Restriction map for
pF1KE0062
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstPI BstUI BstXI
Bsu36I CelII Cfr10I CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 815 GACGCC G[GA]^CG[CT]C
Asp700I 1264 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 209 GGCACC G^G[CT][GA]CC
BbsI 367 GTCTTC GAAGAC(2/6)
BcgI 1099 CGAGTGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 471 GTATCC GTATCC(6/5)
BpmI 179 CTGGAG CTGGAG(16/14)
BpuAI 367 GTCTTC GAAGAC(2/6)
BsaBI 424 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 815 GACGCC G[GA]^CG[CT]C
BsaWI 200 TCCGGT [AT]^CCGG[AT]
BspDI 1318 ATCGAT AT^CGAT
Bst1107I 628 GTATAC GTA^TAC
BstBI 1628 TTCGAA TT^CGAA
BstZ17I 628 GTATAC GTA^TAC
CfoI 873 GCGC GCG^C
ClaI 1318 ATCGAT AT^CGAT
DsaI 1085 CCATGG C^C[GA][CT]GG
HgaI 815 GACGC GACGC(5/10)
HhaI 873 GCGC GCG^C
Hin1I 815 GACGCC G[GA]^CG[CT]C
HinP1I 873 GCGC G^CGC
HphI 927 GGTGA GGTGA(8/7)
MaeII 1812 ACGT A^CGT
MamI 424 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1085 CCATGG C^CATGG
NspV 1628 TTCGAA TT^CGAA
PstI 674 CTGCAG CTGCA^G
SacI 1533 GAGCTC GAGCT^C
ScaI 567 AGTACT AGT^ACT
SfcI 674 CTGCAG C^T[GA][CT]AG
SfuI 1628 TTCGAA TT^CGAA
SpeI 1720 ACTAGT A^CTAGT
StuI 1571 AGGCCT AGG^CCT
XmnI 1264 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccI 628 GTATAC GT^[AC][GT]AC
996 GTCTAC
AcsI 1225 AAATTC [GA]^AATT[CT]
1848 AAATTT
AfaI 568 GTAC GT^AC
1303 GTAC
AlwNI 732 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
850 CAGCAGCTG
ApoI 1225 AAATTC [GA]^AATT[CT]
1848 AAATTT
AvaI 91 CTCGAG C^[CT]CG[GA]G
610 CTCGAG
AvaII 388 GGACC G^G[AT]CC
858 GGTCC
BamHI 37 GGATCC G^GATCC
1203 GGATCC
BanII 619 GGGCTC G[GA]GC[CT]^C
1533 GAGCTC
BcnI 330 CCGGG CC^[GC]GG
1512 CCGGG
BseRI 131 GAGGAG GAGGAG(10/8)
182 GAGGAG
BsoBI 91 CTCGAG C^[CT]CG[GA]G
610 CTCGAG
BspHI 519 TCATGA T^CATGA
1212 TCATGA
BstF5I 198 CATCC GGATG(2/0)
485 GGATG
Cfr13I 388 GGACC G^G[ACGT]CC
858 GGTCC
EarI 1767 CTCTTC CTCTTC(1/4)
84 GAAGAG
EcoRV 913 GATATC GAT^ATC
1634 GATATC
FokI 198 CATCC GGATG(9/13)
485 GGATG
HaeIII 864 GGCC GG^CC
1572 GGCC
Ksp632I 1767 CTCTTC CTCTTC(1/4)
84 GAAGAG
MseI 158 TTAA T^TAA
1159 TTAA
MslI 514 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1357 CATTATTGTG
NciI 330 CCGGG CC^[GC]GG
1512 CCGGG
PaeR7I 91 CTCGAG C^TCGAG
610 CTCGAG
PflMI 849 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1738 CCACTGATTGG
PleI 89 GACTC GAGTC(4/5)
1765 GACTC
RcaI 519 TCATGA T^CATGA
1212 TCATGA
RsaI 568 GTAC GT^AC
1303 GTAC
Sau96I 388 GGACC G^G[ACGT]CC
858 GGTCC
SfaNI 424 GATGC GCATC(5/9)
984 GCATC
SmlI 91 CTCGAG C^T[CT][GA]AG
610 CTCGAG
SphI 437 GCATGC GCATG^C
1341 GCATGC
SspI 554 AATATT AAT^ATT
1186 AATATT
Tru9I 158 TTAA T^TAA
1159 TTAA
Van91I 849 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1738 CCACTGATTGG
XhoI 91 CTCGAG C^TCGAG
610 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AflIII AluI AlwI AspHI BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstNI BstYI Cac8I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HapII HinfI HpaII ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII Sau3AI
ScrFI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII