Restriction map for
pF1KB9975
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI DraI DraIII
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MfeI MluI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacI SalI SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tth111I XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 205 GACGTC GACGT^C
AccI 1078 GTCTAC GT^[AC][GT]AC
AccIII 188 TCCGGA T^CCGGA
AcsI 1005 AAATTC [GA]^AATT[CT]
ApaI 437 GGGCCC GGGCC^C
ApoI 1005 AAATTC [GA]^AATT[CT]
AspHI 547 GTGCTC G[AT]GC[AT]^C
AviII 758 TGCGCA TGC^GCA
BanI 916 GGCGCC G^G[CT][GA]CC
BbeI 916 GGCGCC GGCGC^C
BbsI 408 GAAGAC GAAGAC(2/6)
BpuAI 408 GAAGAC GAAGAC(2/6)
BsaI 1569 GGTCTC GGTCTC(1/5)
BsaWI 188 TCCGGA [AT]^CCGG[AT]
BseAI 188 TCCGGA T^CCGGA
BsiEI 506 CGGCCG CG[GA][CT]^CG
BsiHKAI 547 GTGCTC G[AT]GC[AT]^C
BspEI 188 TCCGGA T^CCGGA
BspHI 89 TCATGA T^CATGA
BsrDI 1427 GCAATG GCAATG(2/0)
BstAPI 1188 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 320 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 78 CGGACCG CG^G[AT]CCG
DrdI 4 GACGGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 506 CGGCCG C^GGCCG
EarI 571 CTCTTC CTCTTC(1/4)
EclXI 506 CGGCCG C^GGCCG
Eco52I 506 CGGCCG C^GGCCG
EcoNI 1275 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 758 TGCGCA TGC^GCA
KasI 916 GGCGCC G^GCGCC
Ksp632I 571 CTCTTC CTCTTC(1/4)
KspI 881 CCGCGG CCGC^GG
MroI 188 TCCGGA T^CCGGA
NarI 916 GGCGCC GG^CGCC
PflMI 870 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1043 GAGTC GAGTC(4/5)
PpuMI 1412 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 89 TCATGA T^CATGA
RsrII 78 CGGACCG CG^G[AT]CCG
SacII 881 CCGCGG CCGC^GG
SbfI 450 CCTGCAGG CCTGCA^GG
SfoI 916 GGCGCC GGC^GCC
SmaI 984 CCCGGG CCC^GGG
SmlI 1408 CTCAAG C^T[CT][GA]AG
Sse8387I 450 CCTGCAGG CCTGCA^GG
StuI 287 AGGCCT AGG^CCT
TfiI 1448 GATTC G^A[AT]TC
Tsp509I 1006 AATT ^AATT
Van91I 870 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 172 TCTAGA T^CTAGA
XmaCI 984 CCCGGG C^CCGGG
XmaI 984 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 32 GTAC GT^AC
798 GTAC
AlwNI 667 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1270 CAGGGCCTG
Asp700I 567 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1341 GAACATATTC
AvaI 984 CCCGGG C^[CT]CG[GA]G
1546 CTCGGG
BalI 932 TGGCCA TGG^CCA
1166 TGGCCA
BfaI 173 CTAG C^TAG
1132 CTAG
BsaBI 180 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
975 GATCGCCATC
BsmAI 1393 GTCTC GTCTC(1/5)
1570 GTCTC
BsoBI 984 CCCGGG C^[CT]CG[GA]G
1546 CTCGGG
BstEII 252 GGTCACC G^GT[ACGT]ACC
348 GGTGACC
BstPI 252 GGTCACC G^GT[ACGT]ACC
348 GGTGACC
DdeI 550 CTCAG C^T[ACGT]AG
1389 CTCAG
EcoO65I 252 GGTCACC G^GT[ACGT]ACC
348 GGTGACC
HaeII 697 AGCGCC [GA]GCGC^[CT]
916 GGCGCC
HinfI 1043 GAGTC G^A[ACGT]TC
1448 GATTC
MaeI 173 CTAG C^TAG
1132 CTAG
MamI 180 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
975 GATCGCCATC
MluNI 932 TGGCCA TGG^CCA
1166 TGGCCA
MscI 932 TGGCCA TGG^CCA
1166 TGGCCA
NcoI 326 CCATGG C^CATGG
875 CCATGG
RsaI 32 GTAC GT^AC
798 GTAC
TspRI 626 CAGTG CA[GC]TG[ACGT][ACGT]^
1481 CACTG
XcmI 710 CCAAGCTGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
980 CCATCCCGGGCCTGG
XmnI 567 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1341 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI StyI TaqI TseI
Tsp45I TthHB8I XhoII