Restriction map for
pF1KE0072
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AspHI AvaI AvrII BamHI BanII
BbrPI BbsI BcgI BglI BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BspLU11I BsrBI BssHII BssSI BstEII
BstPI CelII ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI HgaI
HindIII HpaI KspI MamI MluI
MroI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 866 GTATAC GT^[AC][GT]AC
AccII 126 CGCG CG^CG
AcyI 160 GGCGCC G[GA]^CG[CT]C
AflII 2620 CTTAAG C^TTAAG
AhdI 1369 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3253 CCTCAGG CC^T[ACGT]AGG
AseI 2440 ATTAAT AT^TAAT
AsnI 2440 ATTAAT AT^TAAT
AspEI 1369 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2521 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2453 TGCGCA TGC^GCA
BalI 3417 TGGCCA TGG^CCA
BbeI 160 GGCGCC GGCGC^C
BcnI 110 CCGGG CC^[GC]GG
BfrI 2620 CTTAAG C^TTAAG
BsaHI 160 GGCGCC G[GA]^CG[CT]C
BsaI 3197 GAGACC GGTCTC(1/5)
BsaWI 301 ACCGGA [AT]^CCGG[AT]
Bsp1407I 293 TGTACA T^GTACA
BspHI 1299 TCATGA T^CATGA
BsrGI 293 TGTACA T^GTACA
Bst1107I 866 GTATAC GTA^TAC
BstBI 320 TTCGAA TT^CGAA
BstUI 126 CGCG CG^CG
BstXI 2075 CCATAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 866 GTATAC GTA^TAC
Bsu36I 3253 CCTCAGG CC^T[ACGT]AGG
DraII 1611 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2335 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3231 CCATGG C^C[GA][CT]GG
EaeI 3417 TGGCCA [CT]^GGCC[GA]
Eam1105I 1369 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3253 CCTCAGG CC^T[ACGT]AGG
EcoNI 592 CCTGACGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1611 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 72 GCGGG CCCGC(4/6)
FspI 2453 TGCGCA TGC^GCA
Hin1I 160 GGCGCC G[GA]^CG[CT]C
KasI 160 GGCGCC G^GCGCC
MluNI 3417 TGGCCA TGG^CCA
MscI 3417 TGGCCA TGG^CCA
MvnI 126 CGCG CG^CG
NaeI 3441 GCCGGC GCC^GGC
NarI 160 GGCGCC GG^CGCC
NciI 110 CCGGG CC^[GC]GG
NcoI 3231 CCATGG C^CATGG
NgoMIV 3441 GCCGGC G^CCGGC
NspV 320 TTCGAA TT^CGAA
PflFI 2521 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1846 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2440 ATTAAT AT^TAAT
PvuII 2543 CAGCTG CAG^CTG
RcaI 1299 TCATGA T^CATGA
SfoI 160 GGCGCC GGC^GCC
SfuI 320 TTCGAA TT^CGAA
SpeI 2515 ACTAGT A^CTAGT
SphI 1655 GCATGC GCATG^C
SspBI 293 TGTACA T^GTACA
Tth111I 2521 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1846 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2826 TCTAGA T^CTAGA
XcmI 1394 CCATTAAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 1734 GGTACC G^GTACC
3102 GGTACC
Asp718I 1734 GGTACC G^GTACC
3102 GGTACC
AvaII 129 GGTCC G^G[AT]CC
1266 GGACC
BbvCI 3698 CCTCAGC CCTCAGC(-5/-2)
2029 GCTGAGG
BclI 1779 TGATCA T^GATCA
3855 TGATCA
BglII 344 AGATCT A^GATCT
2259 AGATCT
BsrFI 355 ACCGGC [GA]^CCGG[CT]
3441 GCCGGC
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
3441 GCCGGC
EcoRI 1630 GAATTC G^AATTC
2646 GAATTC
FbaI 1779 TGATCA T^GATCA
3855 TGATCA
HaeII 160 GGCGCC [GA]GCGC^[CT]
358 GGCGCT
HincII 2197 GTTGAC GT[CT]^[GA]AC
2782 GTTGAC
HindII 2197 GTTGAC GT[CT]^[GA]AC
2782 GTTGAC
KpnI 1734 GGTACC GGTAC^C
3102 GGTACC
MfeI 2853 CAATTG C^AATTG
3033 CAATTG
MunI 2853 CAATTG C^AATTG
3033 CAATTG
PstI 1409 CTGCAG CTGCA^G
1904 CTGCAG
ScaI 787 AGTACT AGT^ACT
3263 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BanI BbvI
BciVI BfaI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI