##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          300  GTAC                 GT^AC
AlwI          339  GATCC                GGATC(4/5)
AviII         353  TGCGCA               TGC^GCA
BanI           18  GGCACC               G^G[CT][GA]CC
BfaI           29  CTAG                 C^TAG
BpmI          208  CTGGAG               CTGGAG(16/14)
BseRI          81  CTCCTC               GAGGAG(10/8)
BsgI          251  GTGCAG               GTGCAG(16/14)
BsmFI         266  GGGAC                GGGAC(10/14)
BsrBI          92  CCGCTC               CCGCTC(-3/-3)
BstYI         338  AGATCC               [GA]^GATC[CT]
DpnII         339  GATC                 ^GATC
EarI          198  GAAGAG               CTCTTC(1/4)
FspI          353  TGCGCA               TGC^GCA
HaeII         202  AGCGCC               [GA]GCGC^[CT]
HgaI          401  GCGTC                GACGC(5/10)
HinfI         322  GACTC                G^A[ACGT]TC
Ksp632I       198  GAAGAG               CTCTTC(1/4)
MaeI           29  CTAG                 C^TAG
MboI          339  GATC                 ^GATC
MflI          338  AGATCC               [GA]^GATC[CT]
NdeII         339  GATC                 ^GATC
NlaIII        143  CATG                 CATG^
NspI          142  GCATGT               [GA]CATG^[CT]
PleI          322  GACTC                GAGTC(4/5)
RsaI          300  GTAC                 GT^AC
SacI           61  GAGCTC               GAGCT^C
SapI          198  GAAGAGC              GCTCTTC(1/4)
Sau3AI        339  GATC                 ^GATC
XhoII         338  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

ApaI           43  GGGCCC               GGGCC^C
              233  GGGCCC              
AspHI          61  GAGCTC               G[AT]GC[AT]^C
              379  GTGCTC              
BlpI           94  GCTCAGC              GC^T[ACGT]AGC
              381  GCTCAGC             
Bpu1102I       94  GCTCAGC              GC^T[ACGT]AGC
              381  GCTCAGC             
BsiHKAI        61  GAGCTC               G[AT]GC[AT]^C
              379  GTGCTC              
CelII          94  GCTCAGC              GC^T[ACGT]AGC
              381  GCTCAGC             
HapII         231  CCGG                 C^CGG
              238  CCGG                
HpaII         231  CCGG                 C^CGG
              238  CCGG                
MspI          231  CCGG                 C^CGG
              238  CCGG                
PvuII          13  CAGCTG               CAG^CTG
               97  CAGCTG              
SmaI          230  CCCGGG               CCC^GGG
              237  CCCGGG              
XmaCI         230  CCCGGG               C^CCGGG
              237  CCCGGG              
XmaI          230  CCCGGG               C^CCGGG
              237  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      FauI        
Fnu4HI      HaeIII      HhaI        HinP1I      ItaI        
MboII       MnlI        MspA1I      MvaI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TseI