Restriction map for
pF1KE0075
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# NO CUT ENZYMES ( 55 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AscI Asp718I AviII BamHI BciVI
BclI BfrI BseAI BsiWI BspDI
BspEI BssHII BstAPI BstEII BstPI
ClaI EcoO65I EcoRV FbaI FseI
FspI HindIII HpaI KpnI MluI
MroI NheI NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
SacI SalI SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 1306 GACGTC GACGT^C
AccI 568 GTATAC GT^[AC][GT]AC
AhdI 3865 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4141 GTGCAC G^TGCAC
ApaLI 4141 GTGCAC G^TGCAC
AseI 2740 ATTAAT AT^TAAT
AsnI 2740 ATTAAT AT^TAAT
Asp700I 1293 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3865 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 744 CCTAGG C^CTAGG
BbeI 3385 GGCGCC GGCGC^C
BcgI 3113 GCACATTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2051 AGATCT A^GATCT
BlnI 744 CCTAGG C^CTAGG
BlpI 2035 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2035 GCTGAGC GC^T[ACGT]AGC
BsaBI 1795 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 364 GAGACC GGTCTC(1/5)
BsaWI 4253 TCCGGT [AT]^CCGG[AT]
Bsp1407I 575 TGTACA T^GTACA
BspHI 470 TCATGA T^CATGA
BspMI 2129 ACCTGC ACCTGC(4/8)
BsrBI 1703 CCGCTC CCGCTC(-3/-3)
BsrDI 1661 GCAATG GCAATG(2/0)
BsrGI 575 TGTACA T^GTACA
BssSI 3121 CTCGTG CACGAG(-5/-1)
Bst1107I 568 GTATAC GTA^TAC
BstBI 4272 TTCGAA TT^CGAA
BstZ17I 568 GTATAC GTA^TAC
CelII 2035 GCTGAGC GC^T[ACGT]AGC
CpoI 134 CGGACCG CG^G[AT]CCG
DraI 262 TTTAAA TTT^AAA
DraIII 3754 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3865 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 787 GAATTC G^AATTC
EcoT22I 2570 ATGCAT ATGCA^T
HincII 2703 GTTGAC GT[CT]^[GA]AC
HindII 2703 GTTGAC GT[CT]^[GA]AC
KasI 3385 GGCGCC G^GCGCC
KspI 36 CCGCGG CCGC^GG
MamI 1795 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2969 CAATTG C^AATTG
MunI 2969 CAATTG C^AATTG
NaeI 78 GCCGGC GCC^GGC
NarI 3385 GGCGCC GG^CGCC
NdeI 1205 CATATG CA^TATG
NgoMIV 78 GCCGGC G^CCGGC
NsiI 2570 ATGCAT ATGCA^T
NspV 4272 TTCGAA TT^CGAA
PflMI 2522 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2740 ATTAAT AT^TAAT
RcaI 470 TCATGA T^CATGA
RsrII 134 CGGACCG CG^G[AT]CCG
SacII 36 CCGCGG CCGC^GG
SfoI 3385 GGCGCC GGC^GCC
SfuI 4272 TTCGAA TT^CGAA
SspBI 575 TGTACA T^GTACA
Van91I 2522 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1268 CCACCACGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1293 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AocI 1879 CCTCAGG CC^T[ACGT]AGG
3544 CCTGAGG
Aor51HI 519 AGCGCT AGC^GCT
2749 AGCGCT
AspI 2788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3687 GACAGTGTC
BalI 1325 TGGCCA TGG^CCA
3934 TGGCCA
BbrPI 2017 CACGTG CAC^GTG
4237 CACGTG
BbsI 3133 GTCTTC GAAGAC(2/6)
3787 GTCTTC
BfaI 745 CTAG C^TAG
3998 CTAG
BpuAI 3133 GTCTTC GAAGAC(2/6)
3787 GTCTTC
BsaAI 2017 CACGTG [CT]AC^GT[GA]
4237 CACGTG
BsmBI 2315 GAGACG CGTCTC(1/5)
3959 GAGACG
BsmI 4306 GAATGC GAATGC(1/-1)
3987 GCATTC
BstYI 2051 AGATCT [GA]^GATC[CT]
3424 GGATCT
Bsu36I 1879 CCTCAGG CC^T[ACGT]AGG
3544 CCTGAGG
Eco47III 519 AGCGCT AGC^GCT
2749 AGCGCT
Eco81I 1879 CCTCAGG CC^T[ACGT]AGG
3544 CCTGAGG
MaeI 745 CTAG C^TAG
3998 CTAG
MflI 2051 AGATCT [GA]^GATC[CT]
3424 GGATCT
MluNI 1325 TGGCCA TGG^CCA
3934 TGGCCA
MscI 1325 TGGCCA TGG^CCA
3934 TGGCCA
NcoI 1757 CCATGG C^CATGG
4067 CCATGG
PaeR7I 361 CTCGAG C^TCGAG
2167 CTCGAG
PflFI 2788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3687 GACAGTGTC
PmaCI 2017 CACGTG CAC^GTG
4237 CACGTG
PmlI 2017 CACGTG CAC^GTG
4237 CACGTG
PvuII 828 CAGCTG CAG^CTG
2091 CAGCTG
SmaI 69 CCCGGG CCC^GGG
4322 CCCGGG
SmlI 361 CTCGAG C^T[CT][GA]AG
2167 CTCGAG
Tth111I 2788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3687 GACAGTGTC
XhoI 361 CTCGAG C^TCGAG
2167 CTCGAG
XhoII 2051 AGATCT [GA]^GATC[CT]
3424 GGATCT
XmaCI 69 CCCGGG C^CCGGG
4322 CCCGGG
XmaI 69 CCCGGG C^CCGGG
4322 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I