Restriction map for
pF1KB0934
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcnI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspLU11I BspMI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 987 CCGC CCGC(-3/-1)
AflII 667 CTTAAG C^TTAAG
AlwNI 875 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1072 ATTAAT AT^TAAT
AsnI 1072 ATTAAT AT^TAAT
AvaII 1092 GGTCC G^G[AT]CC
BamHI 397 GGATCC G^GATCC
BcgI 272 GCACTTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 343 GGATAC GTATCC(6/5)
BclI 605 TGATCA T^GATCA
BfaI 638 CTAG C^TAG
BfrI 667 CTTAAG C^TTAAG
BmyI 956 GTGCCC G[AGT]GC[ACT]^C
BsaI 936 GAGACC GGTCTC(1/5)
BsaJI 805 CCTTGG C^C[ACGT][ACGT]GG
Bsp1286I 956 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 681 TGTACA T^GTACA
BspHI 602 TCATGA T^CATGA
BsrBI 987 CCGCTC CCGCTC(-3/-3)
BsrDI 787 CATTGC GCAATG(2/0)
BsrGI 681 TGTACA T^GTACA
BssKI 621 CCAGG ^CC[ACGT]GG
BstNI 621 CCAGG CC^[AT]GG
BstYI 397 GGATCC [GA]^GATC[CT]
Cac8I 842 GCAAGC GC[ACGT]^[ACGT]GC
EcoNI 859 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 481 GAATTC G^AATTC
EcoRII 621 CCAGG ^CC[AT]GG
EcoT14I 805 CCTTGG C^C[AT][AT]GG
EcoT22I 128 ATGCAT ATGCA^T
FauI 986 CCCGC CCCGC(4/6)
FbaI 605 TGATCA T^GATCA
HaeIII 502 GGCC GG^CC
MaeI 638 CTAG C^TAG
MaeIII 913 GTGAC ^GT[ACGT]AC
MflI 397 GGATCC [GA]^GATC[CT]
MspA1I 179 CAGCTG C[AC]G^C[GT]G
MvaI 621 CCAGG CC^[AT]GG
MwoI 331 GCACAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 488 CATATG CA^TATG
NsiI 128 ATGCAT ATGCA^T
PleI 877 GACTC GAGTC(4/5)
PshAI 889 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1072 ATTAAT AT^TAAT
PvuII 179 CAGCTG CAG^CTG
RcaI 602 TCATGA T^CATGA
ScrFI 621 CCAGG CC^[ACGT]GG
SfcI 92 CTGTAG C^T[GA][CT]AG
SspBI 681 TGTACA T^GTACA
StyI 805 CCTTGG C^C[AT][AT]GG
Tsp45I 913 GTGAC ^GT[GC]AC
XhoII 397 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 43 GTCTAC GT^[AC][GT]AC
799 GTAGAC
AfaI 415 GTAC GT^AC
682 GTAC
AlwI 398 GATCC GGATC(4/5)
397 GGATC
BsiYI 178 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCTTCTCAAGG
BslI 178 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCTTCTCAAGG
BsmI 222 GAATGC GAATGC(1/-1)
73 GCATTC
BstF5I 17 GGATG GGATG(2/0)
732 GGATG
BstXI 722 CCATTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
960 CCAGTCATTTGG
Cfr13I 501 GGGCC G^G[ACGT]CC
1092 GGTCC
DraI 189 TTTAAA TTT^AAA
324 TTTAAA
FokI 17 GGATG GGATG(9/13)
732 GGATG
HphI 451 GGTGA GGTGA(8/7)
1111 GGTGA
NlaIV 397 GGATCC GG[ACGT]^[ACGT]CC
500 GGGGCC
RsaI 415 GTAC GT^AC
682 GTAC
Sau96I 501 GGGCC G^G[ACGT]CC
1092 GGTCC
SmlI 667 CTTAAG C^T[CT][GA]AG
863 CTCAAG
TspRI 836 CAGTG CA[GC]TG[ACGT][ACGT]^
954 CAGTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BpuAI BsmAI BsrI DdeI DpnII
Fnu4HI HinfI ItaI MboI MboII
MnlI MseI MslI NdeII NlaIII
Sau3AI SfaNI TfiI Tru9I TseI
Tsp509I