Restriction map for
pF1KE0083
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BamHI BanI BbeI BbrPI BbvCI
BciVI BfrI BglI BglII BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI HphI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcsI 1520 AAATTT [GA]^AATT[CT]
AflIII 920 ACATGT A^C[GA][CT]GT
AlwNI 1480 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1520 AAATTT [GA]^AATT[CT]
AseI 384 ATTAAT AT^TAAT
AsnI 384 ATTAAT AT^TAAT
Asp700I 731 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 543 CTCGGG C^[CT]CG[GA]G
AvrII 498 CCTAGG C^CTAGG
BalI 776 TGGCCA TGG^CCA
BanII 538 GAGCTC G[GA]GC[CT]^C
BbsI 1489 GAAGAC GAAGAC(2/6)
BcgI 1237 GCAGACTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 306 TGATCA T^GATCA
BcnI 1160 CCGGG CC^[GC]GG
BlnI 498 CCTAGG C^CTAGG
BlpI 843 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 843 GCTTAGC GC^T[ACGT]AGC
BpuAI 1489 GAAGAC GAAGAC(2/6)
BsaBI 979 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 543 CTCGGG C^[CT]CG[GA]G
BspLU11I 920 ACATGT A^CATGT
BspMI 848 GCAGGT ACCTGC(4/8)
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BsrDI 155 GCAATG GCAATG(2/0)
BstAPI 917 GCAACATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1311 CCACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 480 AGATCC [GA]^GATC[CT]
CelII 843 GCTTAGC GC^T[ACGT]AGC
DraI 510 TTTAAA TTT^AAA
DraII 1481 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 776 TGGCCA [CT]^GGCC[GA]
EarI 433 GAAGAG CTCTTC(1/4)
EcoO109I 1481 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 1295 CCCGC CCCGC(4/6)
FbaI 306 TGATCA T^GATCA
HaeII 1277 GGCGCT [GA]GCGC^[CT]
HapII 1160 CCGG C^CGG
HgaI 1337 GCGTC GACGC(5/10)
HincII 255 GTTGAC GT[CT]^[GA]AC
HindII 255 GTTGAC GT[CT]^[GA]AC
HpaII 1160 CCGG C^CGG
Ksp632I 433 GAAGAG CTCTTC(1/4)
MaeII 240 ACGT A^CGT
MamI 979 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 480 AGATCC [GA]^GATC[CT]
MluNI 776 TGGCCA TGG^CCA
MscI 776 TGGCCA TGG^CCA
MspA1I 1296 CCGCTG C[AC]G^C[GT]G
MspI 1160 CCGG C^CGG
NciI 1160 CCGGG CC^[GC]GG
NcoI 1367 CCATGG C^CATGG
NspI 920 ACATGT [GA]CATG^[CT]
PshBI 384 ATTAAT AT^TAAT
PstI 1226 CTGCAG CTGCA^G
SacI 538 GAGCTC GAGCT^C
SapI 433 GAAGAGC GCTCTTC(1/4)
ScaI 48 AGTACT AGT^ACT
SfcI 1226 CTGCAG C^T[GA][CT]AG
StuI 1328 AGGCCT AGG^CCT
XhoII 480 AGATCC [GA]^GATC[CT]
XmnI 731 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 49 GTAC GT^AC
1102 GTAC
AlwI 481 GATCC GGATC(4/5)
1180 GATCC
AspHI 538 GAGCTC G[AT]GC[AT]^C
685 GAGCAC
BpmI 1332 CTCCAG CTGGAG(16/14)
7 CTGGAG
BsgI 888 CTGCAC GTGCAG(16/14)
1547 CTGCAC
BsiHKAI 538 GAGCTC G[AT]GC[AT]^C
685 GAGCAC
BsmFI 1211 GGGAC GGGAC(10/14)
1461 GGGAC
BsmI 697 GAATGC GAATGC(1/-1)
857 GAATGC
CfoI 564 GCGC GCG^C
1278 GCGC
DsaI 71 CCGTGG C^C[GA][CT]GG
1367 CCATGG
HhaI 564 GCGC GCG^C
1278 GCGC
HinP1I 564 GCGC G^CGC
1278 GCGC
MaeIII 359 GTTAC ^GT[ACGT]AC
1445 GTTAC
PleI 190 GACTC GAGTC(4/5)
1035 GACTC
RsaI 49 GTAC GT^AC
1102 GTAC
SspI 100 AATATT AAT^ATT
464 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstBI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspV Sau3AI
Sau96I ScrFI SfaNI SfuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I