Restriction map for
pF1KB8144
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI TaqI TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AluI 384 AGCT AG^CT
Asp700I 628 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 39 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 135 CTCGGG C^[CT]CG[GA]G
AvaII 525 GGACC G^G[AT]CC
BbsI 350 GAAGAC GAAGAC(2/6)
BcgI 537 CGAGAAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 105 CCGGG CC^[GC]GG
BfaI 578 CTAG C^TAG
BglI 104 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 630 AGATCT A^GATCT
BpmI 146 CTGGAG CTGGAG(16/14)
BpuAI 350 GAAGAC GAAGAC(2/6)
BsaI 587 GAGACC GGTCTC(1/5)
BsgI 545 GTGCAG GTGCAG(16/14)
BsmFI 151 GGGAC GGGAC(10/14)
BsoBI 135 CTCGGG C^[CT]CG[GA]G
BspMI 22 ACCTGC ACCTGC(4/8)
BsrDI 94 CATTGC GCAATG(2/0)
BstAPI 26 GCAGCGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 522 CCAGG CC^[AT]GG
DraI 361 TTTAAA TTT^AAA
DrdI 39 GACCACGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 102 TGGCCG [CT]^GGCC[GA]
EarI 540 GAAGAG CTCTTC(1/4)
EcoRII 522 CCAGG ^CC[AT]GG
EcoT14I 637 CCAAGG C^C[AT][AT]GG
Ksp632I 540 GAAGAG CTCTTC(1/4)
MaeI 578 CTAG C^TAG
MaeIII 693 GTCAC ^GT[ACGT]AC
MslI 551 CACTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 522 CCAGG CC^[AT]GG
NciI 105 CCGGG CC^[GC]GG
NdeI 744 CATATG CA^TATG
PflFI 39 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
StyI 637 CCAAGG C^C[AT][AT]GG
SwaI 360 ATTTAAAT ATTT^AAAT
Tsp45I 693 GTCAC ^GT[GC]AC
Tth111I 39 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 90 CCATCATTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 628 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 114 CGCG CG^CG
183 CGCG
AcsI 260 AAATTC [GA]^AATT[CT]
658 GAATTT
AlwI 81 GATCC GGATC(4/5)
598 GATCC
ApoI 260 AAATTC [GA]^AATT[CT]
658 GAATTT
BsaJI 105 CCGGGG C^C[ACGT][ACGT]GG
637 CCAAGG
BsiYI 50 CCTCCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
730 CCAGAGAAGGG
BslI 50 CCTCCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
730 CCAGAGAAGGG
BsmAI 587 GAGAC GTCTC(1/5)
328 GTCTC
BssKI 105 CCGGG ^CC[ACGT]GG
522 CCAGG
BstUI 114 CGCG CG^CG
183 CGCG
BstYI 80 AGATCC [GA]^GATC[CT]
630 AGATCT
HapII 105 CCGG C^CGG
310 CCGG
HpaII 105 CCGG C^CGG
310 CCGG
HphI 454 GGTGA GGTGA(8/7)
703 TCACC
MaeII 43 ACGT A^CGT
556 ACGT
MflI 80 AGATCC [GA]^GATC[CT]
630 AGATCT
MseI 362 TTAA T^TAA
782 TTAA
MspI 105 CCGG C^CGG
310 CCGG
MvnI 114 CGCG CG^CG
183 CGCG
PleI 750 GACTC GAGTC(4/5)
391 GAGTC
PstI 24 CTGCAG CTGCA^G
413 CTGCAG
ScrFI 105 CCGGG CC^[ACGT]GG
522 CCAGG
SfcI 24 CTGCAG C^T[GA][CT]AG
413 CTGCAG
TfiI 418 GAATC G^A[AT]TC
787 GAATC
Tru9I 362 TTAA T^TAA
782 TTAA
XhoII 80 AGATCC [GA]^GATC[CT]
630 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BbvI BsrI Cac8I CfoI
Cfr13I DdeI DpnII FauI Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MboI MboII MnlI MspA1I MwoI
NdeII NlaIII Sau3AI Sau96I TseI
Tsp509I TspRI