Restriction map for
pF1KE0064
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BamHI BbrPI BbsI BclI
BfrI BglI BglII BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AfaI 460 GTAC GT^AC
AflIII 1116 ACACGT A^C[GA][CT]GT
AlwI 18 GATCC GGATC(4/5)
AocI 520 CCTCAGG CC^T[ACGT]AGG
ApaI 1506 GGGCCC GGGCC^C
Asp700I 1303 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1536 CCTAGG C^CTAGG
BalI 1674 TGGCCA TGG^CCA
BcgI 738 GCACCGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1296 GTATCC GTATCC(6/5)
BlnI 1536 CCTAGG C^CTAGG
BsmBI 957 GAGACG CGTCTC(1/5)
BsmI 439 GAATGC GAATGC(1/-1)
BspHI 1545 TCATGA T^CATGA
BsrDI 1691 GCAATG GCAATG(2/0)
BsrFI 1443 GCCGGC [GA]^CCGG[CT]
BstAPI 1720 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1062 CCAGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 17 AGATCC [GA]^GATC[CT]
Bsu36I 520 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1443 GCCGGC [GA]^CCGG[CT]
DpnII 18 GATC ^GATC
DraIII 537 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 520 CCTCAGG CC^T[ACGT]AGG
EcoNI 1348 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1236 ATGCAT ATGCA^T
HindIII 1140 AAGCTT A^AGCTT
KspI 1330 CCGCGG CCGC^GG
MboI 18 GATC ^GATC
MboII 527 GAAGA GAAGA(8/7)
MflI 17 AGATCC [GA]^GATC[CT]
MluNI 1674 TGGCCA TGG^CCA
MscI 1674 TGGCCA TGG^CCA
NaeI 1443 GCCGGC GCC^GGC
NcoI 1677 CCATGG C^CATGG
NdeII 18 GATC ^GATC
NgoMIV 1443 GCCGGC G^CCGGC
NsiI 1236 ATGCAT ATGCA^T
PflMI 99 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 57 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1165 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1545 TCATGA T^CATGA
RsaI 460 GTAC GT^AC
SacII 1330 CCGCGG CCGC^GG
Sau3AI 18 GATC ^GATC
SbfI 1469 CCTGCAGG CCTGCA^GG
Sse8387I 1469 CCTGCAGG CCTGCA^GG
StuI 631 AGGCCT AGG^CCT
TaqI 980 TCGA T^CGA
TthHB8I 980 TCGA T^CGA
Van91I 99 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1245 TCTAGA T^CTAGA
XcmI 708 CCAGGGCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 17 AGATCC [GA]^GATC[CT]
XmnI 1303 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1259 CGCG CG^CG
1331 CGCG
AlwNI 642 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
918 CAGTCGCTG
AvaII 58 GGTCC G^G[AT]CC
665 GGTCC
BanI 1090 GGCGCC G^G[CT][GA]CC
1446 GGCGCC
BbeI 1090 GGCGCC GGCGC^C
1446 GGCGCC
BbvCI 475 CCTCAGC CCTCAGC(-5/-2)
1107 GCTGAGG
BcnI 668 CCCGG CC^[GC]GG
1461 CCCGG
BsaAI 355 TACGTG [CT]AC^GT[GA]
1117 CACGTA
BsaI 1532 GAGACC GGTCTC(1/5)
1682 GAGACC
BsgI 882 CTGCAC GTGCAG(16/14)
358 GTGCAG
BstUI 1259 CGCG CG^CG
1331 CGCG
DraII 57 GGGTCCC [GA]G^G[ACGT]CC[CT]
1505 GGGGCCC
EaeI 1333 CGGCCA [CT]^GGCC[GA]
1674 TGGCCA
EcoO109I 57 GGGTCCC [GA]G^G[ACGT]CC[CT]
1505 GGGGCCC
HaeII 1090 GGCGCC [GA]GCGC^[CT]
1446 GGCGCC
KasI 1090 GGCGCC G^GCGCC
1446 GGCGCC
MvnI 1259 CGCG CG^CG
1331 CGCG
NarI 1090 GGCGCC GG^CGCC
1446 GGCGCC
NciI 668 CCCGG CC^[GC]GG
1461 CCCGG
PleI 1177 GAGTC GAGTC(4/5)
1549 GAGTC
PvuII 645 CAGCTG CAG^CTG
801 CAGCTG
SfoI 1090 GGCGCC GGC^GCC
1446 GGCGCC
Tsp509I 132 AATT ^AATT
1434 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcyI AluI AspHI BanII
BbvI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
PstI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I TspRI