Restriction map for
pF1KB7276
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MboI
MfeI MflI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 70 GCGG CCGC(-3/-1)
AlwNI 346 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 413 CTCGAG C^[CT]CG[GA]G
BalI 326 TGGCCA TGG^CCA
BbrPI 886 CACGTG CAC^GTG
BsaAI 886 CACGTG [CT]AC^GT[GA]
BseRI 766 GAGGAG GAGGAG(10/8)
BsgI 1034 GTGCAG GTGCAG(16/14)
BsiYI 683 CCTTGTTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 683 CCTTGTTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 468 GAATGC GAATGC(1/-1)
BsoBI 413 CTCGAG C^[CT]CG[GA]G
BstAPI 868 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 326 TGGCCA [CT]^GGCC[GA]
EarI 763 GAAGAG CTCTTC(1/4)
EcoNI 683 CCTTGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1138 GAATTC G^AATTC
EcoT22I 185 ATGCAT ATGCA^T
HaeIII 327 GGCC GG^CC
HincII 1085 GTCAAC GT[CT]^[GA]AC
HindII 1085 GTCAAC GT[CT]^[GA]AC
HphI 680 TCACC GGTGA(8/7)
Ksp632I 763 GAAGAG CTCTTC(1/4)
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
NlaIV 311 GGTTCC GG[ACGT]^[ACGT]CC
NsiI 185 ATGCAT ATGCA^T
NspI 66 GCATGC [GA]CATG^[CT]
PaeR7I 413 CTCGAG C^TCGAG
PmaCI 886 CACGTG CAC^GTG
PmlI 886 CACGTG CAC^GTG
SmlI 413 CTCGAG C^T[CT][GA]AG
SphI 66 GCATGC GCATG^C
TaqI 414 TCGA T^CGA
TthHB8I 414 TCGA T^CGA
XbaI 1023 TCTAGA T^CTAGA
XcmI 832 CCACTTGTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 413 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 838 GTAC GT^AC
1110 GTAC
AluI 216 AGCT AG^CT
1041 AGCT
AvaII 654 GGACC G^G[AT]CC
829 GGACC
BbvI 1036 GCAGC GCAGC(8/12)
1039 GCAGC
BsmFI 320 GGGAC GGGAC(10/14)
348 GTCCC
BsrDI 638 GCAATG GCAATG(2/0)
952 GCAATG
BssKI 28 CCTGG ^CC[ACGT]GG
1069 CCAGG
BstNI 28 CCTGG CC^[AT]GG
1069 CCAGG
Cac8I 66 GCATGC GC[ACGT]^[ACGT]GC
932 GCTTGC
Cfr13I 654 GGACC G^G[ACGT]CC
829 GGACC
EcoRII 28 CCTGG ^CC[AT]GG
1069 CCAGG
EcoT14I 377 CCAAGG C^C[AT][AT]GG
628 CCAAGG
MaeII 139 ACGT A^CGT
887 ACGT
MnlI 652 GAGG CCTC(7/6)
766 GAGG
MvaI 28 CCTGG CC^[AT]GG
1069 CCAGG
MwoI 868 GCATTTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
957 GCTACTTCAGC
PleI 913 GAGTC GAGTC(4/5)
1083 GAGTC
RsaI 838 GTAC GT^AC
1110 GTAC
Sau96I 654 GGACC G^G[ACGT]CC
829 GGACC
ScrFI 28 CCTGG CC^[ACGT]GG
1069 CCAGG
SspI 115 AATATT AAT^ATT
358 AATATT
StyI 377 CCAAGG C^C[AT][AT]GG
628 CCAAGG
TseI 1036 GCAGC G^C[AT]GC
1039 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI ApoI BfaI BsaJI BsrI
BstF5I DdeI Fnu4HI FokI HinfI
ItaI MaeI MaeIII MboII MseI
NlaIII SfaNI TfiI Tru9I Tsp45I
Tsp509I TspRI