Restriction map for
pF1KB9965
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AlwNI ApaI AscI
AseI AsnI AspEI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstXI BstZ17I
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI KasI
MamI MluI MluNI MroI MscI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 199 GGTACC G^GTACC
AccI 297 GTCTAC GT^[AC][GT]AC
AccII 29 CGCG CG^CG
AcyI 166 GACGCC G[GA]^CG[CT]C
Alw44I 1139 GTGCAC G^TGCAC
AocI 672 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1133 AGCGCT AGC^GCT
ApaLI 1139 GTGCAC G^TGCAC
Asp718I 199 GGTACC G^GTACC
AspHI 1139 GTGCAC G[AT]GC[AT]^C
AspI 1359 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1215 GCAGATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 89 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1021 CTCCAG CTGGAG(16/14)
BsaHI 166 GACGCC G[GA]^CG[CT]C
BsgI 49 CTGCAC GTGCAG(16/14)
BsiHKAI 1139 GTGCAC G[AT]GC[AT]^C
BspDI 502 ATCGAT AT^CGAT
BsrFI 53 ACCGGC [GA]^CCGG[CT]
BstAPI 80 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 29 CGCG CG^CG
Bsu36I 672 CCTCAGG CC^T[ACGT]AGG
Cfr10I 53 ACCGGC [GA]^CCGG[CT]
ClaI 502 ATCGAT AT^CGAT
DraII 1085 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 81 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 28 CCGCGG C^C[GA][CT]GG
Eco47III 1133 AGCGCT AGC^GCT
Eco81I 672 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1085 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 788 GAATTC G^AATTC
EcoT14I 1034 CCTTGG C^C[AT][AT]GG
HgaI 166 GACGC GACGC(5/10)
Hin1I 166 GACGCC G[GA]^CG[CT]C
HincII 1546 GTTAAC GT[CT]^[GA]AC
HindII 1546 GTTAAC GT[CT]^[GA]AC
HindIII 848 AAGCTT A^AGCTT
HpaI 1546 GTTAAC GTT^AAC
KpnI 199 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MfeI 479 CAATTG C^AATTG
MspA1I 28 CCGCGG C[AC]G^C[GT]G
MunI 479 CAATTG C^AATTG
MvnI 29 CGCG CG^CG
NdeI 1532 CATATG CA^TATG
NspI 1281 GCATGC [GA]CATG^[CT]
PflFI 1359 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1108 CTGCAG CTGCA^G
SacII 28 CCGCGG CCGC^GG
SapI 514 GCTCTTC GCTCTTC(1/4)
ScaI 558 AGTACT AGT^ACT
SphI 1281 GCATGC GCATG^C
SspI 984 AATATT AAT^ATT
StuI 899 AGGCCT AGG^CCT
StyI 1034 CCTTGG C^C[AT][AT]GG
Tth111I 1359 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 573 CCACAAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Asp700I 511 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1007 GAACTGATTC
BanI 79 GGCACC G^G[CT][GA]CC
199 GGTACC
BbsI 1356 GAAGAC GAAGAC(2/6)
1469 GTCTTC
BcnI 66 CCCGG CC^[GC]GG
119 CCCGG
BfaI 734 CTAG C^TAG
949 CTAG
BlpI 432 GCTAAGC GC^T[ACGT]AGC
724 GCTCAGC
Bpu1102I 432 GCTAAGC GC^T[ACGT]AGC
724 GCTCAGC
BpuAI 1356 GAAGAC GAAGAC(2/6)
1469 GTCTTC
BsmFI 464 GTCCC GGGAC(10/14)
1643 GTCCC
BsmI 262 GAATGC GAATGC(1/-1)
372 GCATTC
BstBI 227 TTCGAA TT^CGAA
1595 TTCGAA
BstEII 1026 GGTGACC G^GT[ACGT]ACC
1117 GGTCACC
BstPI 1026 GGTGACC G^GT[ACGT]ACC
1117 GGTCACC
BstYI 1217 AGATCC [GA]^GATC[CT]
1507 AGATCC
CelII 432 GCTAAGC GC^T[ACGT]AGC
724 GCTCAGC
CfoI 549 GCGC GCG^C
1134 GCGC
EarI 515 CTCTTC CTCTTC(1/4)
1195 CTCTTC
EcoO65I 1026 GGTGACC G^GT[ACGT]ACC
1117 GGTCACC
FauI 27 CCCGC CCCGC(4/6)
30 GCGGG
HaeII 548 GGCGCT [GA]GCGC^[CT]
1133 AGCGCT
HhaI 549 GCGC GCG^C
1134 GCGC
HinP1I 549 GCGC G^CGC
1134 GCGC
Ksp632I 515 CTCTTC CTCTTC(1/4)
1195 CTCTTC
MaeI 734 CTAG C^TAG
949 CTAG
MaeII 719 ACGT A^CGT
1497 ACGT
MflI 1217 AGATCC [GA]^GATC[CT]
1507 AGATCC
MslI 315 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1282 CATGCTTATG
NciI 66 CCCGG CC^[GC]GG
119 CCCGG
NspV 227 TTCGAA TT^CGAA
1595 TTCGAA
PflMI 419 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
613 CCACTCACTGG
SfcI 1108 CTGCAG C^T[GA][CT]AG
1348 CTACAG
SfuI 227 TTCGAA TT^CGAA
1595 TTCGAA
Van91I 419 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
613 CCACTCACTGG
XhoII 1217 AGATCC [GA]^GATC[CT]
1507 AGATCC
XmnI 511 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1007 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I