Restriction map for
pF1KE0060
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaHI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MfeI
MluI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
ApaI 149 GGGCCC GGGCC^C
AvrII 1063 CCTAGG C^CTAGG
BalI 1460 TGGCCA TGG^CCA
BciVI 1378 GGATAC GTATCC(6/5)
BglII 1122 AGATCT A^GATCT
BlnI 1063 CCTAGG C^CTAGG
BsaBI 1272 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 841 GGTCTC GGTCTC(1/5)
BsmBI 621 GAGACG CGTCTC(1/5)
BspLU11I 689 ACATGT A^CATGT
BspMI 1659 ACCTGC ACCTGC(4/8)
BsrI 535 CCAGT ACTGG(1/-1)
BstAPI 1186 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 396 GGTCACC G^GT[ACGT]ACC
BstPI 396 GGTCACC G^GT[ACGT]ACC
DraI 558 TTTAAA TTT^AAA
DraIII 466 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1460 TGGCCA [CT]^GGCC[GA]
EcoO65I 396 GGTCACC G^GT[ACGT]ACC
EcoRI 8 GAATTC G^AATTC
HaeII 213 GGCGCT [GA]GCGC^[CT]
KspI 1372 CCGCGG CCGC^GG
MaeII 668 ACGT A^CGT
MamI 1272 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1460 TGGCCA TGG^CCA
MscI 1460 TGGCCA TGG^CCA
NspI 689 ACATGT [GA]CATG^[CT]
PflMI 234 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 1372 CCGCGG CCGC^GG
SmaI 146 CCCGGG CCC^GGG
TspRI 1263 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 234 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 146 CCCGGG C^CCGGG
XmaI 146 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 89 TCCGGA T^CCGGA
438 TCCGGA
AcsI 8 GAATTC [GA]^AATT[CT]
562 AAATTT
AflIII 666 ACACGT A^C[GA][CT]GT
689 ACATGT
AlwI 739 GATCC GGATC(4/5)
1078 GATCC
AlwNI 78 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1042 CAGAGACTG
ApoI 8 GAATTC [GA]^AATT[CT]
562 AAATTT
AspI 391 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCCAGTC
AvaI 146 CCCGGG C^[CT]CG[GA]G
777 CCCGAG
BanII 149 GGGCCC G[GA]GC[CT]^C
983 GAGCCC
BmyI 149 GGGCCC G[AGT]GC[ACT]^C
983 GAGCCC
BseAI 89 TCCGGA T^CCGGA
438 TCCGGA
BsoBI 146 CCCGGG C^[CT]CG[GA]G
777 CCCGAG
Bsp1286I 149 GGGCCC G[AGT]GC[ACT]^C
983 GAGCCC
BspEI 89 TCCGGA T^CCGGA
438 TCCGGA
BsrBI 129 GAGCGG CCGCTC(-3/-3)
250 GAGCGG
BsrFI 237 GCCGGT [GA]^CCGG[CT]
1510 ACCGGC
BstYI 1077 AGATCC [GA]^GATC[CT]
1122 AGATCT
Cfr10I 237 GCCGGT [GA]^CCGG[CT]
1510 ACCGGC
DrdI 109 GACGCTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
370 GACCTCTATGTC
FauI 131 GCGGG CCCGC(4/6)
1374 GCGGG
HincII 642 GTCAAC GT[CT]^[GA]AC
1320 GTCAAC
HindII 642 GTCAAC GT[CT]^[GA]AC
1320 GTCAAC
MflI 1077 AGATCC [GA]^GATC[CT]
1122 AGATCT
MroI 89 TCCGGA T^CCGGA
438 TCCGGA
MseI 559 TTAA T^TAA
1012 TTAA
MspA1I 1372 CCGCGG C[AC]G^C[GT]G
1671 CAGCGG
NcoI 806 CCATGG C^CATGG
1457 CCATGG
PflFI 391 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCCAGTC
PpuMI 79 AGGTCCT [GA]G^G[AT]CC[CT]
368 AGGACCT
PstI 1048 CTGCAG CTGCA^G
1118 CTGCAG
SmlI 180 CTTGAG C^T[CT][GA]AG
571 CTCAAG
Tru9I 559 TTAA T^TAA
1012 TTAA
Tsp45I 397 GTCAC ^GT[GC]AC
946 GTGAC
Tth111I 391 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCCAGTC
XcmI 846 CCAAGTGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1457 CCATGGCCAACTTGG
XhoII 1077 AGATCC [GA]^GATC[CT]
1122 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbsI BbvI BcnI BfaI BpmI
BpuAI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI BsrDI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp509I TthHB8I