Restriction map for
pF1KB9970
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI DraI
DraIII Eam1105I Eco81I EcoO65I EcoRI
EcoT14I FbaI FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp509I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1369 GTCTAC GT^[AC][GT]AC
AcyI 1223 GGCGCC G[GA]^CG[CT]C
AflIII 995 ACACGT A^C[GA][CT]GT
Alw44I 1777 GTGCAC G^TGCAC
Aor51HI 1211 AGCGCT AGC^GCT
ApaLI 1777 GTGCAC G^TGCAC
AspI 1717 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1219 CCCGGG C^[CT]CG[GA]G
BamHI 850 GGATCC G^GATCC
BanI 1223 GGCGCC G^G[CT][GA]CC
BbeI 1223 GGCGCC GGCGC^C
BbrPI 996 CACGTG CAC^GTG
BfaI 907 CTAG C^TAG
BsaAI 996 CACGTG [CT]AC^GT[GA]
BsaHI 1223 GGCGCC G[GA]^CG[CT]C
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 2091 CGTCTC CGTCTC(1/5)
BsoBI 1219 CCCGGG C^[CT]CG[GA]G
BsrBI 1683 CCGCTC CCGCTC(-3/-3)
BsrDI 331 CATTGC GCAATG(2/0)
BstYI 850 GGATCC [GA]^GATC[CT]
CpoI 1721 CGGTCCG CG^G[AT]CCG
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco47III 1211 AGCGCT AGC^GCT
Eco52I 71 CGGCCG C^GGCCG
EcoNI 93 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 922 GATATC GAT^ATC
HgaI 82 GACGC GACGC(5/10)
Hin1I 1223 GGCGCC G[GA]^CG[CT]C
HindIII 1509 AAGCTT A^AGCTT
KasI 1223 GGCGCC G^GCGCC
MaeI 907 CTAG C^TAG
MflI 850 GGATCC [GA]^GATC[CT]
NarI 1223 GGCGCC GG^CGCC
NdeI 158 CATATG CA^TATG
PflFI 1717 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 996 CACGTG CAC^GTG
PmlI 996 CACGTG CAC^GTG
PstI 1269 CTGCAG CTGCA^G
RsrII 1721 CGGTCCG CG^G[AT]CCG
SacI 757 GAGCTC GAGCT^C
ScaI 2099 AGTACT AGT^ACT
SfoI 1223 GGCGCC GGC^GCC
SmaI 1219 CCCGGG CCC^GGG
SphI 424 GCATGC GCATG^C
StuI 101 AGGCCT AGG^CCT
Tth111I 1717 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 850 GGATCC [GA]^GATC[CT]
XmaCI 1219 CCCGGG C^CCGGG
XmaI 1219 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 381 GTAC GT^AC
2100 GTAC
AlwI 851 GATCC GGATC(4/5)
850 GGATC
ApaI 550 GGGCCC GGGCC^C
2001 GGGCCC
AspHI 757 GAGCTC G[AT]GC[AT]^C
1777 GTGCAC
BlpI 1090 GCTGAGC GC^T[ACGT]AGC
1410 GCTCAGC
Bpu1102I 1090 GCTGAGC GC^T[ACGT]AGC
1410 GCTCAGC
BsaWI 122 ACCGGA [AT]^CCGG[AT]
448 ACCGGA
BsiHKAI 757 GAGCTC G[AT]GC[AT]^C
1777 GTGCAC
BsrFI 680 GCCGGC [GA]^CCGG[CT]
1394 GCCGGC
BssHII 6 GCGCGC G^CGCGC
1079 GCGCGC
CelII 1090 GCTGAGC GC^T[ACGT]AGC
1410 GCTCAGC
Cfr10I 680 GCCGGC [GA]^CCGG[CT]
1394 GCCGGC
DrdI 82 GACGCGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
583 GACGAGGTGGTC
EarI 318 GAAGAG CTCTTC(1/4)
1837 GAAGAG
EcoT22I 657 ATGCAT ATGCA^T
972 ATGCAT
HaeII 1211 AGCGCT [GA]GCGC^[CT]
1223 GGCGCC
HincII 1198 GTCAAC GT[CT]^[GA]AC
2074 GTCAAC
HindII 1198 GTCAAC GT[CT]^[GA]AC
2074 GTCAAC
Ksp632I 318 GAAGAG CTCTTC(1/4)
1837 GAAGAG
NaeI 680 GCCGGC GCC^GGC
1394 GCCGGC
NgoMIV 680 GCCGGC G^CCGGC
1394 GCCGGC
NsiI 657 ATGCAT ATGCA^T
972 ATGCAT
PleI 1927 GACTC GAGTC(4/5)
1427 GAGTC
PpuMI 26 GGGTCCC [GA]G^G[AT]CC[CT]
515 AGGACCC
RsaI 381 GTAC GT^AC
2100 GTAC
TfiI 169 GAATC G^A[AT]TC
691 GAATC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BalI BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I