##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BcgI        
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BmyI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TaqI        Tru9I       Tsp509I     
TspRI       Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

Acc65I        444  GGTACC               G^GTACC
AocI           85  CCTCAGG              CC^T[ACGT]AGG
Asp718I       444  GGTACC               G^GTACC
BbsI          492  GAAGAC               GAAGAC(2/6)
BbvCI          40  CCTCAGC              CCTCAGC(-5/-2)
BciVI         409  GGATAC               GTATCC(6/5)
BlpI          484  GCTCAGC              GC^T[ACGT]AGC
BpmI          473  CTCCAG               CTGGAG(16/14)
Bpu1102I      484  GCTCAGC              GC^T[ACGT]AGC
BpuAI         492  GAAGAC               GAAGAC(2/6)
BspMI          57  GCAGGT               ACCTGC(4/8)
Bsu36I         85  CCTCAGG              CC^T[ACGT]AGG
Cac8I          74  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         484  GCTCAGC              GC^T[ACGT]AGC
Eco81I         85  CCTCAGG              CC^T[ACGT]AGG
HaeIII        478  GGCC                 GG^CC
HinfI         156  GATTC                G^A[ACGT]TC
HphI           60  GGTGA                GGTGA(8/7)
KpnI          444  GGTACC               GGTAC^C
MaeIII        469  GTCAC                ^GT[ACGT]AC
MboII         492  GAAGA                GAAGA(8/7)
MwoI           45  GCTTCAACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         136  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI         341  GCATC                GCATC(5/9)
SspI           93  AATATT               AAT^ATT
TfiI          156  GATTC                G^A[AT]TC
Tsp45I        469  GTCAC                ^GT[GC]AC
Van91I        136  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           44  AGCT                 AG^CT
              483  AGCT                
BanI          385  GGTGCC               G^G[CT][GA]CC
              444  GGTACC              
BbvI           54  GCAGC                GCAGC(8/12)
              338  GCAGC               
Fnu4HI         54  GCAGC                GC^[ACGT]GC
              338  GCAGC               
ItaI           54  GCAGC                GC^[ACGT]GC
              338  GCAGC               
NlaIII        119  CATG                 CATG^
              455  CATG                
TseI           54  GCAGC                G^C[AT]GC
              338  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        AvaII       BsaJI       BsiYI       BslI        
BsmFI       BssKI       BstNI       Cfr13I      DdeI        
DraII       EcoNI       EcoO109I    EcoRII      EcoT14I     
MnlI        MvaI        NlaIV       PpuMI       RsaI        
Sau96I      ScrFI       StyI