Restriction map for
pF1KE0107
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 379 GACGTC GACGT^C
AccII 769 CGCG CG^CG
AfaI 528 GTAC GT^AC
AspHI 237 GAGCTC G[AT]GC[AT]^C
AvaI 737 CCCGGG C^[CT]CG[GA]G
BanII 237 GAGCTC G[GA]GC[CT]^C
BbsI 159 GAAGAC GAAGAC(2/6)
BcgI 795 CGATGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 691 GGATAC GTATCC(6/5)
BglI 62 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 76 CTGGAG CTGGAG(16/14)
BpuAI 159 GAAGAC GAAGAC(2/6)
BsaAI 573 TACGTG [CT]AC^GT[GA]
BsaI 741 GGTCTC GGTCTC(1/5)
BsaWI 524 TCCGGT [AT]^CCGG[AT]
BsiHKAI 237 GAGCTC G[AT]GC[AT]^C
BsmBI 28 GAGACG CGTCTC(1/5)
BsoBI 737 CCCGGG C^[CT]CG[GA]G
BspHI 155 TCATGA T^CATGA
BspLU11I 316 ACATGT A^CATGT
BspMI 590 ACCTGC ACCTGC(4/8)
BssSI 19 CACGAG CACGAG(-5/-1)
BstAPI 345 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 769 CGCG CG^CG
BstXI 363 CCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 290 AGATCC [GA]^GATC[CT]
CfoI 468 GCGC GCG^C
DrdI 30 GACGCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 474 CGGCCA [CT]^GGCC[GA]
EarI 45 GAAGAG CTCTTC(1/4)
EcoT14I 452 CCATGG C^C[AT][AT]GG
HhaI 468 GCGC GCG^C
HinP1I 468 GCGC G^CGC
Ksp632I 45 GAAGAG CTCTTC(1/4)
MflI 290 AGATCC [GA]^GATC[CT]
MspA1I 346 CAGCTG C[AC]G^C[GT]G
MvnI 769 CGCG CG^CG
NcoI 452 CCATGG C^CATGG
PflMI 452 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 839 GAGTC GAGTC(4/5)
PstI 352 CTGCAG CTGCA^G
PvuII 346 CAGCTG CAG^CTG
RcaI 155 TCATGA T^CATGA
RsaI 528 GTAC GT^AC
SacI 237 GAGCTC GAGCT^C
SapI 45 GAAGAGC GCTCTTC(1/4)
SexAI 116 ACCAGGT A^CC[AT]GGT
SfcI 352 CTGCAG C^T[GA][CT]AG
SmaI 737 CCCGGG CCC^GGG
SmlI 64 CTCAAG C^T[CT][GA]AG
StuI 488 AGGCCT AGG^CCT
StyI 452 CCATGG C^C[AT][AT]GG
Van91I 452 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 290 AGATCC [GA]^GATC[CT]
XmaCI 737 CCCGGG C^CCGGG
XmaI 737 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 36 GGCGTC G[GA]^CG[CT]C
379 GACGTC
AflIII 316 ACATGT A^C[GA][CT]GT
574 ACGTGT
BcnI 737 CCCGG CC^[GC]GG
738 CCGGG
BmyI 237 GAGCTC G[AGT]GC[ACT]^C
320 GTGCCC
BsaHI 36 GGCGTC G[GA]^CG[CT]C
379 GACGTC
BsmAI 28 GAGAC GTCTC(1/5)
742 GTCTC
Bsp1286I 237 GAGCTC G[AGT]GC[ACT]^C
320 GTGCCC
DraIII 100 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
115 CACCAGGTG
Hin1I 36 GGCGTC G[GA]^CG[CT]C
379 GACGTC
HphI 258 GGTGA GGTGA(8/7)
278 TCACC
MaeIII 243 GTCAC ^GT[ACGT]AC
440 GTGAC
MboII 45 GAAGA GAAGA(8/7)
159 GAAGA
MslI 364 CACGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
420 CACCTTCGTG
NciI 737 CCCGG CC^[GC]GG
738 CCGGG
NspI 316 ACATGT [GA]CATG^[CT]
464 ACATGC
PpuMI 169 AGGACCC [GA]G^G[AT]CC[CT]
733 GGGTCCC
TfiI 271 GATTC G^A[AT]TC
505 GAATC
Tsp45I 243 GTCAC ^GT[GC]AC
440 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsrBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII ItaI MaeII
MboI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI