Restriction map for
pF1KE0085
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI KpnI MluI MluNI MroI
MscI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI RcaI RsrII
SalI SbfI SexAI SfiI SgrAI
SphI Sse8387I SspBI StuI StyI
SwaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 2710 AACGTT AA^CGTT
AcyI 125 GGCGCC G[GA]^CG[CT]C
AflII 426 CTTAAG C^TTAAG
AlwNI 1817 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 260 GGGCCC GGGCC^C
AseI 2221 ATTAAT AT^TAAT
AsnI 2221 ATTAAT AT^TAAT
AspI 2367 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 125 GGCGCC G^G[CT][GA]CC
BbeI 125 GGCGCC GGCGC^C
BcgI 198 CGAATTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4158 GGATAC GTATCC(6/5)
BfrI 426 CTTAAG C^TTAAG
BsaBI 1864 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 125 GGCGCC G[GA]^CG[CT]C
BsaI 326 GGTCTC GGTCTC(1/5)
BspMI 3780 GCAGGT ACCTGC(4/8)
BstBI 196 TTCGAA TT^CGAA
BstYI 3315 GGATCT [GA]^GATC[CT]
DraII 259 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 187 CCGCGG C^C[GA][CT]GG
EcoO109I 259 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 199 GAATTC G^AATTC
HaeII 125 GGCGCC [GA]GCGC^[CT]
HgaI 172 GCGTC GACGC(5/10)
Hin1I 125 GGCGCC G[GA]^CG[CT]C
HpaI 1984 GTTAAC GTT^AAC
KasI 125 GGCGCC G^GCGCC
KspI 187 CCGCGG CCGC^GG
MamI 1864 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2590 CAATTG C^AATTG
MflI 3315 GGATCT [GA]^GATC[CT]
MslI 1317 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2590 CAATTG C^AATTG
NarI 125 GGCGCC GG^CGCC
NdeI 4030 CATATG CA^TATG
NspV 196 TTCGAA TT^CGAA
PaeR7I 296 CTCGAG C^TCGAG
PflFI 2367 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 951 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2221 ATTAAT AT^TAAT
Psp1406I 2710 AACGTT AA^CGTT
SacI 3370 GAGCTC GAGCT^C
SacII 187 CCGCGG CCGC^GG
SfoI 125 GGCGCC GGC^GCC
SfuI 196 TTCGAA TT^CGAA
SnaBI 1937 TACGTA TAC^GTA
SpeI 2002 ACTAGT A^CTAGT
Tth111I 2367 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 951 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 335 CCAGCGACGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 296 CTCGAG C^TCGAG
XhoII 3315 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 3370 GAGCTC G[AT]GC[AT]^C
3463 GAGCAC
AvaII 1458 GGTCC G^G[AT]CC
2340 GGACC
BsaAI 1937 TACGTA [CT]AC^GT[GA]
3947 TACGTG
BsgI 2900 CTGCAC GTGCAG(16/14)
3784 GTGCAG
BsiHKAI 3370 GAGCTC G[AT]GC[AT]^C
3463 GAGCAC
BsrDI 3720 CATTGC GCAATG(2/0)
3049 GCAATG
BssHII 80 GCGCGC G^CGCGC
308 GCGCGC
BstF5I 552 GGATG GGATG(2/0)
3677 GGATG
BstXI 2054 CCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3743 CCATAGATTTGG
FokI 552 GGATG GGATG(9/13)
3677 GGATG
HaeIII 261 GGCC GG^CC
3741 GGCC
SapI 1090 GAAGAGC GCTCTTC(1/4)
2989 GAAGAGC
SfcI 2719 CTACAG C^T[GA][CT]AG
3268 CTACAG
SspI 1167 AATATT AAT^ATT
2218 AATATT
Tsp45I 158 GTGAC ^GT[GC]AC
745 GTCAC
XbaI 1360 TCTAGA T^CTAGA
2208 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII FauI
Fnu4HI HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmaI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmaCI XmaI XmnI