Restriction map for
pF1KB0432
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaI
BseAI BsiWI BspDI BspEI BspHI
BsrBI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 2448 GGTACC G^GTACC
AcsI 1357 AAATTC [GA]^AATT[CT]
AflIII 1919 ACATGT A^C[GA][CT]GT
AgeI 1033 ACCGGT A^CCGGT
Alw44I 1558 GTGCAC G^TGCAC
AocI 1654 CCTGAGG CC^T[ACGT]AGG
ApaLI 1558 GTGCAC G^TGCAC
ApoI 1357 AAATTC [GA]^AATT[CT]
Asp718I 2448 GGTACC G^GTACC
BbsI 897 GAAGAC GAAGAC(2/6)
BfaI 736 CTAG C^TAG
BglII 338 AGATCT A^GATCT
BlpI 1500 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1500 GCTGAGC GC^T[ACGT]AGC
BpuAI 897 GAAGAC GAAGAC(2/6)
BsaWI 1033 ACCGGT [AT]^CCGG[AT]
BsiEI 1996 CGGCCG CG[GA][CT]^CG
BsmBI 2194 CGTCTC CGTCTC(1/5)
BspLU11I 1919 ACATGT A^CATGT
BsrDI 1056 CATTGC GCAATG(2/0)
BssHII 2106 GCGCGC G^CGCGC
BssSI 1930 CACGAG CACGAG(-5/-1)
Bsu36I 1654 CCTGAGG CC^T[ACGT]AGG
CelII 1500 GCTGAGC GC^T[ACGT]AGC
DraIII 1114 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1606 GACAATGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1761 CCACGG C^C[GA][CT]GG
EagI 1996 CGGCCG C^GGCCG
EclXI 1996 CGGCCG C^GGCCG
Eco52I 1996 CGGCCG C^GGCCG
Eco81I 1654 CCTGAGG CC^T[ACGT]AGG
EcoNI 384 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 502 ATGCAT ATGCA^T
HaeII 850 AGCGCC [GA]GCGC^[CT]
HincII 1636 GTCAAC GT[CT]^[GA]AC
HindII 1636 GTCAAC GT[CT]^[GA]AC
KpnI 2448 GGTACC GGTAC^C
MaeI 736 CTAG C^TAG
MaeII 1447 ACGT A^CGT
MseI 1094 TTAA T^TAA
NsiI 502 ATGCAT ATGCA^T
PinAI 1033 ACCGGT A^CCGGT
PpuMI 2216 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1271 CAGCTG CAG^CTG
ScaI 1565 AGTACT AGT^ACT
SgrAI 1032 CACCGGTG C[GA]^CCGG[CT]G
SphI 1833 GCATGC GCATG^C
TfiI 809 GATTC G^A[AT]TC
Tru9I 1094 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1615 GTCTAC GT^[AC][GT]AC
2542 GTCTAC
AcyI 623 GGCGTC G[GA]^CG[CT]C
1745 GGCGTC
AvaI 1848 CTCGGG C^[CT]CG[GA]G
2353 CCCGAG
BbvCI 580 CCTCAGC CCTCAGC(-5/-2)
2461 GCTGAGG
BsaHI 623 GGCGTC G[GA]^CG[CT]C
1745 GGCGTC
BsmI 234 GAATGC GAATGC(1/-1)
504 GCATTC
BsoBI 1848 CTCGGG C^[CT]CG[GA]G
2353 CCCGAG
BsrFI 1033 ACCGGT [GA]^CCGG[CT]
1994 ACCGGC
BstAPI 504 GCATTCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1668 GCACCACCTGC
BstXI 1304 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2405 CCATCGTTGTGG
BstYI 338 AGATCT [GA]^GATC[CT]
701 AGATCC
Cfr10I 1033 ACCGGT [GA]^CCGG[CT]
1994 ACCGGC
EarI 1282 GAAGAG CTCTTC(1/4)
1911 GAAGAG
EcoT14I 78 CCAAGG C^C[AT][AT]GG
2150 CCAAGG
Hin1I 623 GGCGTC G[GA]^CG[CT]C
1745 GGCGTC
Ksp632I 1282 GAAGAG CTCTTC(1/4)
1911 GAAGAG
MaeIII 980 GTGAC ^GT[ACGT]AC
1165 GTGAC
MflI 338 AGATCT [GA]^GATC[CT]
701 AGATCC
SmlI 964 CTCAAG C^T[CT][GA]AG
1378 CTCAAG
StuI 1259 AGGCCT AGG^CCT
2552 AGGCCT
StyI 78 CCAAGG C^C[AT][AT]GG
2150 CCAAGG
Tsp45I 980 GTGAC ^GT[GC]AC
1165 GTGAC
XcmI 251 CCACAGTCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2477 CCAACCTGGCTCTGG
XhoII 338 AGATCT [GA]^GATC[CT]
701 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TseI Tsp509I TspRI
TthHB8I Van91I XmnI