Restriction map for
pF1KE0058
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsiWI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRV FauI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MfeI MunI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 1530 CGCG CG^CG
AccIII 372 TCCGGA T^CCGGA
AcyI 42 GACGCC G[GA]^CG[CT]C
AflIII 1529 ACGCGT A^C[GA][CT]GT
ApaI 2106 GGGCCC GGGCC^C
AspHI 1297 GAGCAC G[AT]GC[AT]^C
AvaI 1580 CTCGGG C^[CT]CG[GA]G
BbsI 524 GTCTTC GAAGAC(2/6)
BbvCI 1858 GCTGAGG CCTCAGC(-5/-2)
BcgI 1607 GCAGATGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 562 GGATAC GTATCC(6/5)
BclI 2 TGATCA T^GATCA
BglII 1024 AGATCT A^GATCT
BpuAI 524 GTCTTC GAAGAC(2/6)
BsaBI 1235 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GACGCC G[GA]^CG[CT]C
BsaWI 372 TCCGGA [AT]^CCGG[AT]
BseAI 372 TCCGGA T^CCGGA
BsiEI 300 CGGTCG CG[GA][CT]^CG
BsiHKAI 1297 GAGCAC G[AT]GC[AT]^C
BsmBI 632 CGTCTC CGTCTC(1/5)
BsoBI 1580 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1043 TGTACA T^GTACA
BspEI 372 TCCGGA T^CCGGA
BspHI 1931 TCATGA T^CATGA
BsrDI 1461 CATTGC GCAATG(2/0)
BsrFI 1651 GCCGGT [GA]^CCGG[CT]
BsrGI 1043 TGTACA T^GTACA
BstEII 1413 GGTAACC G^GT[ACGT]ACC
BstPI 1413 GGTAACC G^GT[ACGT]ACC
BstUI 1530 CGCG CG^CG
Cfr10I 1651 GCCGGT [GA]^CCGG[CT]
DraIII 1363 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 891 CCATGG C^C[GA][CT]GG
EcoNI 1476 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1413 GGTAACC G^GT[ACGT]ACC
EcoT22I 1611 ATGCAT ATGCA^T
FbaI 2 TGATCA T^GATCA
Hin1I 42 GACGCC G[GA]^CG[CT]C
HindIII 956 AAGCTT A^AGCTT
MamI 1235 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1529 ACGCGT A^CGCGT
MroI 372 TCCGGA T^CCGGA
MslI 1758 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1624 CAGCTG C[AC]G^C[GT]G
MvnI 1530 CGCG CG^CG
NcoI 891 CCATGG C^CATGG
NheI 748 GCTAGC G^CTAGC
NsiI 1611 ATGCAT ATGCA^T
PflMI 1362 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 136 CTGCAG CTGCA^G
PvuII 1624 CAGCTG CAG^CTG
RcaI 1931 TCATGA T^CATGA
ScaI 1213 AGTACT AGT^ACT
SmlI 1778 CTCAAG C^T[CT][GA]AG
SphI 51 GCATGC GCATG^C
SspBI 1043 TGTACA T^GTACA
Tsp45I 1754 GTGAC ^GT[GC]AC
Van91I 1362 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1358 CCAACCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 344 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
605 CAGCACCTG
Asp700I 954 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1912 GAATCCCTTC
BanI 798 GGCACC G^G[CT][GA]CC
1973 GGCACC
BsaI 98 GGTCTC GGTCTC(1/5)
1468 GGTCTC
BsgI 611 CTGCAC GTGCAG(16/14)
1343 GTGCAG
BsmI 1049 GAATGC GAATGC(1/-1)
582 GCATTC
BspMI 609 ACCTGC ACCTGC(4/8)
821 ACCTGC
BstXI 885 CCAGGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAGTGGACTGG
BstYI 1024 AGATCT [GA]^GATC[CT]
1240 AGATCC
CfoI 141 GCGC GCG^C
2049 GCGC
EcoRI 339 GAATTC G^AATTC
833 GAATTC
HgaI 42 GACGC GACGC(5/10)
1528 GACGC
HhaI 141 GCGC GCG^C
2049 GCGC
HinP1I 141 GCGC G^CGC
2049 GCGC
MflI 1024 AGATCT [GA]^GATC[CT]
1240 AGATCC
NspI 51 GCATGC [GA]CATG^[CT]
1763 ACATGC
PpuMI 240 AGGTCCT [GA]G^G[AT]CC[CT]
1437 AGGACCT
SexAI 1409 ACCTGGT A^CC[AT]GGT
1475 ACCTGGT
SpeI 291 ACTAGT A^CTAGT
1662 ACTAGT
TaqI 1406 TCGA T^CGA
1472 TCGA
TthHB8I 1406 TCGA T^CGA
1472 TCGA
XhoII 1024 AGATCT [GA]^GATC[CT]
1240 AGATCC
XmnI 954 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1912 GAATCCCTTC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI