Restriction map for
pF1KE0025
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsiEI BsiWI
BspDI BspLU11I BspMI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FseI FspI HpaI KpnI KspI
MaeII MamI MfeI MflI MluI
MslI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 698 TCCGGA T^CCGGA
AcyI 164 GGCGCC G[GA]^CG[CT]C
AspHI 121 GTGCTC G[AT]GC[AT]^C
BalI 68 TGGCCA TGG^CCA
BanI 164 GGCGCC G^G[CT][GA]CC
BbeI 164 GGCGCC GGCGC^C
BcgI 1497 CGAATGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1310 GTATCC GTATCC(6/5)
BcnI 505 CCGGG CC^[GC]GG
BfaI 1549 CTAG C^TAG
BpmI 809 CTGGAG CTGGAG(16/14)
BsaHI 164 GGCGCC G[GA]^CG[CT]C
BsaWI 698 TCCGGA [AT]^CCGG[AT]
BseAI 698 TCCGGA T^CCGGA
BsgI 652 GTGCAG GTGCAG(16/14)
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
BsmBI 1220 GAGACG CGTCTC(1/5)
BsmFI 53 GGGAC GGGAC(10/14)
BsmI 936 GAATGC GAATGC(1/-1)
BspEI 698 TCCGGA T^CCGGA
BsrBI 174 GAGCGG CCGCTC(-3/-3)
BsrDI 18 CATTGC GCAATG(2/0)
EaeI 68 TGGCCA [CT]^GGCC[GA]
EcoNI 1404 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 493 GAATTC G^AATTC
EcoRV 814 GATATC GAT^ATC
FauI 1507 GCGGG CCCGC(4/6)
HaeII 164 GGCGCC [GA]GCGC^[CT]
HgaI 1222 GACGC GACGC(5/10)
Hin1I 164 GGCGCC G[GA]^CG[CT]C
HincII 1036 GTTGAC GT[CT]^[GA]AC
HindII 1036 GTTGAC GT[CT]^[GA]AC
KasI 164 GGCGCC G^GCGCC
MaeI 1549 CTAG C^TAG
MluNI 68 TGGCCA TGG^CCA
MroI 698 TCCGGA T^CCGGA
MscI 68 TGGCCA TGG^CCA
NarI 164 GGCGCC GG^CGCC
NciI 505 CCGGG CC^[GC]GG
PflMI 1243 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 529 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 499 CTGCAG CTGCA^G
ScaI 1029 AGTACT AGT^ACT
SfcI 499 CTGCAG C^T[GA][CT]AG
SfoI 164 GGCGCC GGC^GCC
StuI 213 AGGCCT AGG^CCT
Tsp45I 445 GTGAC ^GT[GC]AC
Van91I 1243 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 567 CCAGGTTGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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BanII 718 GAGCCC G[GA]GC[CT]^C
1634 GAGCCC
BclI 594 TGATCA T^GATCA
1107 TGATCA
BsaI 154 GGTCTC GGTCTC(1/5)
885 GGTCTC
BseRI 157 CTCCTC GAGGAG(10/8)
1046 GAGGAG
Bsp1407I 343 TGTACA T^GTACA
1661 TGTACA
BspHI 591 TCATGA T^CATGA
689 TCATGA
BsrGI 343 TGTACA T^GTACA
1661 TGTACA
BstAPI 955 GCAAATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1579 GCAATCTATGC
CfoI 165 GCGC GCG^C
291 GCGC
DpnII 595 GATC ^GATC
1108 GATC
DraI 949 TTTAAA TTT^AAA
1273 TTTAAA
DraII 80 AGGTCCT [GA]G^G[ACGT]CC[CT]
1509 GGGTCCT
EcoO109I 80 AGGTCCT [GA]G^G[ACGT]CC[CT]
1509 GGGTCCT
FbaI 594 TGATCA T^GATCA
1107 TGATCA
HapII 505 CCGG C^CGG
699 CCGG
HhaI 165 GCGC GCG^C
291 GCGC
HinP1I 165 GCGC G^CGC
291 GCGC
HindIII 321 AAGCTT A^AGCTT
1170 AAGCTT
HpaII 505 CCGG C^CGG
699 CCGG
MboI 595 GATC ^GATC
1108 GATC
MspI 505 CCGG C^CGG
699 CCGG
NdeII 595 GATC ^GATC
1108 GATC
PleI 693 GAGTC GAGTC(4/5)
987 GAGTC
PpuMI 80 AGGTCCT [GA]G^G[AT]CC[CT]
1509 GGGTCCT
PvuII 335 CAGCTG CAG^CTG
416 CAGCTG
RcaI 591 TCATGA T^CATGA
689 TCATGA
SapI 662 GAAGAGC GCTCTTC(1/4)
123 GCTCTTC
Sau3AI 595 GATC ^GATC
1108 GATC
SspBI 343 TGTACA T^GTACA
1661 TGTACA
SspI 307 AATATT AAT^ATT
1680 AATATT
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
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AciI AcsI AfaI AluI ApoI
AvaI AvaII BbvI BmyI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
NlaIV PaeR7I RsaI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoI