Restriction map for
pF1KB6636
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BcgI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MaeII MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Alw44I 1271 GTGCAC G^TGCAC
AlwNI 107 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1271 GTGCAC G^TGCAC
AspHI 1271 GTGCAC G[AT]GC[AT]^C
AvaII 153 GGACC G^G[AT]CC
BamHI 369 GGATCC G^GATCC
BbvCI 627 CCTCAGC CCTCAGC(-5/-2)
BciVI 910 GTATCC GTATCC(6/5)
BfaI 784 CTAG C^TAG
BlpI 757 GCTCAGC GC^T[ACGT]AGC
BmyI 1271 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 757 GCTCAGC GC^T[ACGT]AGC
BsaJI 88 CCTTGG C^C[ACGT][ACGT]GG
BseRI 742 GAGGAG GAGGAG(10/8)
BsiHKAI 1271 GTGCAC G[AT]GC[AT]^C
BsmAI 37 GAGAC GTCTC(1/5)
Bsp1286I 1271 GTGCAC G[AGT]GC[ACT]^C
BsrDI 937 GCAATG GCAATG(2/0)
BssKI 373 CCTGG ^CC[ACGT]GG
BstNI 373 CCTGG CC^[AT]GG
BstYI 369 GGATCC [GA]^GATC[CT]
Cac8I 996 GCATGC GC[ACGT]^[ACGT]GC
CelII 757 GCTCAGC GC^T[ACGT]AGC
CfoI 994 GCGC GCG^C
Cfr13I 153 GGACC G^G[ACGT]CC
DraI 954 TTTAAA TTT^AAA
EcoRII 373 CCTGG ^CC[AT]GG
EcoRV 295 GATATC GAT^ATC
EcoT14I 88 CCTTGG C^C[AT][AT]GG
HaeIII 540 GGCC GG^CC
HhaI 994 GCGC GCG^C
HinP1I 994 GCGC G^CGC
HincII 1312 GTTGAC GT[CT]^[GA]AC
HindII 1312 GTTGAC GT[CT]^[GA]AC
MaeI 784 CTAG C^TAG
MflI 369 GGATCC [GA]^GATC[CT]
MspA1I 914 CCGCTG C[AC]G^C[GT]G
MvaI 373 CCTGG CC^[AT]GG
SapI 1126 GAAGAGC GCTCTTC(1/4)
Sau96I 153 GGACC G^G[ACGT]CC
ScrFI 373 CCTGG CC^[ACGT]GG
SmlI 842 CTCAAG C^T[CT][GA]AG
SphI 996 GCATGC GCATG^C
SspI 858 AATATT AAT^ATT
StyI 88 CCTTGG C^C[AT][AT]GG
TaqI 1217 TCGA T^CGA
TspRI 1163 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1217 TCGA T^CGA
XhoII 369 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 148 GTAC GT^AC
705 GTAC
AlwI 370 GATCC GGATC(4/5)
369 GGATC
HindIII 824 AAGCTT A^AGCTT
922 AAGCTT
MaeIII 158 GTGAC ^GT[ACGT]AC
617 GTCAC
MfeI 344 CAATTG C^AATTG
716 CAATTG
MunI 344 CAATTG C^AATTG
716 CAATTG
PleI 116 GAGTC GAGTC(4/5)
243 GAGTC
PstI 104 CTGCAG CTGCA^G
661 CTGCAG
RsaI 148 GTAC GT^AC
705 GTAC
SfcI 104 CTGCAG C^T[GA][CT]AG
661 CTGCAG
Tsp45I 158 GTGAC ^GT[GC]AC
617 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AcsI AluI ApoI BbsI
BbvI BpmI BpuAI BsrI BstF5I
DdeI DpnII EarI Fnu4HI FokI
HinfI HphI ItaI Ksp632I MboI
MboII MnlI MseI MslI MwoI
NdeII NlaIII NlaIV NspI Sau3AI
SfaNI TfiI Tru9I TseI Tsp509I