Restriction map for
pF1KE0073
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbsI BclI BfrI
BglII BlnI BpuAI BsaBI BsiWI
Bsp1407I BspDI BsrBI BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI DraI
DrdI EarI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SacI SacII SapI SexAI SfuI
SgrAI SnaBI SspBI SspI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 682 GTCGAC GT^[AC][GT]AC
AccIII 2895 TCCGGA T^CCGGA
AcsI 1113 AAATTC [GA]^AATT[CT]
AflIII 734 ACATGT A^C[GA][CT]GT
Alw44I 262 GTGCAC G^TGCAC
AocI 3566 CCTCAGG CC^T[ACGT]AGG
ApaLI 262 GTGCAC G^TGCAC
ApoI 1113 AAATTC [GA]^AATT[CT]
AspI 2825 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 144 GGATCC G^GATCC
BciVI 1224 GTATCC GTATCC(6/5)
BlpI 1726 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1726 GCTCAGC GC^T[ACGT]AGC
BseAI 2895 TCCGGA T^CCGGA
BsmBI 2977 GAGACG CGTCTC(1/5)
BsmI 3912 GCATTC GAATGC(1/-1)
BspEI 2895 TCCGGA T^CCGGA
BspHI 1667 TCATGA T^CATGA
BspLU11I 734 ACATGT A^CATGT
BstEII 3231 GGTGACC G^GT[ACGT]ACC
BstPI 3231 GGTGACC G^GT[ACGT]ACC
Bsu36I 3566 CCTCAGG CC^T[ACGT]AGG
CelII 1726 GCTCAGC GC^T[ACGT]AGC
CpoI 3282 CGGACCG CG^G[AT]CCG
DraIII 550 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3566 CCTCAGG CC^T[ACGT]AGG
EcoNI 3393 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3231 GGTGACC G^GT[ACGT]ACC
MboII 2550 GAAGA GAAGA(8/7)
MroI 2895 TCCGGA T^CCGGA
PaeR7I 3881 CTCGAG C^TCGAG
PflFI 2825 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2845 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1667 TCATGA T^CATGA
RsrII 3282 CGGACCG CG^G[AT]CCG
SalI 682 GTCGAC G^TCGAC
SbfI 1176 CCTGCAGG CCTGCA^GG
ScaI 2882 AGTACT AGT^ACT
SfiI 1753 GGCCAGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 3881 CTCGAG C^T[CT][GA]AG
SpeI 3204 ACTAGT A^CTAGT
Sse8387I 1176 CCTGCAGG CCTGCA^GG
Tth111I 2825 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3881 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 2883 GTAC GT^AC
3681 GTAC
AhdI 1552 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3169 GACGAGGTGTC
Aor51HI 2167 AGCGCT AGC^GCT
3670 AGCGCT
AspEI 1552 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3169 GACGAGGTGTC
BalI 48 TGGCCA TGG^CCA
1358 TGGCCA
BbrPI 2071 CACGTG CAC^GTG
2854 CACGTG
BbvCI 1042 CCTCAGC CCTCAGC(-5/-2)
3806 CCTCAGC
BsaAI 2071 CACGTG [CT]AC^GT[GA]
2854 CACGTG
BseRI 1295 CTCCTC GAGGAG(10/8)
1997 CTCCTC
BspMI 1922 ACCTGC ACCTGC(4/8)
3399 GCAGGT
BssSI 3150 CACGAG CACGAG(-5/-1)
375 CTCGTG
EagI 2253 CGGCCG C^GGCCG
2817 CGGCCG
Eam1105I 1552 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3169 GACGAGGTGTC
EclXI 2253 CGGCCG C^GGCCG
2817 CGGCCG
Eco47III 2167 AGCGCT AGC^GCT
3670 AGCGCT
Eco52I 2253 CGGCCG C^GGCCG
2817 CGGCCG
HincII 31 GTCAAC GT[CT]^[GA]AC
682 GTCGAC
HindII 31 GTCAAC GT[CT]^[GA]AC
682 GTCGAC
MluNI 48 TGGCCA TGG^CCA
1358 TGGCCA
MscI 48 TGGCCA TGG^CCA
1358 TGGCCA
MslI 186 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1350 CATGAACGTG
PmaCI 2071 CACGTG CAC^GTG
2854 CACGTG
PmlI 2071 CACGTG CAC^GTG
2854 CACGTG
PpuMI 2664 AGGACCC [GA]G^G[AT]CC[CT]
3076 GGGTCCC
RsaI 2883 GTAC GT^AC
3681 GTAC
XcmI 1229 CCATCCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2759 CCAACTACACCCTGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI