Restriction map for
pF1KB5741
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp700I AspEI AvaI AviII AvrII
BamHI BbsI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsiWI BsmBI
BsoBI BspDI BspLU11I BsrDI BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoRV FseI FspI HincII
HindII HindIII HpaI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SmlI SnaBI Sse8387I SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 313 GACGTC GACGT^C
Acc65I 587 GGTACC G^GTACC
AccIII 470 TCCGGA T^CCGGA
AcsI 1038 GAATTT [GA]^AATT[CT]
AlwNI 1528 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1038 GAATTT [GA]^AATT[CT]
AseI 1114 ATTAAT AT^TAAT
AsnI 1114 ATTAAT AT^TAAT
Asp718I 587 GGTACC G^GTACC
AspI 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 15 GGCGCC GGCGC^C
BbrPI 1383 CACGTG CAC^GTG
BbvCI 279 CCTCAGC CCTCAGC(-5/-2)
BclI 1994 TGATCA T^GATCA
BfaI 86 CTAG C^TAG
BsaAI 1383 CACGTG [CT]AC^GT[GA]
BsaBI 1174 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 70 GGTCTC GGTCTC(1/5)
BseAI 470 TCCGGA T^CCGGA
BsmI 1717 GAATGC GAATGC(1/-1)
Bsp1407I 1031 TGTACA T^GTACA
BspEI 470 TCCGGA T^CCGGA
BspHI 2072 TCATGA T^CATGA
BsrBI 1434 GAGCGG CCGCTC(-3/-3)
BsrFI 1335 GCCGGT [GA]^CCGG[CT]
BsrGI 1031 TGTACA T^GTACA
BssHII 47 GCGCGC G^CGCGC
BstEII 1368 GGTCACC G^GT[ACGT]ACC
BstPI 1368 GGTCACC G^GT[ACGT]ACC
Cfr10I 1335 GCCGGT [GA]^CCGG[CT]
EagI 1333 CGGCCG C^GGCCG
EarI 1183 CTCTTC CTCTTC(1/4)
EclXI 1333 CGGCCG C^GGCCG
Eco52I 1333 CGGCCG C^GGCCG
EcoO65I 1368 GGTCACC G^GT[ACGT]ACC
FbaI 1994 TGATCA T^GATCA
KasI 15 GGCGCC G^GCGCC
KpnI 587 GGTACC GGTAC^C
Ksp632I 1183 CTCTTC CTCTTC(1/4)
KspI 4 CCGCGG CCGC^GG
MaeI 86 CTAG C^TAG
MamI 1174 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 470 TCCGGA T^CCGGA
NarI 15 GGCGCC GG^CGCC
NcoI 1695 CCATGG C^CATGG
NdeI 78 CATATG CA^TATG
PflFI 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1383 CACGTG CAC^GTG
PmlI 1383 CACGTG CAC^GTG
PpuMI 1438 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1440 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1114 ATTAAT AT^TAAT
PvuII 1584 CAGCTG CAG^CTG
RcaI 2072 TCATGA T^CATGA
SacI 2092 GAGCTC GAGCT^C
SacII 4 CCGCGG CCGC^GG
ScaI 756 AGTACT AGT^ACT
SexAI 1486 ACCAGGT A^CC[AT]GGT
SfoI 15 GGCGCC GGC^GCC
SpeI 85 ACTAGT A^CTAGT
SphI 1900 GCATGC GCATG^C
SspBI 1031 TGTACA T^GTACA
SspI 1096 AATATT AAT^ATT
StuI 870 AGGCCT AGG^CCT
TfiI 1343 GATTC G^A[AT]TC
Tth111I 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 15 GGCGCC G[GA]^CG[CT]C
313 GACGTC
ApaI 8 GGGCCC GGGCC^C
2288 GGGCCC
BalI 891 TGGCCA TGG^CCA
1794 TGGCCA
BcgI 918 CGAGTACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
482 GCACCAAACTCG
BciVI 1130 GTATCC GTATCC(6/5)
1213 GTATCC
BglII 1625 AGATCT A^GATCT
1799 AGATCT
BpmI 760 CTCCAG CTGGAG(16/14)
1357 CTGGAG
BsaHI 15 GGCGCC G[GA]^CG[CT]C
313 GACGTC
BseRI 267 GAGGAG GAGGAG(10/8)
496 GAGGAG
BsiEI 1333 CGGCCG CG[GA][CT]^CG
2247 CGACCG
BstAPI 1484 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1589 GCAACCAGTGC
BstXI 932 CCAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2165 CCATCCTCCTGG
DraIII 1485 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2341 CACACTGTG
EcoNI 903 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1661 CCTGTGCCAGG
EcoT14I 1695 CCATGG C^C[AT][AT]GG
2060 CCAAGG
EcoT22I 1118 ATGCAT ATGCA^T
1898 ATGCAT
HaeII 15 GGCGCC [GA]GCGC^[CT]
1417 AGCGCC
Hin1I 15 GGCGCC G[GA]^CG[CT]C
313 GACGTC
MaeII 314 ACGT A^CGT
1384 ACGT
MluNI 891 TGGCCA TGG^CCA
1794 TGGCCA
MscI 891 TGGCCA TGG^CCA
1794 TGGCCA
MseI 1115 TTAA T^TAA
1178 TTAA
NsiI 1118 ATGCAT ATGCA^T
1898 ATGCAT
PflMI 883 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2134 CCAGAGTGTGG
PleI 1087 GACTC GAGTC(4/5)
1679 GAGTC
StyI 1695 CCATGG C^C[AT][AT]GG
2060 CCAAGG
Tru9I 1115 TTAA T^TAA
1178 TTAA
Van91I 883 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2134 CCAGAGTGTGG
XcmI 130 CCAAAATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2336 CCACGCACACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII