
FokI 437 GGATG GGATG(9/13)
HindIII 344 AAGCTT A^AGCTT
HpaI 1681 GTTAAC GTT^AAC
MaeII 1320 ACGT A^CGT
MspA1I 1754 CAGCTG C[AC]G^C[GT]G
NcoI 229 CCATGG C^CATGG
NspV 599 TTCGAA TT^CGAA
PmaCI 1319 CACGTG CAC^GTG
PmlI 1319 CACGTG CAC^GTG
PpuMI 535 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1754 CAGCTG CAG^CTG
SfuI 599 TTCGAA TT^CGAA
SmlI 608 CTCAAG C^T[CT][GA]AG
StuI 1226 AGGCCT AGG^CCT
TaqI 600 TCGA T^CGA
TthHB8I 600 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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Asp700I 811 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1285 GAAGAAATTC
AspI 469 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
757 GACATTGTC
BsaI 1265 GAGACC GGTCTC(1/5)
2077 GGTCTC
BsmFI 1950 GGGAC GGGAC(10/14)
537 GTCCC
DraII 502 AGGCCCC [GA]G^G[ACGT]CC[CT]
535 AGGTCCC
EcoO109I 502 AGGCCCC [GA]G^G[ACGT]CC[CT]
535 AGGTCCC
HincII 1330 GTTGAC GT[CT]^[GA]AC
1681 GTTAAC
HindII 1330 GTTGAC GT[CT]^[GA]AC
1681 GTTAAC
MfeI 1217 CAATTG C^AATTG
2051 CAATTG
MunI 1217 CAATTG C^AATTG
2051 CAATTG
PflFI 469 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
757 GACATTGTC
SapI 80 GAAGAGC GCTCTTC(1/4)
1855 GAAGAGC
SphI 822 GCATGC GCATG^C
1238 GCATGC
Tsp45I 751 GTCAC ^GT[GC]AC
869 GTGAC
Tth111I 469 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
757 GACATTGTC
XcmI 1052 CCAATGGTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1235 CCAGCATGCCAGTGG
XmnI 811 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1285 GAAGAAATTC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BalI BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII