Restriction map for
pF1KE0094
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AspI AvrII
BbeI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 227 GGTACC G^GTACC
AccI 754 GTCTAC GT^[AC][GT]AC
AgeI 1368 ACCGGT A^CCGGT
ApaI 701 GGGCCC GGGCC^C
Asp718I 227 GGTACC G^GTACC
AspHI 1033 GAGCAC G[AT]GC[AT]^C
AvaI 43 CCCGAG C^[CT]CG[GA]G
AviII 872 TGCGCA TGC^GCA
BbsI 210 GAAGAC GAAGAC(2/6)
BbvCI 817 GCTGAGG CCTCAGC(-5/-2)
BfaI 1186 CTAG C^TAG
BglI 901 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 210 GAAGAC GAAGAC(2/6)
BsgI 1252 CTGCAC GTGCAG(16/14)
BsiEI 24 CGACCG CG[GA][CT]^CG
BsiHKAI 1033 GAGCAC G[AT]GC[AT]^C
BsmBI 673 GAGACG CGTCTC(1/5)
BsoBI 43 CCCGAG C^[CT]CG[GA]G
BspDI 1300 ATCGAT AT^CGAT
BspMI 230 ACCTGC ACCTGC(4/8)
BsrFI 1368 ACCGGT [GA]^CCGG[CT]
BstXI 1214 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1368 ACCGGT [GA]^CCGG[CT]
ClaI 1300 ATCGAT AT^CGAT
DsaI 176 CCATGG C^C[GA][CT]GG
EcoNI 218 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 872 TGCGCA TGC^GCA
HaeII 1390 GGCGCT [GA]GCGC^[CT]
KpnI 227 GGTACC GGTAC^C
MaeI 1186 CTAG C^TAG
NcoI 176 CCATGG C^CATGG
PinAI 1368 ACCGGT A^CCGGT
PstI 1087 CTGCAG CTGCA^G
ScaI 197 AGTACT AGT^ACT
SfcI 1087 CTGCAG C^T[GA][CT]AG
SfiI 900 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1367 CACCGGTG C[GA]^CCGG[CT]G
SphI 1481 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 357 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
399 GAACAGGTTC
BamHI 348 GGATCC G^GATCC
467 GGATCC
BanI 227 GGTACC G^G[CT][GA]CC
1246 GGCACC
BcgI 1494 CGACGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1557 CGAGCTGCCTGC
BcnI 498 CCGGG CC^[GC]GG
750 CCGGG
BsaWI 800 ACCGGA [AT]^CCGG[AT]
1368 ACCGGT
BseRI 784 CTCCTC GAGGAG(10/8)
1198 GAGGAG
BsmAI 308 GAGAC GTCTC(1/5)
673 GAGAC
BsrBI 538 GAGCGG CCGCTC(-3/-3)
1027 GAGCGG
BsrDI 291 CATTGC GCAATG(2/0)
1167 CATTGC
BstAPI 681 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1542 GCACGAGCTGC
HgaI 675 GACGC GACGC(5/10)
449 GCGTC
HincII 544 GTTGAC GT[CT]^[GA]AC
994 GTCAAC
HindII 544 GTTGAC GT[CT]^[GA]AC
994 GTCAAC
MaeIII 1203 GTCAC ^GT[ACGT]AC
1365 GTCAC
NciI 498 CCGGG CC^[GC]GG
750 CCGGG
NspI 1151 ACATGC [GA]CATG^[CT]
1481 GCATGC
PflMI 573 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCAGCTCATGG
PpuMI 38 AGGACCC [GA]G^G[AT]CC[CT]
827 AGGACCT
Tsp45I 1203 GTCAC ^GT[GC]AC
1365 GTCAC
Van91I 573 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCAGCTCATGG
XmnI 357 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
399 GAACAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanII BbvI BglII
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TseI TspRI TthHB8I XcmI
XhoII