Restriction map for
pF1KB3072
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BbrPI BbsI
BclI BfrI BglII BlnI BpuAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII KasI KpnI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspBI SspI SwaI
TaqI TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 1559 GGCGTC G[GA]^CG[CT]C
AgeI 1796 ACCGGT A^CCGGT
Alw44I 1189 GTGCAC G^TGCAC
ApaLI 1189 GTGCAC G^TGCAC
Asp700I 1284 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 136 CCTCAGC CCTCAGC(-5/-2)
BcgI 1496 GCACCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1582 TACGTG [CT]AC^GT[GA]
BsaHI 1559 GGCGTC G[GA]^CG[CT]C
BsaWI 1796 ACCGGT [AT]^CCGG[AT]
BssSI 100 CACGAG CACGAG(-5/-1)
DraIII 1771 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1737 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1559 GGCGTC G[GA]^CG[CT]C
HpaI 664 GTTAAC GTT^AAC
KspI 1483 CCGCGG CCGC^GG
MaeII 1583 ACGT A^CGT
MseI 665 TTAA T^TAA
NaeI 1442 GCCGGC GCC^GGC
NgoMIV 1442 GCCGGC G^CCGGC
PflFI 1042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1796 ACCGGT A^CCGGT
SacII 1483 CCGCGG CCGC^GG
SapI 1833 GAAGAGC GCTCTTC(1/4)
SbfI 855 CCTGCAGG CCTGCA^GG
SmaI 155 CCCGGG CCC^GGG
SphI 1652 GCATGC GCATG^C
Sse8387I 855 CCTGCAGG CCTGCA^GG
StuI 826 AGGCCT AGG^CCT
Tru9I 665 TTAA T^TAA
Tth111I 1042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1163 CCAAGGAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
XmnI 1284 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 201 GAATTT [GA]^AATT[CT]
318 AAATTT
AhdI 991 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACCCGGCGTC
ApoI 201 GAATTT [GA]^AATT[CT]
318 AAATTT
AspEI 991 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACCCGGCGTC
BalI 839 TGGCCA TGG^CCA
1602 TGGCCA
BanI 49 GGCACC G^G[CT][GA]CC
333 GGTGCC
BciVI 780 GTATCC GTATCC(6/5)
1461 GTATCC
BfaI 382 CTAG C^TAG
433 CTAG
BglI 25 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
971 GCCATGGAGGC
BsaI 146 GAGACC GGTCTC(1/5)
261 GAGACC
BsmAI 146 GAGAC GTCTC(1/5)
261 GAGAC
BspHI 461 TCATGA T^CATGA
680 TCATGA
BsrBI 1168 GAGCGG CCGCTC(-3/-3)
1312 GAGCGG
BsrFI 1442 GCCGGC [GA]^CCGG[CT]
1796 ACCGGT
BstAPI 789 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1074 GCAGCTGCTGC
Cfr10I 1442 GCCGGC [GA]^CCGG[CT]
1796 ACCGGT
EaeI 839 TGGCCA [CT]^GGCC[GA]
1602 TGGCCA
Eam1105I 991 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACCCGGCGTC
HgaI 1042 GACGC GACGC(5/10)
1560 GCGTC
HincII 664 GTTAAC GT[CT]^[GA]AC
1723 GTCAAC
HindII 664 GTTAAC GT[CT]^[GA]AC
1723 GTCAAC
MaeI 382 CTAG C^TAG
433 CTAG
MluNI 839 TGGCCA TGG^CCA
1602 TGGCCA
MscI 839 TGGCCA TGG^CCA
1602 TGGCCA
MslI 51 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1710 CATGCCTGTG
PflMI 359 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGCTTCTGG
RcaI 461 TCATGA T^CATGA
680 TCATGA
SacI 445 GAGCTC GAGCT^C
1617 GAGCTC
TfiI 465 GATTC G^A[AT]TC
615 GATTC
Tsp509I 202 AATT ^AATT
319 AATT
Van91I 359 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XhoII