Restriction map for
pF1KE0066
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII AccIII AclI AcsI AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BcgI
BfaI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraI DraIII Eam1105I EarI EcoO65I
EcoRI EcoRV EcoT22I FspI HpaI
Ksp632I MaeI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 808 GGTACC G^GTACC
AflII 1126 CTTAAG C^TTAAG
Aor51HI 1814 AGCGCT AGC^GCT
ApaI 165 GGGCCC GGGCC^C
Asp718I 808 GGTACC G^GTACC
BbrPI 2440 CACGTG CAC^GTG
BclI 2472 TGATCA T^GATCA
BfrI 1126 CTTAAG C^TTAAG
BsaAI 2440 CACGTG [CT]AC^GT[GA]
BsaI 1991 GGTCTC GGTCTC(1/5)
BsgI 2104 CTGCAC GTGCAG(16/14)
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsmBI 1730 GAGACG CGTCTC(1/5)
BspLU11I 527 ACATGT A^CATGT
BspMI 461 ACCTGC ACCTGC(4/8)
BsrBI 147 GAGCGG CCGCTC(-3/-3)
Bst1107I 676 GTATAC GTA^TAC
BstZ17I 676 GTATAC GTA^TAC
DsaI 372 CCGCGG C^C[GA][CT]GG
EaeI 113 CGGCCG [CT]^GGCC[GA]
EagI 113 CGGCCG C^GGCCG
EclXI 113 CGGCCG C^GGCCG
Eco47III 1814 AGCGCT AGC^GCT
Eco52I 113 CGGCCG C^GGCCG
EcoNI 1458 CCTCGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2472 TGATCA T^GATCA
FseI 2088 GGCCGGCC GGCCGG^CC
HincII 634 GTCGAC GT[CT]^[GA]AC
HindII 634 GTCGAC GT[CT]^[GA]AC
KpnI 808 GGTACC GGTAC^C
KspI 372 CCGCGG CCGC^GG
NdeI 1981 CATATG CA^TATG
PleI 1199 GAGTC GAGTC(4/5)
PmaCI 2440 CACGTG CAC^GTG
PmlI 2440 CACGTG CAC^GTG
PstI 2343 CTGCAG CTGCA^G
SacI 1285 GAGCTC GAGCT^C
SacII 372 CCGCGG CCGC^GG
SalI 634 GTCGAC G^TCGAC
SgrAI 180 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2337 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 22 enzymes) #
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BbvCI 1773 CCTCAGC CCTCAGC(-5/-2)
2232 GCTGAGG
BciVI 1079 GGATAC GTATCC(6/5)
794 GTATCC
BsrDI 1523 CATTGC GCAATG(2/0)
2355 CATTGC
BsrFI 181 GCCGGC [GA]^CCGG[CT]
2089 GCCGGC
BssHII 401 GCGCGC G^CGCGC
450 GCGCGC
BstXI 798 CCAGATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGCCTCCTGG
BstYI 1101 GGATCT [GA]^GATC[CT]
2282 AGATCC
Cfr10I 181 GCCGGC [GA]^CCGG[CT]
2089 GCCGGC
DrdI 3 GACGCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2380 GACCCCTATGTC
EcoT14I 1400 CCAAGG C^C[AT][AT]GG
2577 CCTTGG
HindIII 2320 AAGCTT A^AGCTT
2478 AAGCTT
MaeIII 1829 GTAAC ^GT[ACGT]AC
2499 GTTAC
MflI 1101 GGATCT [GA]^GATC[CT]
2282 AGATCC
NaeI 181 GCCGGC GCC^GGC
2089 GCCGGC
NgoMIV 181 GCCGGC G^CCGGC
2089 GCCGGC
PpuMI 1709 AGGACCT [GA]G^G[AT]CC[CT]
1766 AGGTCCC
SfaNI 2180 GCATC GCATC(5/9)
2602 GCATC
SfcI 575 CTATAG C^T[GA][CT]AG
2343 CTGCAG
StuI 1900 AGGCCT AGG^CCT
2662 AGGCCT
StyI 1400 CCAAGG C^C[AT][AT]GG
2577 CCTTGG
XcmI 1497 CCAAGCCAACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1602 CCAGATGTCAGTTGG
XhoII 1101 GGATCT [GA]^GATC[CT]
2282 AGATCC
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfoI
SmaI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI