Restriction map for
pF1KE0014
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BbeI BcgI BcnI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MflI MluI MroI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 96 GACGTC GACGT^C
AccII 108 CGCG CG^CG
AflII 1342 CTTAAG C^TTAAG
ApaI 598 GGGCCC GGGCC^C
BalI 1371 TGGCCA TGG^CCA
BbrPI 1326 CACGTG CAC^GTG
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
BclI 131 TGATCA T^GATCA
BfaI 721 CTAG C^TAG
BfrI 1342 CTTAAG C^TTAAG
BglI 1027 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1246 CTCCAG CTGGAG(16/14)
BseRI 766 CTCCTC GAGGAG(10/8)
BsrFI 400 ACCGGC [GA]^CCGG[CT]
BstAPI 1119 GCACGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 151 GGTTACC G^GT[ACGT]ACC
BstPI 151 GGTTACC G^GT[ACGT]ACC
BstUI 108 CGCG CG^CG
BstXI 140 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 400 ACCGGC [GA]^CCGG[CT]
DraIII 431 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 151 GGTTACC G^GT[ACGT]ACC
EcoRV 412 GATATC GAT^ATC
EcoT22I 882 ATGCAT ATGCA^T
FbaI 131 TGATCA T^GATCA
HapII 401 CCGG C^CGG
HincII 469 GTTGAC GT[CT]^[GA]AC
HindII 469 GTTGAC GT[CT]^[GA]AC
HpaII 401 CCGG C^CGG
HphI 854 TCACC GGTGA(8/7)
MaeI 721 CTAG C^TAG
MfeI 239 CAATTG C^AATTG
MluNI 1371 TGGCCA TGG^CCA
MscI 1371 TGGCCA TGG^CCA
MspI 401 CCGG C^CGG
MunI 239 CAATTG C^AATTG
MvnI 108 CGCG CG^CG
NsiI 882 ATGCAT ATGCA^T
PmaCI 1326 CACGTG CAC^GTG
PmlI 1326 CACGTG CAC^GTG
PstI 55 CTGCAG CTGCA^G
SapI 1287 GCTCTTC GCTCTTC(1/4)
SexAI 1139 ACCTGGT A^CC[AT]GGT
SfcI 55 CTGCAG C^T[GA][CT]AG
StuI 231 AGGCCT AGG^CCT
TaqI 312 TCGA T^CGA
TfiI 615 GAATC G^A[AT]TC
TthHB8I 312 TCGA T^CGA
XcmI 1022 CCAACGCCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AcsI 498 GAATTC [GA]^AATT[CT]
1159 GAATTC
AcyI 3 GGCGTC G[GA]^CG[CT]C
96 GACGTC
AfaI 813 GTAC GT^AC
843 GTAC
AlwNI 70 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
118 CAGGCGCTG
ApoI 498 GAATTC [GA]^AATT[CT]
1159 GAATTC
BanI 994 GGCACC G^G[CT][GA]CC
1428 GGTGCC
BanII 598 GGGCCC G[GA]GC[CT]^C
1209 GAGCCC
BbsI 757 GAAGAC GAAGAC(2/6)
775 GAAGAC
BciVI 382 GGATAC GTATCC(6/5)
1135 GGATAC
BlpI 75 GCTGAGC GC^T[ACGT]AGC
738 GCTCAGC
BmyI 598 GGGCCC G[AGT]GC[ACT]^C
1209 GAGCCC
Bpu1102I 75 GCTGAGC GC^T[ACGT]AGC
738 GCTCAGC
BpuAI 757 GAAGAC GAAGAC(2/6)
775 GAAGAC
BsaAI 533 TACGTG [CT]AC^GT[GA]
1326 CACGTG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
96 GACGTC
BsaI 196 GAGACC GGTCTC(1/5)
1090 GGTCTC
BsgI 1126 CTGCAC GTGCAG(16/14)
634 GTGCAG
BsmAI 196 GAGAC GTCTC(1/5)
1091 GTCTC
Bsp1286I 598 GGGCCC G[AGT]GC[ACT]^C
1209 GAGCCC
Bsp1407I 812 TGTACA T^GTACA
842 TGTACA
BsrDI 999 CATTGC GCAATG(2/0)
1423 GCAATG
BsrGI 812 TGTACA T^GTACA
842 TGTACA
CelII 75 GCTGAGC GC^T[ACGT]AGC
738 GCTCAGC
EaeI 1229 TGGCCG [CT]^GGCC[GA]
1371 TGGCCA
EcoRI 498 GAATTC G^AATTC
1159 GAATTC
HgaI 4 GCGTC GACGC(5/10)
109 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
96 GACGTC
MslI 810 CATGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
954 CATGAAGGTG
MspA1I 11 CAGCGG C[AC]G^C[GT]G
804 CCGCTG
NspI 809 GCATGT [GA]CATG^[CT]
815 ACATGC
PflMI 1022 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCATCCACTGG
PleI 1055 GACTC GAGTC(4/5)
702 GAGTC
RsaI 813 GTAC GT^AC
843 GTAC
SfaNI 940 GATGC GCATC(5/9)
677 GCATC
SspBI 812 TGTACA T^GTACA
842 TGTACA
Tsp45I 853 GTCAC ^GT[GC]AC
868 GTCAC
Van91I 1022 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCATCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SmlI StyI Tru9I TseI Tsp509I
TspRI