Restriction map for
pF1KE0026
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 1367 GGCGCC G[GA]^CG[CT]C
AflIII 969 ACGTGT A^C[GA][CT]GT
Alw44I 973 GTGCAC G^TGCAC
ApaLI 973 GTGCAC G^TGCAC
Asp700I 315 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1367 GGCGCC GGCGC^C
BbvCI 1191 GCTGAGG CCTCAGC(-5/-2)
BcnI 1263 CCGGG CC^[GC]GG
BfaI 583 CTAG C^TAG
BglI 210 GCCTAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1367 GGCGCC G[GA]^CG[CT]C
BsaI 664 GAGACC GGTCTC(1/5)
BsaWI 1139 ACCGGA [AT]^CCGG[AT]
BsmBI 943 CGTCTC CGTCTC(1/5)
BspHI 435 TCATGA T^CATGA
BspMI 203 ACCTGC ACCTGC(4/8)
BstEII 283 GGTGACC G^GT[ACGT]ACC
BstPI 283 GGTGACC G^GT[ACGT]ACC
BstXI 8 CCACAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 300 CCATGG C^C[GA][CT]GG
EarI 469 GAAGAG CTCTTC(1/4)
EcoNI 62 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 283 GGTGACC G^GT[ACGT]ACC
Hin1I 1367 GGCGCC G[GA]^CG[CT]C
HindIII 481 AAGCTT A^AGCTT
KasI 1367 GGCGCC G^GCGCC
Ksp632I 469 GAAGAG CTCTTC(1/4)
MaeI 583 CTAG C^TAG
MaeII 969 ACGT A^CGT
MseI 799 TTAA T^TAA
MslI 51 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1067 GCCGGC GCC^GGC
NarI 1367 GGCGCC GG^CGCC
NciI 1263 CCGGG CC^[GC]GG
NcoI 300 CCATGG C^CATGG
NgoMIV 1067 GCCGGC G^CCGGC
PleI 125 GACTC GAGTC(4/5)
PpuMI 716 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 357 CTGCAG CTGCA^G
RcaI 435 TCATGA T^CATGA
SfaNI 1055 GCATC GCATC(5/9)
SfoI 1367 GGCGCC GGC^GCC
Tru9I 799 TTAA T^TAA
Tsp509I 214 AATT ^AATT
XmnI 315 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 1135 GTAGAC GT^[AC][GT]AC
1153 GTCTAC
AocI 66 CCTGAGG CC^T[ACGT]AGG
1061 CCTCAGG
AvaII 717 GGTCC G^G[AT]CC
1251 GGTCC
BamHI 3 GGATCC G^GATCC
921 GGATCC
BanI 1219 GGCACC G^G[CT][GA]CC
1367 GGCGCC
BbsI 855 GAAGAC GAAGAC(2/6)
894 GAAGAC
BpmI 679 CTGGAG CTGGAG(16/14)
847 CTGGAG
BpuAI 855 GAAGAC GAAGAC(2/6)
894 GAAGAC
BsrFI 1067 GCCGGC [GA]^CCGG[CT]
1248 GCCGGT
Bsu36I 66 CCTGAGG CC^T[ACGT]AGG
1061 CCTCAGG
Cfr10I 1067 GCCGGC [GA]^CCGG[CT]
1248 GCCGGT
Eco81I 66 CCTGAGG CC^T[ACGT]AGG
1061 CCTCAGG
FauI 1349 CCCGC CCCGC(4/6)
651 GCGGG
HaeII 296 AGCGCC [GA]GCGC^[CT]
1367 GGCGCC
SacI 182 GAGCTC GAGCT^C
697 GAGCTC
TaqI 180 TCGA T^CGA
446 TCGA
Tsp45I 284 GTGAC ^GT[GC]AC
1315 GTGAC
TthHB8I 180 TCGA T^CGA
446 TCGA
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BalI BanII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfcI StuI StyI
TfiI TseI TspRI XcmI XhoII