Restriction map for
pF1KE0006
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwNI 934 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 214 AGCGCT AGC^GCT
AseI 463 ATTAAT AT^TAAT
AsnI 463 ATTAAT AT^TAAT
Asp700I 886 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 654 CTCGGG C^[CT]CG[GA]G
AvaII 190 GGACC G^G[AT]CC
BalI 323 TGGCCA TGG^CCA
BamHI 833 GGATCC G^GATCC
BcgI 1059 CGACATGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 448 CTAG C^TAG
BpmI 40 CTGGAG CTGGAG(16/14)
BsaBI 1270 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 654 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1416 TGTACA T^GTACA
BsrGI 1416 TGTACA T^GTACA
CfoI 215 GCGC GCG^C
Cfr13I 190 GGACC G^G[ACGT]CC
DraII 189 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1207 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 323 TGGCCA [CT]^GGCC[GA]
Eco47III 214 AGCGCT AGC^GCT
EcoO109I 189 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 520 GATATC GAT^ATC
FauI 174 GCGGG CCCGC(4/6)
HaeII 214 AGCGCT [GA]GCGC^[CT]
HapII 831 CCGG C^CGG
HgaI 24 GCGTC GACGC(5/10)
HhaI 215 GCGC GCG^C
HinP1I 215 GCGC G^CGC
HincII 627 GTTGAC GT[CT]^[GA]AC
HindII 627 GTTGAC GT[CT]^[GA]AC
HpaII 831 CCGG C^CGG
MaeI 448 CTAG C^TAG
MamI 1270 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
MspI 831 CCGG C^CGG
PleI 73 GACTC GAGTC(4/5)
PpuMI 189 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 463 ATTAAT AT^TAAT
Sau96I 190 GGACC G^G[ACGT]CC
ScaI 969 AGTACT AGT^ACT
SphI 474 GCATGC GCATG^C
SspBI 1416 TGTACA T^GTACA
SspI 1496 AATATT AAT^ATT
TaqI 1058 TCGA T^CGA
TthHB8I 1058 TCGA T^CGA
XmnI 886 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccI 70 GTAGAC GT^[AC][GT]AC
1450 GTAGAC
AluI 66 AGCT AG^CT
1010 AGCT
BsaI 590 GAGACC GGTCTC(1/5)
672 GAGACC
BsiYI 749 CCAACCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1308 CCAGGACATGG
BslI 749 CCAACCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1308 CCAGGACATGG
BsrDI 1185 CATTGC GCAATG(2/0)
647 GCAATG
DsaI 185 CCGTGG C^C[GA][CT]GG
390 CCACGG
EarI 816 GAAGAG CTCTTC(1/4)
1033 GAAGAG
HaeIII 324 GGCC GG^CC
1053 GGCC
Ksp632I 816 GAAGAG CTCTTC(1/4)
1033 GAAGAG
MspA1I 196 CAGCGG C[AC]G^C[GT]G
622 CAGCGG
PflMI 749 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1308 CCAGGACATGG
SmlI 712 CTCAAG C^T[CT][GA]AG
1006 CTCAAG
Van91I 749 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1308 CCAGGACATGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AciI AcsI AfaI AlwI ApoI
BbvI BsaJI BseRI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII