Restriction map for
pF1KE0045
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccII AccIII AclI AcyI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AspEI AspI AvaI
AvaII AviII AvrII BalI BanII
BbeI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KspI MfeI
MluI MluNI MroI MscI MspI
MunI MvaI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI Sau96I SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 779 GGTACC G^GTACC
AccI 1618 GTAGAC GT^[AC][GT]AC
AciI 1097 GCGG CCGC(-3/-1)
AflII 1974 CTTAAG C^TTAAG
Alw44I 1569 GTGCAC G^TGCAC
ApaLI 1569 GTGCAC G^TGCAC
AseI 651 ATTAAT AT^TAAT
AsnI 651 ATTAAT AT^TAAT
Asp718I 779 GGTACC G^GTACC
BamHI 1252 GGATCC G^GATCC
BanI 779 GGTACC G^G[CT][GA]CC
BbrPI 222 CACGTG CAC^GTG
BbvI 1036 GCAGC GCAGC(8/12)
BclI 1662 TGATCA T^GATCA
BfrI 1974 CTTAAG C^TTAAG
BsaAI 222 CACGTG [CT]AC^GT[GA]
BsaBI 1855 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 371 CCACTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 371 CCACTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1898 GAATGC GAATGC(1/-1)
Bsp1407I 226 TGTACA T^GTACA
BspHI 723 TCATGA T^CATGA
BspLU11I 454 ACATGT A^CATGT
BsrGI 226 TGTACA T^GTACA
BstAPI 1237 GCAAGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1237 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 353 GCGC GCG^C
DraI 124 TTTAAA TTT^AAA
FbaI 1662 TGATCA T^GATCA
HaeIII 1692 GGCC GG^CC
HhaI 353 GCGC GCG^C
HinP1I 353 GCGC G^CGC
HindIII 129 AAGCTT A^AGCTT
KpnI 779 GGTACC GGTAC^C
MamI 1855 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 1237 GCAAGCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1082 CATATG CA^TATG
PleI 1598 GAGTC GAGTC(4/5)
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PshBI 651 ATTAAT AT^TAAT
RcaI 723 TCATGA T^CATGA
SfcI 435 CTGTAG C^T[GA][CT]AG
SmlI 1974 CTTAAG C^T[CT][GA]AG
SspBI 226 TGTACA T^GTACA
StuI 1691 AGGCCT AGG^CCT
TaqI 1117 TCGA T^CGA
TseI 1036 GCAGC G^C[AT]GC
TthHB8I 1117 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflIII 223 ACGTGT A^C[GA][CT]GT
454 ACATGT
AspHI 636 GTGCTC G[AT]GC[AT]^C
1569 GTGCAC
BmyI 636 GTGCTC G[AGT]GC[ACT]^C
1569 GTGCAC
BsaI 242 GGTCTC GGTCTC(1/5)
605 GGTCTC
BsgI 1034 GTGCAG GTGCAG(16/14)
1507 GTGCAG
BsiHKAI 636 GTGCTC G[AT]GC[AT]^C
1569 GTGCAC
Bsp1286I 636 GTGCTC G[AGT]GC[ACT]^C
1569 GTGCAC
BsrDI 1274 GCAATG GCAATG(2/0)
1902 GCAATG
DsaI 924 CCATGG C^C[GA][CT]GG
1110 CCATGG
EcoT22I 978 ATGCAT ATGCA^T
1085 ATGCAT
Fnu4HI 1036 GCAGC GC^[ACGT]GC
1097 GCGGC
HphI 1825 GGTGA GGTGA(8/7)
1665 TCACC
ItaI 1036 GCAGC GC^[ACGT]GC
1097 GCGGC
MaeII 223 ACGT A^CGT
942 ACGT
MspA1I 557 CAGCTG C[AC]G^C[GT]G
1865 CAGCTG
NcoI 924 CCATGG C^CATGG
1110 CCATGG
NsiI 978 ATGCAT ATGCA^T
1085 ATGCAT
PvuII 557 CAGCTG CAG^CTG
1865 CAGCTG
SfaNI 1271 GATGC GCATC(5/9)
1191 GCATC
SspI 110 AATATT AAT^ATT
1280 AATATT
TfiI 787 GAATC G^A[AT]TC
1555 GAATC
Tsp45I 1078 GTGAC ^GT[GC]AC
1907 GTGAC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
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AcsI AfaI AluI AlwI ApoI
Asp700I BbsI BfaI BpmI BpuAI
BsaJI BseRI BsmAI BsrI BstF5I
BstYI DdeI DpnII EarI EcoT14I
FokI HinfI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI NdeII NlaIII NlaIV NspI
RsaI SapI Sau3AI StyI Tru9I
Tsp509I TspRI XhoII XmnI