Restriction map for
pF1KA0911
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII KasI KpnI MfeI
MluI MroI MunI NarI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI ScaI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1674 GTATAC GT^[AC][GT]AC
AflIII 871 ACATGT A^C[GA][CT]GT
BciVI 2151 GGATAC GTATCC(6/5)
BfaI 913 CTAG C^TAG
BglI 1967 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1930 TACGTG [CT]AC^GT[GA]
BsaBI 1234 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2746 GAGACC GGTCTC(1/5)
BsaWI 2360 ACCGGA [AT]^CCGG[AT]
BspHI 2618 TCATGA T^CATGA
BspLU11I 871 ACATGT A^CATGT
Bst1107I 1674 GTATAC GTA^TAC
BstAPI 2316 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1674 GTATAC GTA^TAC
DraI 259 TTTAAA TTT^AAA
HaeII 2839 AGCGCC [GA]GCGC^[CT]
HpaI 91 GTTAAC GTT^AAC
KspI 992 CCGCGG CCGC^GG
MaeI 913 CTAG C^TAG
MamI 1234 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1274 GCCGGC GCC^GGC
NgoMIV 1274 GCCGGC G^CCGGC
NotI 988 GCGGCCGC GC^GGCCGC
PflMI 2532 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2911 CAGCTG CAG^CTG
RcaI 2618 TCATGA T^CATGA
SacII 992 CCGCGG CCGC^GG
SbfI 1698 CCTGCAGG CCTGCA^GG
SexAI 1079 ACCAGGT A^CC[AT]GGT
SgrAI 831 CACCGGCG C[GA]^CCGG[CT]G
SphI 2340 GCATGC GCATG^C
Sse8387I 1698 CCTGCAGG CCTGCA^GG
StuI 386 AGGCCT AGG^CCT
Van91I 2532 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 1126 GGCGTC G[GA]^CG[CT]C
1975 GGCGTC
Alw44I 2140 GTGCAC G^TGCAC
2494 GTGCAC
AlwNI 1997 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2908 CAGCAGCTG
ApaLI 2140 GTGCAC G^TGCAC
2494 GTGCAC
BbsI 1711 GAAGAC GAAGAC(2/6)
1777 GAAGAC
BbvCI 2934 CCTCAGC CCTCAGC(-5/-2)
2095 GCTGAGG
BpuAI 1711 GAAGAC GAAGAC(2/6)
1777 GAAGAC
BsaHI 1126 GGCGTC G[GA]^CG[CT]C
1975 GGCGTC
DrdI 877 GACGAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1696 GACCTGCAGGTC
EagI 32 CGGCCG C^GGCCG
989 CGGCCG
EclXI 32 CGGCCG C^GGCCG
989 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
989 CGGCCG
EcoRI 1396 GAATTC G^AATTC
1850 GAATTC
Hin1I 1126 GGCGTC G[GA]^CG[CT]C
1975 GGCGTC
MspA1I 992 CCGCGG C[AC]G^C[GT]G
2911 CAGCTG
NspI 871 ACATGT [GA]CATG^[CT]
2340 GCATGC
XcmI 335 CCAAAGAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
972 CCACCGGCTCTGTGG
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII