Restriction map for
pF1KE0081
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BfrI BlnI BsaBI BseAI
BsiWI BsmBI BsmI BspEI BssHII
Bst1107I BstBI BstZ17I CpoI Eco47III
EcoRV EcoT22I FseI HaeII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2374 GTCTAC GT^[AC][GT]AC
AflIII 2219 ACATGT A^C[GA][CT]GT
AgeI 793 ACCGGT A^CCGGT
ApaI 1569 GGGCCC GGGCC^C
BanI 24 GGTGCC G^G[CT][GA]CC
BbrPI 1999 CACGTG CAC^GTG
BbvCI 465 GCTGAGG CCTCAGC(-5/-2)
BcgI 3980 GCACCGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 845 TGATCA T^GATCA
BglI 4114 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1999 CACGTG [CT]AC^GT[GA]
BsaWI 793 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1061 TGTACA T^GTACA
BspDI 3901 ATCGAT AT^CGAT
BspLU11I 2219 ACATGT A^CATGT
BsrGI 1061 TGTACA T^GTACA
BstAPI 907 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2064 GGTGACC G^GT[ACGT]ACC
BstPI 2064 GGTGACC G^GT[ACGT]ACC
ClaI 3901 ATCGAT AT^CGAT
DraI 3226 TTTAAA TTT^AAA
DraIII 3502 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 379 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4072 CGGCCG C^GGCCG
EclXI 4072 CGGCCG C^GGCCG
Eco52I 4072 CGGCCG C^GGCCG
EcoO65I 2064 GGTGACC G^GT[ACGT]ACC
EcoRI 1326 GAATTC G^AATTC
FbaI 845 TGATCA T^GATCA
HincII 3241 GTCAAC GT[CT]^[GA]AC
HindII 3241 GTCAAC GT[CT]^[GA]AC
KspI 3495 CCGCGG CCGC^GG
MaeII 2000 ACGT A^CGT
NaeI 2750 GCCGGC GCC^GGC
NdeI 1790 CATATG CA^TATG
NgoMIV 2750 GCCGGC G^CCGGC
PaeR7I 1690 CTCGAG C^TCGAG
PinAI 793 ACCGGT A^CCGGT
PmaCI 1999 CACGTG CAC^GTG
PmlI 1999 CACGTG CAC^GTG
PpuMI 1841 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2052 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3027 GAGCTC GAGCT^C
SacII 3495 CCGCGG CCGC^GG
SexAI 1592 ACCTGGT A^CC[AT]GGT
SgrAI 792 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1462 CCCGGG CCC^GGG
SspBI 1061 TGTACA T^GTACA
XhoI 1690 CTCGAG C^TCGAG
XmaCI 1462 CCCGGG C^CCGGG
XmaI 1462 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 1936 CGCG CG^CG
3496 CGCG
AcyI 1993 GACGCC G[GA]^CG[CT]C
3261 GGCGTC
AhdI 1975 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3637 GACTTGCGGTC
Asp700I 1356 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2760 GAACTGCTTC
AspEI 1975 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3637 GACTTGCGGTC
AviII 853 TGCGCA TGC^GCA
3090 TGCGCA
BciVI 898 GGATAC GTATCC(6/5)
3329 GGATAC
BglII 1958 AGATCT A^GATCT
3737 AGATCT
BlpI 979 GCTCAGC GC^T[ACGT]AGC
2478 GCTGAGC
Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC
2478 GCTGAGC
BsaHI 1993 GACGCC G[GA]^CG[CT]C
3261 GGCGTC
BsrBI 304 CCGCTC CCGCTC(-3/-3)
1885 GAGCGG
BssSI 2661 CACGAG CACGAG(-5/-1)
46 CTCGTG
BstUI 1936 CGCG CG^CG
3496 CGCG
CelII 979 GCTCAGC GC^T[ACGT]AGC
2478 GCTGAGC
DraII 1841 GGGACCT [GA]G^G[ACGT]CC[CT]
1889 GGGGCCT
Eam1105I 1975 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3637 GACTTGCGGTC
EcoNI 3749 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3765 CCTGACCAAGG
EcoO109I 1841 GGGACCT [GA]G^G[ACGT]CC[CT]
1889 GGGGCCT
FspI 853 TGCGCA TGC^GCA
3090 TGCGCA
Hin1I 1993 GACGCC G[GA]^CG[CT]C
3261 GGCGTC
HindIII 318 AAGCTT A^AGCTT
443 AAGCTT
MvnI 1936 CGCG CG^CG
3496 CGCG
SapI 577 GAAGAGC GCTCTTC(1/4)
2694 GCTCTTC
ScaI 840 AGTACT AGT^ACT
2081 AGTACT
SphI 1256 GCATGC GCATG^C
1503 GCATGC
SspI 543 AATATT AAT^ATT
569 AATATT
XmnI 1356 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2760 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII