Restriction map for
pF1KE0078
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# NO CUT ENZYMES ( 86 enzymes) #
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AccIII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MseI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 772 GACGTC GACGT^C
Acc65I 2093 GGTACC G^GTACC
AccI 1177 GTCTAC GT^[AC][GT]AC
Aor51HI 1103 AGCGCT AGC^GCT
Asp718I 2093 GGTACC G^GTACC
AspI 919 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2386 GGCGCC GGCGC^C
BbrPI 1306 CACGTG CAC^GTG
BbsI 1014 GAAGAC GAAGAC(2/6)
BcgI 701 GCATCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1970 AGATCT A^GATCT
BpuAI 1014 GAAGAC GAAGAC(2/6)
Bsp1407I 1289 TGTACA T^GTACA
BspLU11I 1286 ACATGT A^CATGT
BsrBI 1927 CCGCTC CCGCTC(-3/-3)
BsrGI 1289 TGTACA T^GTACA
BstEII 453 GGTCACC G^GT[ACGT]ACC
BstPI 453 GGTCACC G^GT[ACGT]ACC
Eco47III 1103 AGCGCT AGC^GCT
EcoO65I 453 GGTCACC G^GT[ACGT]ACC
KasI 2386 GGCGCC G^GCGCC
KpnI 2093 GGTACC GGTAC^C
NarI 2386 GGCGCC GG^CGCC
NcoI 449 CCATGG C^CATGG
NheI 505 GCTAGC G^CTAGC
PflFI 919 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1306 CACGTG CAC^GTG
PmlI 1306 CACGTG CAC^GTG
PpuMI 1253 AGGACCC [GA]G^G[AT]CC[CT]
SacI 442 GAGCTC GAGCT^C
SbfI 1956 CCTGCAGG CCTGCA^GG
SfoI 2386 GGCGCC GGC^GCC
Sse8387I 1956 CCTGCAGG CCTGCA^GG
SspBI 1289 TGTACA T^GTACA
TaqI 1331 TCGA T^CGA
Tth111I 919 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1331 TCGA T^CGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 772 GACGTC G[GA]^CG[CT]C
2386 GGCGCC
AflIII 1286 ACATGT A^C[GA][CT]GT
1463 ACACGT
AlwI 1452 GATCC GGATC(4/5)
261 GGATC
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
1854 CCTCAGC
BfaI 506 CTAG C^TAG
2194 CTAG
BlpI 519 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
Bpu1102I 519 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
BsaHI 772 GACGTC G[GA]^CG[CT]C
2386 GGCGCC
BssSI 715 CACGAG CACGAG(-5/-1)
994 CACGAG
BstXI 224 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2063 CCATGCTGGTGG
CelII 519 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
DraIII 781 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1405 CACGACGTG
HgaI 1017 GACGC GACGC(5/10)
1859 GCGTC
Hin1I 772 GACGTC G[GA]^CG[CT]C
2386 GGCGCC
HinfI 748 GACTC G^A[ACGT]TC
1981 GAGTC
KspI 590 CCGCGG CCGC^GG
689 CCGCGG
MaeI 506 CTAG C^TAG
2194 CTAG
MslI 225 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2064 CATGCTGGTG
NaeI 656 GCCGGC GCC^GGC
1698 GCCGGC
NgoMIV 656 GCCGGC G^CCGGC
1698 GCCGGC
NspI 1286 ACATGT [GA]CATG^[CT]
2135 ACATGC
PflMI 881 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1404 CCACGACGTGG
PleI 748 GACTC GAGTC(4/5)
1981 GAGTC
SacII 590 CCGCGG CCGC^GG
689 CCGCGG
SfiI 655 GGCCGGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
894 GGCCAGCGAGGCC
SmaI 271 CCCGGG CCC^GGG
527 CCCGGG
StuI 902 AGGCCT AGG^CCT
1799 AGGCCT
Van91I 881 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1404 CCACGACGTGG
XmaCI 271 CCCGGG C^CCGGG
527 CCCGGG
XmaI 271 CCCGGG C^CCGGG
527 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI StyI
TseI Tsp45I TspRI XcmI XhoII