Restriction map for
pF1KE0033
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 71 CGCG CG^CG
AflIII 1671 ACATGT A^C[GA][CT]GT
Aor51HI 52 AGCGCT AGC^GCT
ApaI 1231 GGGCCC GGGCC^C
AseI 1255 ATTAAT AT^TAAT
AsnI 1255 ATTAAT AT^TAAT
AvaI 1622 CCCGAG C^[CT]CG[GA]G
BbvI 55 GCTGC GCAGC(8/12)
BcnI 63 CCCGG CC^[GC]GG
BglI 58 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 641 AGATCT A^GATCT
BpmI 1109 CTCCAG CTGGAG(16/14)
BseRI 1467 CTCCTC GAGGAG(10/8)
BsgI 1501 GTGCAG GTGCAG(16/14)
BsoBI 1622 CCCGAG C^[CT]CG[GA]G
BspHI 723 TCATGA T^CATGA
BspLU11I 1671 ACATGT A^CATGT
BspMI 1641 ACCTGC ACCTGC(4/8)
BsrDI 1432 GCAATG GCAATG(2/0)
BstUI 71 CGCG CG^CG
BstXI 68 CCACGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 216 TTTAAA TTT^AAA
DrdI 1353 GACTAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 65 CGGCCA [CT]^GGCC[GA]
Eco47III 52 AGCGCT AGC^GCT
EcoRI 410 GAATTC G^AATTC
EcoRV 1122 GATATC GAT^ATC
HaeII 52 AGCGCT [GA]GCGC^[CT]
HapII 64 CCGG C^CGG
HgaI 915 GACGC GACGC(5/10)
HpaII 64 CCGG C^CGG
MaeII 788 ACGT A^CGT
MslI 69 CACGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 46 CAGCTG C[AC]G^C[GT]G
MspI 64 CCGG C^CGG
MvnI 71 CGCG CG^CG
NciI 63 CCCGG CC^[GC]GG
NspI 1671 ACATGT [GA]CATG^[CT]
PshBI 1255 ATTAAT AT^TAAT
PstI 93 CTGCAG CTGCA^G
PvuII 46 CAGCTG CAG^CTG
RcaI 723 TCATGA T^CATGA
TseI 55 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 513 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1045 GAAATTGTTC
AvaII 708 GGACC G^G[AT]CC
1414 GGACC
BlpI 48 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
BsaJI 415 CCAGGG C^C[ACGT][ACGT]GG
1623 CCGAGG
BsmI 1011 GAATGC GAATGC(1/-1)
634 GCATTC
BstAPI 1267 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1503 GCAGTTTGTGC
BstNI 390 CCTGG CC^[AT]GG
415 CCAGG
Cac8I 25 GCGGGC GC[ACGT]^[ACGT]GC
630 GCAAGC
CelII 48 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
CfoI 53 GCGC GCG^C
1645 GCGC
EcoRII 390 CCTGG ^CC[AT]GG
415 CCAGG
FauI 25 GCGGG CCCGC(4/6)
72 GCGGG
Fnu4HI 8 GCCGC GC^[ACGT]GC
55 GCTGC
HhaI 53 GCGC GCG^C
1645 GCGC
HinP1I 53 GCGC G^CGC
1645 GCGC
HphI 801 GGTGA GGTGA(8/7)
1639 TCACC
ItaI 8 GCCGC GC^[ACGT]GC
55 GCTGC
MvaI 390 CCTGG CC^[AT]GG
415 CCAGG
SfcI 93 CTGCAG C^T[GA][CT]AG
753 CTATAG
TaqI 555 TCGA T^CGA
1439 TCGA
Tsp45I 587 GTCAC ^GT[GC]AC
777 GTCAC
TthHB8I 555 TCGA T^CGA
1439 TCGA
XbaI 766 TCTAGA T^CTAGA
1471 TCTAGA
XmnI 513 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1045 GAAATTGTTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BanII BfaI BmyI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstYI
Cfr13I DdeI DpnII FokI HaeIII
HinfI MaeI MaeIII MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI TfiI
Tru9I Tsp509I TspRI XhoII