Restriction map for
pF1KE0069
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI AocI ApoI AscI
AseI AsnI AspEI AspI AvrII
BbrPI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BseAI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI MaeI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1513 GACGTC GACGT^C
Acc65I 310 GGTACC G^GTACC
AccI 1243 GTCGAC GT^[AC][GT]AC
Alw44I 1595 GTGCAC G^TGCAC
Aor51HI 1418 AGCGCT AGC^GCT
ApaI 1817 GGGCCC GGGCC^C
ApaLI 1595 GTGCAC G^TGCAC
Asp700I 1965 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 310 GGTACC G^GTACC
AviII 41 TGCGCA TGC^GCA
BamHI 1226 GGATCC G^GATCC
BcgI 44 GCACCGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 532 GAGACC GGTCTC(1/5)
BsaWI 641 TCCGGT [AT]^CCGG[AT]
BsgI 1045 CTGCAC GTGCAG(16/14)
BsiEI 450 CGGCCG CG[GA][CT]^CG
BsiWI 1292 CGTACG C^GTACG
BsmI 1695 GAATGC GAATGC(1/-1)
BssSI 913 CACGAG CACGAG(-5/-1)
EagI 450 CGGCCG C^GGCCG
EarI 970 CTCTTC CTCTTC(1/4)
EclXI 450 CGGCCG C^GGCCG
Eco47III 1418 AGCGCT AGC^GCT
Eco52I 450 CGGCCG C^GGCCG
EcoNI 1712 CCTTCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 41 TGCGCA TGC^GCA
HincII 1243 GTCGAC GT[CT]^[GA]AC
HindII 1243 GTCGAC GT[CT]^[GA]AC
KpnI 310 GGTACC GGTAC^C
Ksp632I 970 CTCTTC CTCTTC(1/4)
NaeI 1160 GCCGGC GCC^GGC
NgoMIV 1160 GCCGGC G^CCGGC
NotI 449 GCGGCCGC GC^GGCCGC
PpuMI 1937 AGGACCT [GA]G^G[AT]CC[CT]
SalI 1243 GTCGAC G^TCGAC
SbfI 1447 CCTGCAGG CCTGCA^GG
SexAI 437 ACCTGGT A^CC[AT]GGT
SmlI 1171 CTCAAG C^T[CT][GA]AG
Sse8387I 1447 CCTGCAGG CCTGCA^GG
Tsp509I 1756 AATT ^AATT
XcmI 1935 CCAGGACCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1965 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 954 TGGCCA TGG^CCA
2001 TGGCCA
BlpI 1234 GCTGAGC GC^T[ACGT]AGC
1774 GCTGAGC
BpmI 610 CTGGAG CTGGAG(16/14)
697 CTGGAG
Bpu1102I 1234 GCTGAGC GC^T[ACGT]AGC
1774 GCTGAGC
BsaAI 1354 TACGTG [CT]AC^GT[GA]
2089 TACGTG
BstXI 221 CCATCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1128 CCAGAAGTGTGG
CelII 1234 GCTGAGC GC^T[ACGT]AGC
1774 GCTGAGC
DrdI 181 GACCACTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1507 GACTGTGACGTC
EcoT14I 1397 CCTTGG C^C[AT][AT]GG
1502 CCAAGG
KspI 1668 CCGCGG CCGC^GG
2067 CCGCGG
MluNI 954 TGGCCA TGG^CCA
2001 TGGCCA
MscI 954 TGGCCA TGG^CCA
2001 TGGCCA
PstI 564 CTGCAG CTGCA^G
1448 CTGCAG
PvuII 510 CAGCTG CAG^CTG
1548 CAGCTG
SacI 345 GAGCTC GAGCT^C
1777 GAGCTC
SacII 1668 CCGCGG CCGC^GG
2067 CCGCGG
StuI 122 AGGCCT AGG^CCT
947 AGGCCT
StyI 1397 CCTTGG C^C[AT][AT]GG
1502 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TaqI TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI