Restriction map for
pF1KE0082
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 262 ACATGT A^C[GA][CT]GT
Alw44I 1665 GTGCAC G^TGCAC
ApaLI 1665 GTGCAC G^TGCAC
Asp700I 924 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 38 TGGCCA TGG^CCA
BfaI 1730 CTAG C^TAG
BglII 480 AGATCT A^GATCT
BlpI 298 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 298 GCTGAGC GC^T[ACGT]AGC
BsaI 1905 GAGACC GGTCTC(1/5)
BsmFI 524 GGGAC GGGAC(10/14)
BspHI 1797 TCATGA T^CATGA
BspLU11I 262 ACATGT A^CATGT
BstEII 955 GGTCACC G^GT[ACGT]ACC
BstPI 955 GGTCACC G^GT[ACGT]ACC
BstYI 480 AGATCT [GA]^GATC[CT]
CelII 298 GCTGAGC GC^T[ACGT]AGC
Cfr13I 246 GGCCC G^G[ACGT]CC
DpnII 481 GATC ^GATC
DraI 219 TTTAAA TTT^AAA
DraIII 1668 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 38 TGGCCA [CT]^GGCC[GA]
EcoO65I 955 GGTCACC G^GT[ACGT]ACC
EcoT22I 444 ATGCAT ATGCA^T
MaeI 1730 CTAG C^TAG
MboI 481 GATC ^GATC
MflI 480 AGATCT [GA]^GATC[CT]
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
MwoI 336 GCTTTACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 481 GATC ^GATC
NsiI 444 ATGCAT ATGCA^T
RcaI 1797 TCATGA T^CATGA
SapI 496 GCTCTTC GCTCTTC(1/4)
Sau3AI 481 GATC ^GATC
Sau96I 246 GGCCC G^G[ACGT]CC
SfcI 194 CTGTAG C^T[GA][CT]AG
SphI 446 GCATGC GCATG^C
XhoII 480 AGATCT [GA]^GATC[CT]
XmnI 924 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 619 GAGCAC G[AT]GC[AT]^C
1665 GTGCAC
BanI 171 GGCACC G^G[CT][GA]CC
1712 GGCACC
BmyI 619 GAGCAC G[AGT]GC[ACT]^C
1665 GTGCAC
BsaBI 475 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1540 GATACACATC
BseRI 58 GAGGAG GAGGAG(10/8)
166 GAGGAG
BsiHKAI 619 GAGCAC G[AT]GC[AT]^C
1665 GTGCAC
Bsp1286I 619 GAGCAC G[AGT]GC[ACT]^C
1665 GTGCAC
Bsp1407I 1019 TGTACA T^GTACA
1447 TGTACA
BspMI 92 ACCTGC ACCTGC(4/8)
341 ACCTGC
BsrDI 183 GCAATG GCAATG(2/0)
995 GCAATG
BsrGI 1019 TGTACA T^GTACA
1447 TGTACA
BstF5I 162 GGATG GGATG(2/0)
991 GGATG
Cac8I 225 GCCAGC GC[ACGT]^[ACGT]GC
446 GCATGC
EcoNI 6 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
933 CCTTACACAGG
EcoT14I 158 CCAAGG C^C[AT][AT]GG
895 CCAAGG
FokI 162 GGATG GGATG(9/13)
991 GGATG
HindIII 334 AAGCTT A^AGCTT
842 AAGCTT
MaeII 35 ACGT A^CGT
80 ACGT
MamI 475 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1540 GATACACATC
PleI 1458 GAGTC GAGTC(4/5)
1934 GAGTC
SspBI 1019 TGTACA T^GTACA
1447 TGTACA
StyI 158 CCAAGG C^C[AT][AT]GG
895 CCAAGG
TfiI 475 GATTC G^A[AT]TC
882 GATTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmI BsrI BssKI BstNI
DdeI EarI EcoRI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboII MnlI MseI MslI
MspA1I MvaI NlaIII NlaIV NspI
PvuII RsaI ScrFI SfaNI StuI
Tru9I TseI Tsp45I Tsp509I TspRI