Restriction map for
pF1KE0052
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 2795 TCCGGA T^CCGGA
AclI 2485 AACGTT AA^CGTT
AflII 1921 CTTAAG C^TTAAG
AflIII 217 ACATGT A^C[GA][CT]GT
AspI 960 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 172 CCCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BanI 176 GGTGCC G^G[CT][GA]CC
BfrI 1921 CTTAAG C^TTAAG
BsaI 405 GGTCTC GGTCTC(1/5)
BsaWI 2795 TCCGGA [AT]^CCGG[AT]
BseAI 2795 TCCGGA T^CCGGA
BsoBI 172 CCCGGG C^[CT]CG[GA]G
BspEI 2795 TCCGGA T^CCGGA
BspLU11I 217 ACATGT A^CATGT
BspMI 20 ACCTGC ACCTGC(4/8)
BsrDI 2498 GCAATG GCAATG(2/0)
BstEII 1273 GGTGACC G^GT[ACGT]ACC
BstPI 1273 GGTGACC G^GT[ACGT]ACC
BstXI 53 CCAGGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 685 TTTAAA TTT^AAA
DraIII 1668 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1917 GACTCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO65I 1273 GGTGACC G^GT[ACGT]ACC
FauI 2710 GCGGG CCCGC(4/6)
HincII 1571 GTCAAC GT[CT]^[GA]AC
HindII 1571 GTCAAC GT[CT]^[GA]AC
HindIII 1843 AAGCTT A^AGCTT
MluNI 2 TGGCCA TGG^CCA
MroI 2795 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
NcoI 1732 CCATGG C^CATGG
NruI 3100 TCGCGA TCG^CGA
PflFI 960 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2485 AACGTT AA^CGTT
SapI 2907 GAAGAGC GCTCTTC(1/4)
SexAI 1887 ACCTGGT A^CC[AT]GGT
SmaI 172 CCCGGG CCC^GGG
SspI 2621 AATATT AAT^ATT
StuI 3005 AGGCCT AGG^CCT
Tth111I 960 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2412 TCTAGA T^CTAGA
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 2309 GTAGAC GT^[AC][GT]AC
2734 GTCTAC
AccII 184 CGCG CG^CG
3101 CGCG
AcyI 127 GACGCC G[GA]^CG[CT]C
2009 GACGCC
Asp700I 655 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2841 GAAACATTTC
BanII 136 GAGCTC G[GA]GC[CT]^C
2910 GAGCTC
BcnI 172 CCCGG CC^[GC]GG
173 CCGGG
BglII 1905 AGATCT A^GATCT
2557 AGATCT
BsaBI 1336 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1363 GATCGGGATC
BsaHI 127 GACGCC G[GA]^CG[CT]C
2009 GACGCC
BsmFI 36 GGGAC GGGAC(10/14)
2898 GTCCC
BstUI 184 CGCG CG^CG
3101 CGCG
CfoI 24 GCGC GCG^C
2876 GCGC
DraII 36 GGGACCT [GA]G^G[ACGT]CC[CT]
2928 AGGACCC
EcoO109I 36 GGGACCT [GA]G^G[ACGT]CC[CT]
2928 AGGACCC
EcoRI 655 GAATTC G^AATTC
1185 GAATTC
HapII 173 CCGG C^CGG
2796 CCGG
HgaI 127 GACGC GACGC(5/10)
2009 GACGC
HhaI 24 GCGC GCG^C
2876 GCGC
Hin1I 127 GACGCC G[GA]^CG[CT]C
2009 GACGCC
HinP1I 24 GCGC G^CGC
2876 GCGC
HpaII 173 CCGG C^CGG
2796 CCGG
MamI 1336 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1363 GATCGGGATC
MspI 173 CCGG C^CGG
2796 CCGG
MvnI 184 CGCG CG^CG
3101 CGCG
NciI 172 CCCGG CC^[GC]GG
173 CCGGG
PpuMI 36 GGGACCT [GA]G^G[AT]CC[CT]
2928 AGGACCC
PstI 141 CTGCAG CTGCA^G
2165 CTGCAG
SacI 136 GAGCTC GAGCT^C
2910 GAGCTC
SmlI 1921 CTTAAG C^T[CT][GA]AG
2004 CTCAAG
XmnI 655 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2841 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII