Restriction map for
pF1KB8178
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspI AvaI
AvaII AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
BssSI BstBI BstUI CfoI Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 943 GGTACC G^GTACC
AccI 1104 GTATAC GT^[AC][GT]AC
AciI 882 CCGC CCGC(-3/-1)
AclI 734 AACGTT AA^CGTT
AocI 756 CCTAAGG CC^T[ACGT]AGG
Asp700I 289 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 943 GGTACC G^GTACC
BamHI 787 GGATCC G^GATCC
BanI 943 GGTACC G^G[CT][GA]CC
BanII 1267 GAGCTC G[GA]GC[CT]^C
BciVI 1287 GGATAC GTATCC(6/5)
BcnI 939 CCGGG CC^[GC]GG
BfaI 1297 CTAG C^TAG
BglI 565 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 395 AGATCT A^GATCT
BlpI 888 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 888 GCTCAGC GC^T[ACGT]AGC
BseRI 580 GAGGAG GAGGAG(10/8)
BsgI 367 GTGCAG GTGCAG(16/14)
BsmFI 805 GTCCC GGGAC(10/14)
Bsp1407I 239 TGTACA T^GTACA
BsrDI 327 CATTGC GCAATG(2/0)
BsrGI 239 TGTACA T^GTACA
Bst1107I 1104 GTATAC GTA^TAC
BstAPI 118 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 487 GGTGACC G^GT[ACGT]ACC
BstPI 487 GGTGACC G^GT[ACGT]ACC
BstZ17I 1104 GTATAC GTA^TAC
Bsu36I 756 CCTAAGG CC^T[ACGT]AGG
CelII 888 GCTCAGC GC^T[ACGT]AGC
DrdI 363 GACTGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 756 CCTAAGG CC^T[ACGT]AGG
EcoO65I 487 GGTGACC G^GT[ACGT]ACC
EcoT14I 834 CCTTGG C^C[AT][AT]GG
HapII 939 CCGG C^CGG
HpaII 939 CCGG C^CGG
KpnI 943 GGTACC GGTAC^C
MaeI 1297 CTAG C^TAG
MaeII 735 ACGT A^CGT
MspI 939 CCGG C^CGG
NciI 939 CCGGG CC^[GC]GG
PleI 1261 GAGTC GAGTC(4/5)
Psp1406I 734 AACGTT AA^CGTT
PstI 539 CTGCAG CTGCA^G
SacI 1267 GAGCTC GAGCT^C
SfcI 539 CTGCAG C^T[GA][CT]AG
SspBI 239 TGTACA T^GTACA
StyI 834 CCTTGG C^C[AT][AT]GG
XcmI 566 CCACCCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 289 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 777 ACATGT A^C[GA][CT]GT
1100 ACATGT
AhdI 431 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1189 GACAGCTGGTC
AspEI 431 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1189 GACAGCTGGTC
AspHI 583 GAGCAC G[AT]GC[AT]^C
1267 GAGCTC
BmyI 583 GAGCAC G[AGT]GC[ACT]^C
1267 GAGCTC
BsiHKAI 583 GAGCAC G[AT]GC[AT]^C
1267 GAGCTC
Bsp1286I 583 GAGCAC G[AGT]GC[ACT]^C
1267 GAGCTC
BspLU11I 777 ACATGT A^CATGT
1100 ACATGT
BstXI 720 CCATTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1143 CCAAAGGTTTGG
BstYI 395 AGATCT [GA]^GATC[CT]
787 GGATCC
Eam1105I 431 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1189 GACAGCTGGTC
EcoT22I 177 ATGCAT ATGCA^T
1157 ATGCAT
MboII 27 TCTTC GAAGA(8/7)
303 TCTTC
MfeI 86 CAATTG C^AATTG
1087 CAATTG
MflI 395 AGATCT [GA]^GATC[CT]
787 GGATCC
MslI 721 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
772 CATACACATG
MspA1I 37 CAGCTG C[AC]G^C[GT]G
1191 CAGCTG
MunI 86 CAATTG C^AATTG
1087 CAATTG
NsiI 177 ATGCAT ATGCA^T
1157 ATGCAT
PflMI 570 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAATTGTTGG
PvuII 37 CAGCTG CAG^CTG
1191 CAGCTG
SfaNI 1139 GATGC GCATC(5/9)
846 GCATC
TaqI 297 TCGA T^CGA
1077 TCGA
TthHB8I 297 TCGA T^CGA
1077 TCGA
Van91I 570 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAATTGTTGG
XhoII 395 AGATCT [GA]^GATC[CT]
787 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BpmI BsaJI BsiYI
BslI BsmAI BsmI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI