Restriction map for
pF1KB5080
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BspMI BssHII BssSI
BstAPI BstEII BstPI BstUI Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
StuI SwaI Tsp45I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AgeI 50 ACCGGT A^CCGGT
AlwI 1168 GATCC GGATC(4/5)
AviII 194 TGCGCA TGC^GCA
BanII 1920 GAGCTC G[GA]GC[CT]^C
BbsI 1628 GAAGAC GAAGAC(2/6)
BcnI 621 CCGGG CC^[GC]GG
BpuAI 1628 GAAGAC GAAGAC(2/6)
BsaI 1540 GGTCTC GGTCTC(1/5)
BsaWI 50 ACCGGT [AT]^CCGG[AT]
Bsp1407I 216 TGTACA T^GTACA
BspHI 605 TCATGA T^CATGA
BsrBI 299 CCGCTC CCGCTC(-3/-3)
BsrFI 50 ACCGGT [GA]^CCGG[CT]
BsrGI 216 TGTACA T^GTACA
Bst1107I 1644 GTATAC GTA^TAC
BstBI 1144 TTCGAA TT^CGAA
BstYI 1167 AGATCC [GA]^GATC[CT]
BstZ17I 1644 GTATAC GTA^TAC
Cfr10I 50 ACCGGT [GA]^CCGG[CT]
DrdI 1888 GACATTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 792 CCATGG C^C[GA][CT]GG
EarI 1580 CTCTTC CTCTTC(1/4)
EcoNI 1189 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 259 ATGCAT ATGCA^T
FspI 194 TGCGCA TGC^GCA
HaeII 870 AGCGCC [GA]GCGC^[CT]
Ksp632I 1580 CTCTTC CTCTTC(1/4)
MflI 1167 AGATCC [GA]^GATC[CT]
NciI 621 CCGGG CC^[GC]GG
NcoI 792 CCATGG C^CATGG
NsiI 259 ATGCAT ATGCA^T
NspV 1144 TTCGAA TT^CGAA
PaeR7I 1223 CTCGAG C^TCGAG
PinAI 50 ACCGGT A^CCGGT
PpuMI 152 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 605 TCATGA T^CATGA
SacI 1920 GAGCTC GAGCT^C
SbfI 182 CCTGCAGG CCTGCA^GG
SfuI 1144 TTCGAA TT^CGAA
SphI 1724 GCATGC GCATG^C
Sse8387I 182 CCTGCAGG CCTGCA^GG
SspBI 216 TGTACA T^GTACA
SspI 1375 AATATT AAT^ATT
XcmI 497 CCAAATCCATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1223 CTCGAG C^TCGAG
XhoII 1167 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 748 GTAGAC GT^[AC][GT]AC
1644 GTATAC
Alw44I 1207 GTGCAC G^TGCAC
1450 GTGCAC
ApaLI 1207 GTGCAC G^TGCAC
1450 GTGCAC
Asp700I 708 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2085 GAAAGTGTTC
AvaI 66 CCCGAG C^[CT]CG[GA]G
1223 CTCGAG
AvaII 153 GGTCC G^G[AT]CC
552 GGACC
BalI 347 TGGCCA TGG^CCA
1422 TGGCCA
BsmBI 61 CGTCTC CGTCTC(1/5)
1268 GAGACG
BsmFI 955 GGGAC GGGAC(10/14)
1897 GTCCC
BsoBI 66 CCCGAG C^[CT]CG[GA]G
1223 CTCGAG
BsrDI 159 CATTGC GCAATG(2/0)
797 GCAATG
CfoI 195 GCGC GCG^C
871 GCGC
EaeI 347 TGGCCA [CT]^GGCC[GA]
1422 TGGCCA
FauI 298 CCCGC CCCGC(4/6)
2008 GCGGG
HapII 51 CCGG C^CGG
621 CCGG
HhaI 195 GCGC GCG^C
871 GCGC
HinP1I 195 GCGC G^CGC
871 GCGC
HpaII 51 CCGG C^CGG
621 CCGG
MaeII 1448 ACGT A^CGT
1699 ACGT
MaeIII 1036 GTTAC ^GT[ACGT]AC
1696 GTAAC
MluNI 347 TGGCCA TGG^CCA
1422 TGGCCA
MscI 347 TGGCCA TGG^CCA
1422 TGGCCA
MspI 51 CCGG C^CGG
621 CCGG
PflMI 868 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2065 CCAAAGGATGG
SmlI 1223 CTCGAG C^T[CT][GA]AG
1549 CTCAAG
Van91I 868 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2065 CCAAAGGATGG
XmnI 708 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2085 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BbvI BclI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I