Restriction map for
pF1KB8506
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CfoI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NciI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 850 GTAGAC GT^[AC][GT]AC
AhdI 853 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1446 CCTGAGG CC^T[ACGT]AGG
ApaI 2529 GGGCCC GGGCC^C
AseI 1486 ATTAAT AT^TAAT
AsnI 1486 ATTAAT AT^TAAT
Asp700I 2353 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 853 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 743 TGGCCA TGG^CCA
BbsI 342 GAAGAC GAAGAC(2/6)
BbvCI 1347 CCTCAGC CCTCAGC(-5/-2)
BglII 477 AGATCT A^GATCT
BlpI 1567 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1567 GCTGAGC GC^T[ACGT]AGC
BpuAI 342 GAAGAC GAAGAC(2/6)
BsgI 1003 GTGCAG GTGCAG(16/14)
BspLU11I 120 ACATGT A^CATGT
BsrDI 483 CATTGC GCAATG(2/0)
BstXI 2082 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1446 CCTGAGG CC^T[ACGT]AGG
CelII 1567 GCTGAGC GC^T[ACGT]AGC
DraI 115 TTTAAA TTT^AAA
DraIII 1949 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 500 GACTAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 853 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1446 CCTGAGG CC^T[ACGT]AGG
EcoRI 1792 GAATTC G^AATTC
EcoRV 2235 GATATC GAT^ATC
EcoT14I 1301 CCATGG C^C[AT][AT]GG
EcoT22I 2511 ATGCAT ATGCA^T
FauI 2071 GCGGG CCCGC(4/6)
HgaI 939 GCGTC GACGC(5/10)
HindIII 512 AAGCTT A^AGCTT
MluNI 743 TGGCCA TGG^CCA
MscI 743 TGGCCA TGG^CCA
NcoI 1301 CCATGG C^CATGG
NsiI 2511 ATGCAT ATGCA^T
PshAI 934 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1486 ATTAAT AT^TAAT
PstI 1220 CTGCAG CTGCA^G
SacI 1672 GAGCTC GAGCT^C
SapI 766 GAAGAGC GCTCTTC(1/4)
ScaI 911 AGTACT AGT^ACT
SexAI 1950 ACCTGGT A^CC[AT]GGT
StyI 1301 CCATGG C^C[AT][AT]GG
XbaI 75 TCTAGA T^CTAGA
XcmI 2457 CCAGGAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2353 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1147 GAATTT [GA]^AATT[CT]
1792 GAATTC
AflIII 120 ACATGT A^C[GA][CT]GT
130 ACACGT
ApoI 1147 GAATTT [GA]^AATT[CT]
1792 GAATTC
AvaI 1168 CTCGAG C^[CT]CG[GA]G
1425 CTCGAG
BamHI 493 GGATCC G^GATCC
636 GGATCC
BsaI 193 GAGACC GGTCTC(1/5)
860 GGTCTC
BsmAI 193 GAGAC GTCTC(1/5)
861 GTCTC
BsoBI 1168 CTCGAG C^[CT]CG[GA]G
1425 CTCGAG
BsrFI 31 GCCGGC [GA]^CCGG[CT]
1799 GCCGGC
BssSI 1367 CACGAG CACGAG(-5/-1)
1438 CACGAG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
1799 GCCGGC
DsaI 1301 CCATGG C^C[GA][CT]GG
1846 CCACGG
EaeI 743 TGGCCA [CT]^GGCC[GA]
1338 CGGCCA
EcoNI 1611 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2451 CCTAGACCAGG
HapII 32 CCGG C^CGG
1800 CCGG
HpaII 32 CCGG C^CGG
1800 CCGG
MslI 1218 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1702 CACGGATGTG
MspI 32 CCGG C^CGG
1800 CCGG
NaeI 31 GCCGGC GCC^GGC
1799 GCCGGC
NgoMIV 31 GCCGGC G^CCGGC
1799 GCCGGC
NspI 120 ACATGT [GA]CATG^[CT]
421 GCATGT
PaeR7I 1168 CTCGAG C^TCGAG
1425 CTCGAG
PflMI 317 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1948 CCACCTGGTGG
PpuMI 1587 AGGACCT [GA]G^G[AT]CC[CT]
1619 AGGTCCT
PvuII 1249 CAGCTG CAG^CTG
1678 CAGCTG
SmlI 1168 CTCGAG C^T[CT][GA]AG
1425 CTCGAG
SspI 1496 AATATT AAT^ATT
2445 AATATT
Tsp45I 290 GTGAC ^GT[GC]AC
1786 GTGAC
Van91I 317 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1948 CCACCTGGTGG
XhoI 1168 CTCGAG C^TCGAG
1425 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII