Restriction map for
pF1KE0065
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1173 GTCTAC GT^[AC][GT]AC
AcyI 2462 GGCGCC G[GA]^CG[CT]C
AlwNI 1039 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1723 GAGCAC G[AT]GC[AT]^C
AviII 2516 TGCGCA TGC^GCA
BbeI 2462 GGCGCC GGCGC^C
BbsI 2732 GAAGAC GAAGAC(2/6)
BpuAI 2732 GAAGAC GAAGAC(2/6)
BsaHI 2462 GGCGCC G[GA]^CG[CT]C
BsaWI 740 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1723 GAGCAC G[AT]GC[AT]^C
BspHI 2058 TCATGA T^CATGA
BspLU11I 578 ACATGT A^CATGT
BsrFI 985 GCCGGC [GA]^CCGG[CT]
BssSI 1611 CACGAG CACGAG(-5/-1)
BstEII 2815 GGTGACC G^GT[ACGT]ACC
BstPI 2815 GGTGACC G^GT[ACGT]ACC
Cfr10I 985 GCCGGC [GA]^CCGG[CT]
EcoO65I 2815 GGTGACC G^GT[ACGT]ACC
EcoRI 1572 GAATTC G^AATTC
FspI 2516 TGCGCA TGC^GCA
Hin1I 2462 GGCGCC G[GA]^CG[CT]C
HpaI 754 GTTAAC GTT^AAC
KasI 2462 GGCGCC G^GCGCC
NaeI 985 GCCGGC GCC^GGC
NarI 2462 GGCGCC GG^CGCC
NgoMIV 985 GCCGGC G^CCGGC
NheI 2431 GCTAGC G^CTAGC
NspI 578 ACATGT [GA]CATG^[CT]
RcaI 2058 TCATGA T^CATGA
SfaNI 2950 GATGC GCATC(5/9)
SfoI 2462 GGCGCC GGC^GCC
SmaI 1061 CCCGGG CCC^GGG
XmaCI 1061 CCCGGG C^CCGGG
XmaI 1061 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 578 ACATGT A^C[GA][CT]GT
3000 ACACGT
AhdI 577 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTCCAAGTC
ApaI 1514 GGGCCC GGGCC^C
3029 GGGCCC
Asp700I 229 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
705 GAAGCTTTTC
AspEI 577 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTCCAAGTC
AvaI 1061 CCCGGG C^[CT]CG[GA]G
2467 CCCGAG
BcgI 1613 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1978 GCAAGATCTTCG
BlpI 609 GCTTAGC GC^T[ACGT]AGC
1623 GCTCAGC
Bpu1102I 609 GCTTAGC GC^T[ACGT]AGC
1623 GCTCAGC
BsaI 1996 GAGACC GGTCTC(1/5)
1075 GGTCTC
BsgI 1135 CTGCAC GTGCAG(16/14)
1757 CTGCAC
BsoBI 1061 CCCGGG C^[CT]CG[GA]G
2467 CCCGAG
CelII 609 GCTTAGC GC^T[ACGT]AGC
1623 GCTCAGC
DraII 1513 AGGGCCC [GA]G^G[ACGT]CC[CT]
3028 AGGGCCC
DraIII 1472 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2835 CACTCCGTG
Eam1105I 577 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTCCAAGTC
EarI 2016 CTCTTC CTCTTC(1/4)
2535 CTCTTC
EcoO109I 1513 AGGGCCC [GA]G^G[ACGT]CC[CT]
3028 AGGGCCC
HaeII 553 GGCGCT [GA]GCGC^[CT]
2462 GGCGCC
HgaI 2765 GACGC GACGC(5/10)
2843 GCGTC
HincII 754 GTTAAC GT[CT]^[GA]AC
2196 GTCAAC
HindII 754 GTTAAC GT[CT]^[GA]AC
2196 GTCAAC
Ksp632I 2016 CTCTTC CTCTTC(1/4)
2535 CTCTTC
PflMI 518 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2710 CCAGACGGTGG
PshAI 1069 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1666 GACTCACGTC
PstI 1372 CTGCAG CTGCA^G
2485 CTGCAG
ScaI 994 AGTACT AGT^ACT
2895 AGTACT
SspI 1258 AATATT AAT^ATT
2290 AATATT
StuI 1053 AGGCCT AGG^CCT
2642 AGGCCT
Van91I 518 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2710 CCAGACGGTGG
XmnI 229 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
705 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII