Restriction map for
pF1KE0095
##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AflII AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BciVI BclI BfrI
BglII BlnI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DraIII EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MfeI
MroI MunI NdeI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1747 GACGTC GACGT^C
AccI 2269 GTAGAC GT^[AC][GT]AC
AcsI 1691 GAATTC [GA]^AATT[CT]
AgeI 3634 ACCGGT A^CCGGT
AhdI 1747 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1452 GTGCAC G^TGCAC
AocI 2134 CCTGAGG CC^T[ACGT]AGG
Aor51HI 692 AGCGCT AGC^GCT
ApaLI 1452 GTGCAC G^TGCAC
ApoI 1691 GAATTC [GA]^AATT[CT]
AspEI 1747 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1198 GCTGAGG CCTCAGC(-5/-2)
BfaI 1771 CTAG C^TAG
BsaWI 3634 ACCGGT [AT]^CCGG[AT]
BsmBI 1566 CGTCTC CGTCTC(1/5)
BspHI 1472 TCATGA T^CATGA
BsrDI 3173 GCAATG GCAATG(2/0)
BssHII 501 GCGCGC G^CGCGC
BssSI 751 CTCGTG CACGAG(-5/-1)
BstYI 1541 AGATCC [GA]^GATC[CT]
Bsu36I 2134 CCTGAGG CC^T[ACGT]AGG
DrdI 2875 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1747 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 692 AGCGCT AGC^GCT
Eco81I 2134 CCTGAGG CC^T[ACGT]AGG
EcoNI 3129 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1691 GAATTC G^AATTC
KspI 150 CCGCGG CCGC^GG
MaeI 1771 CTAG C^TAG
MflI 1541 AGATCC [GA]^GATC[CT]
MluI 2630 ACGCGT A^CGCGT
MseI 1538 TTAA T^TAA
NcoI 1322 CCATGG C^CATGG
NheI 1770 GCTAGC G^CTAGC
NotI 823 GCGGCCGC GC^GGCCGC
PinAI 3634 ACCGGT A^CCGGT
PshAI 328 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1472 TCATGA T^CATGA
SacII 150 CCGCGG CCGC^GG
SmlI 1894 CTCAAG C^T[CT][GA]AG
SnaBI 1532 TACGTA TAC^GTA
StuI 3098 AGGCCT AGG^CCT
Tru9I 1538 TTAA T^TAA
Tsp509I 1692 AATT ^AATT
XhoII 1541 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
ApaI 592 GGGCCC GGGCC^C
3769 GGGCCC
AviII 1034 TGCGCA TGC^GCA
1097 TGCGCA
BbrPI 2562 CACGTG CAC^GTG
2638 CACGTG
BcgI 1705 CGAGTACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1163 GCACCAACATCG
BspLU11I 2555 ACATGT A^CATGT
3668 ACATGT
FspI 1034 TGCGCA TGC^GCA
1097 TGCGCA
PmaCI 2562 CACGTG CAC^GTG
2638 CACGTG
PmlI 2562 CACGTG CAC^GTG
2638 CACGTG
PpuMI 2576 GGGACCT [GA]G^G[AT]CC[CT]
2917 GGGACCT
ScaI 2255 AGTACT AGT^ACT
2723 AGTACT
SexAI 2060 ACCAGGT A^CC[AT]GGT
3674 ACCAGGT
SgrAI 56 CGCCGGCG C[GA]^CCGG[CT]G
3633 CACCGGTG
SphI 1037 GCATGC GCATG^C
2937 GCATGC
TfiI 106 GATTC G^A[AT]TC
1521 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MamI MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI